Cryptosporidium Detection System

EPA Contract Number: 68D01012
Title: Cryptosporidium Detection System
Investigators: Fernandez, Salvador M.
Small Business: Ciencia Inc.
EPA Contact: Manager, SBIR Program
Phase: I
Project Period: April 1, 2001 through September 1, 2001
Project Amount: $70,000
RFA: Small Business Innovation Research (SBIR) - Phase I (2001) RFA Text |  Recipients Lists
Research Category: Ecological Indicators/Assessment/Restoration , SBIR - Monitoring , Small Business Innovation Research (SBIR)


The Phase I objective is to develop a compact, low-cost, easy-to-use system for the rapid detection of Cryptosporidium oocysts. Two key innovations provide the foundation for Ciencia, Inc.'s system: (1) a dielectrophoretic field-flow-fractionation method to replace current filtering and centrifugation methods to concentrate the sample and remove nontarget organisms or nonbiological debris, and (2) direct fluorescent immunoassay detection using a novel type of fluorescent label that should result in a sensitivity improvement of the order of 104 over that of conventional fluorescent immunoassays. Fluorescence excitation will be provided by single quantum well light-emitting diodes. This will result in a low-cost, compact instrument of very low power consumption. These innovations can be used to improve current laboratory-based procedures for Cryptosporidium detection, but more importantly, they can be exploited to implement portable devices for rapid onsite detection, and offer the potential of implementation of online sensors.

Supplemental Keywords:

small business, SBIR, monitoring, Cryptosporidium, engineering, EPA, sensors, drinking water., RFA, Scientific Discipline, Water, Ecosystem Protection/Environmental Exposure & Risk, exploratory research environmental biology, Ecosystem/Assessment/Indicators, Chemistry, Monitoring/Modeling, Ecological Effects - Environmental Exposure & Risk, Ecological Effects - Human Health, Environmental Monitoring, Drinking Water, Engineering, Environmental Engineering, Ecological Indicators, cryptosporidium parvum oocysts, monitoring, detection, cryptosporidium , microbial risk management, measurement

Progress and Final Reports:

  • Final