A Simplified Biosensor for Pesticide Residue Analysis

EPA Contract Number: 68D60024
Title: A Simplified Biosensor for Pesticide Residue Analysis
Investigators: Sand, Theodore T.
Small Business: DISAN Inc. - HTI Bio-Products Inc.
EPA Contact: Manager, SBIR Program
Phase: I
Project Period: September 1, 1996 through March 1, 1997
Project Amount: $70,000
RFA: Small Business Innovation Research (SBIR) - Phase I (1996) RFA Text |  Recipients Lists
Research Category: Ecological Indicators/Assessment/Restoration , SBIR - Monitoring , Small Business Innovation Research (SBIR)


The monitoring of pesticide residues currently is accomplished in fixed laboratory facilities. The methods, while comprehensive, usually involve extensive sample preparation and concentration prior to analysis. There is a growing need to move pesticide residue analysis into the field. Biosensors based on immunoassay procedures offer an excellent format to accomplish this goal. Simplified assays with as few steps and liquid reagents as possible would help support a move to in-field analysis. The proposed effort will lead to the development of this type of biosensor. The objectives to be achieved include selection of an enzyme and method of conjugation and evaluation of various configurations of a means to electronically transduce the signal. Availability of the proposed, simplified biosensor should meet the need for rugged, easy-to-use, low-cost diagnostic tools for in-field analysis. The proposed technology should support the trend of "faster, cheaper, safer, better" with regard toscreening for priority pollutants in a variety of matrices.

Supplemental Keywords:

small business, SBIR, engineering, chemistry, hazardous waste., RFA, Scientific Discipline, Toxics, Waste, Ecosystem Protection/Environmental Exposure & Risk, Environmental Chemistry, Chemistry, pesticides, Monitoring/Modeling, Analytical Chemistry, Hazardous Waste, Hazardous, Environmental Engineering, environmental monitoring, monitoring, agricultural runoff, immunoassay, field portable monitoring, hazardous chemicals, pesticide residue

Progress and Final Reports:

  • Final