Signal Amplification of Nonextractive Immunoassay

EPA Contract Number: 68D60030
Title: Signal Amplification of Nonextractive Immunoassay
Investigators: Thomas, Rhys N.
Small Business: Fayette Environmental Services Inc.
EPA Contact: Manager, SBIR Program
Phase: I
Project Period: September 1, 1996 through March 1, 1997
Project Amount: $70,000
RFA: Small Business Innovation Research (SBIR) - Phase I (1996) RFA Text |  Recipients Lists
Research Category: Ecological Indicators/Assessment/Restoration , SBIR - Monitoring , Small Business Innovation Research (SBIR)


Nonextractive immunoassay for analysis of soils and sediments for compounds of environmental interest has been shown to be sensitive and precise for most applications. Of particular value is the fact that nonextractive immunoassay uses no toxic solvents and generates no wastes. Being a noncompetitive enzyme-linked immunoassay, the detection limit is governed by the ability to differentiate small quantities of signal from the background. An increase in signal strength of several orders of magnitude may be gained by covalently binding novel, signal-amplifying functionalities to the antibodies. This project will develop and evaluate a signal amplified nonextractive immunoassay for inexpensive field assay of contaminated soils and sediments with detection limits in the low part per trillion range.

Supplemental Keywords:

small business, SBIR, engineering, chemistry, biosensors, pesticide residues, hazardous waste., RFA, Scientific Discipline, Toxics, Waste, Water, Ecosystem Protection/Environmental Exposure & Risk, Contaminated Sediments, Environmental Chemistry, Chemistry, pesticides, Monitoring/Modeling, Analytical Chemistry, Environmental Monitoring, Environmental Engineering, biosensing, monitoring, nonextractive immunoassay, sediment bioassay, sediment treatment, assays, field portable monitoring, contaminated sediment, hazardous waste, biosensing system, remediation, pesticide residue, signal amplification, biosensor, biosensors

Progress and Final Reports:

  • Final
  • SBIR Phase II:

    Signal Amplification of Nonextractive Immunoassay