2018 Progress Report: Systems Engineering & Analysis for Organotypic Culture Models

EPA Grant Number: R835736C005
Subproject: this is subproject number 005 , established and managed by the Center Director under grant R835736
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Center: Vanderbilt Pittsburgh Resource for Organotypic Models for Predictive Toxicology
Center Director: Hutson, Michael Shane
Title: Systems Engineering & Analysis for Organotypic Culture Models
Investigators: Wikswo, John , Cliffel, David , McLean, John , Shotwell, Matt
Institution: Vanderbilt University
EPA Project Officer: Klieforth, Barbara I
Project Period: December 1, 2014 through November 30, 2018 (Extended to November 30, 2019)
Project Period Covered by this Report: December 1, 2017 through November 30,2018
RFA: Organotypic Culture Models for Predictive Toxicology Center (2013) RFA Text |  Recipients Lists
Research Category: Safer Chemicals , Health , Human Health

Objective:

VPROMPT's Project 5 is designed to ensure physiologically realistic function of each of the mammary, limb, fetal membrane, and liver organotypic culture models (OCMs), operating separately and in combination, and will do so by coordinating the refinement of Integrated Organ Microfluidics (IOM) modules that will provide a common platform architecture.

Progress Summary:

Project 5 continues to deliver standalone pumps, valves, IOM Perfusion Controllers (IOM-PCs, v2.0), and MicroClinical Analyzers (┬ÁCAs, v2.0) as requested by organ developers. A variety of Rotary Planar Peristaltic Micropumps (RPPMs) are in regular use by Projects 1 and 2, and we anticipate their use by Project 3 soon. Project 1 has evaluated the 5-port Rotary Planar Valves (RPVs). Project 5 has made significant progress in developing multi-port RPPMs and RPVs that will soon bring new capabilities to the pumps and valves used by all groups to perfuse OCMs and reduce the cost and increase the ease of use of the hardware. We designed, fabricated, and tested 8-port RPPMs and 2x8-port RPVs, and 6-port versions are being produced for the Project 1 mammary gland OCMs. VPROMPT is benefiting directly from a separately funded development of a new V3.0 motor cartridge, with circular through-plate fluidics, and an openable Puck OCM. The production of IOM fluidic chips has been streamlined with a new, soft-lithography molding process. The V3.0 IOM through-plate fluidic architecture has enabled the development of new and improved IOM fluidic chips, including Ridge, Capacitor, Push-Pull, and LoFlow RPPMs. Significant progress has been made towards full wireless control of both a Triple NEMA-8 IOM PC and a single V3.1 SmartMotor IOM Module, and the latter will be made available to VPROMPT in Q3 2019. We also have worked closely with Project 2 to develop an automated bone-construct limb-segmentation bender.


Journal Articles on this Report : 2 Displayed | Download in RIS Format

Other subproject views: All 32 publications 7 publications in selected types All 7 journal articles
Other center views: All 149 publications 39 publications in selected types All 39 journal articles
Type Citation Sub Project Document Sources
Journal Article Auner AW, Tasneem KM, Markov DA, McCawley LJ, Hutson MS. Chemical-PDMS Binding Kinetics and Implications for Bioavailability in microfluidic Devices. Lab on a Chip 2019;19(5):864-874 R835736 (2018)
R835736C001 (2018)
R835736C005 (2018)
  • Abstract from PubMed
  • Abstract: Lab on a Chip - Abstract HTML
    Exit
  • Journal Article Miller DR, McClain ES, Cliffel DE. Electrochemical Microphysiometry Detects Cellular Glutamate Up-take. Journal of The Electrochemical Society 2018;165(12):G3120-G3124. R835736C005 (2018)
  • Abstract: Journal of the Electrochemical Society - Abstract
    Exit
  • Supplemental Keywords:

    Microfluidic pumps and valves, integrated organ microfluidics

    Relevant Websites:

    Vanderbilt Institute for Integrative Biosystems Research and Education Exit

    Vanderbilt-Pittsburgh Resource for Organotypic Models for Predictive Toxicology (VPROMPT) Exit

    Progress and Final Reports:

    Original Abstract
  • 2015 Progress Report
  • 2016 Progress Report
  • 2017 Progress Report

  • Main Center Abstract and Reports:

    R835736    Vanderbilt Pittsburgh Resource for Organotypic Models for Predictive Toxicology

    Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R835736C001 Mammosphere Bioreactor For Life-Stage Specific Toxicology
    R835736C002 Organotypic Culture Model to Analyze Developmental LimbMalformationsResulting from Toxicant/Teratogen Exposure
    R835736C003 Validating a fetal membrane on a chip model for characterizing reproductive toxicant exposure risks
    R835736C004 Organotypic Liver Model for Predictive Human Toxicology and Metabolism
    R835736C005 Systems Engineering & Analysis for Organotypic Culture Models