Grantee Research Project Results
Publications Details for Grant Number R831041
Investigation of Toxic Raphidophyte Population Dynamics Using Molecular and Physiological Tools
RFA:
Ecology and Oceanography of Harmful Algal Blooms (2002)
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Reference Type | Reference Title | Journal | Author | Citation | Progress Report Year | Document Sources |
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Journal Article | Molecular approaches to the investigation of viable dinoflagellate cysts in natural sediments from estuarine environments. | THE JOURNAL OF EUKARYOTIC MICROBIOLOGY | Coyne KJ, Cary SC | Coyne KJ, Cary SC. Molecular approaches to the investigation of viable dinoflagellate cysts in natural sediments from estuarine environments. The Journal of Eukaryotic Microbiology 2005;52(2):90-94. |
R831041 (2005) R831041 (Final) |
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Journal Article | Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. | LIMNOLOGY AND OCEANOGRAPHY: METHODS | Coyne KJ, Handy SM, Demir E, Whereat EB, Hutchins DA, Portune KJ, Doblin MA, Cary SC | Coyne KJ, Handy SM, Demir E, Whereat EB, Hutchins DA, Portune KJ, Doblin MA, Cary SC. Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. Limnology and Oceanography: Methods 2005;3(Sep):381-391. |
R831041 (2005) R831041 (Final) |
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Journal Article | Distribution of Pfiesteria piscicida cyst populations in sediments of the Delaware Inland Bays, USA. | HARMFUL ALGAE | Coyne KJ, Hare CE, Popels LC, Hutchins DA, Cary SC | Coyne KJ, Hare CE, Popels LC, Hutchins DA, Cary SC. Distribution of Pfiesteria piscicida cyst populations in sediments of the Delaware Inland Bays, USA. Harmful Algae 2006;5(4):363-373. |
R831041 (Final) |
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Journal Article | Assessment of microzooplankton grazing on Heterosigma akashiwo using a species-specific approach combining quantitative real time PCR (QPCR) and dilution methods. | MICROBIAL ECOLOGY | Demir E, Coyne KJ, Doblin MA, Handy SM, Hutchins DA | Demir E, Coyne KJ, Doblin MA, Handy SM, Hutchins DA. Assessment of microzooplankton grazing on Heterosigma akashiwo using a species-specific approach combining quantitative real-time PCR (QPCR) and dilution methods. Microbial Ecology 2008;55(4):583-594. |
R831041 (Final) R833221 (2007) R833221 (Final) |
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Journal Article | Dynamics and short-term survival of toxic cyanobacteria species in ballast water from NOBOB vessels transiting the Great Lakes—implications for HAB invasions. | HARMFUL ALGAE | Doblin MA, Coyne KJ, Rinta-Kanto JM, Wilhelm SW, Dobbs FC | Doblin MA, Coyne KJ, Rinta-Kanto JM, Wilhelm SW, Dobbs FC. Dynamics and short-term survival of toxic cyanobacteria species in ballast water from NOBOB vessels transiting the Great Lakes—implications for HAB invasions. Harmful Algae 2007;6(4):519-530. |
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Journal Article | The biological and biogeochemical consequences of phosphate scavenging onto phytoplankton cell surfaces. | LIMNOLOGY AND OCEANOGRAPHY | Fu F-X, Zhang Y, Leblanc K, Sanudo-Wilhelmy SA, Hutchins DA | Fu F-X, Zhang Y, Leblanc K, Sanudo-Wilhelmy SA, Hutchins DA. The biological and biogeochemical consequences of phosphate scavenging onto phytoplankton cell surfaces. Limnology and Oceanography 2005;50(5):1459-1472. |
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Journal Article | A comparison of future increased CO2 and temperature effects on sympatric Heterosigma akashiwo and Prorocentrum minimum. | HARMFUL ALGAE | Fu F-X, Zhang Y, Warner ME, Feng Y, Sun J, Hutchins DA | Fu F-X, Zhang Y, Warner ME, Feng Y, Sun J, Hutchins DA. A comparison of future increased CO2 and temperature effects on sympatric Heterosigma akashiwo and Prorocentrum minimum. Harmful Algae 2008;7(1):76-90. |
R831041 (Final) R833221 (Final) |
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Journal Article | Evaluating vertical migration behavior of harmful raphidophytes in the Delaware Inland Bays utilizing quantitative real-time PCR. | AQUATIC MICROBIAL ECOLOGY | Handy SM, Coyne KJ, Portune KJ, Demir E, Doblin MA, Hare CE, Cary SC, Hutchins DA | Handy SM, Coyne KJ, Portune KJ, Demir E, Doblin MA, Hare CE, Cary SC, Hutchins DA. Evaluating vertical migration behavior of harmful raphidophytes in the Delaware Inland Bays utilizing quantitative real-time PCR. Aquatic Microbial Ecology 2005;40(2):121-132. |
R831041 (2005) R831041 (Final) |
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Journal Article | Simultaneous enumeration of multiple raphidophyte species by quantitative real-time PCR: capabilities and limitations. | LIMNOLOGY AND OCEANOGRAPHY: METHODS | Handy SM, Hutchins DA, Cary SC, Coyne KJ | Handy SM, Hutchins DA, Cary SC, Coyne KJ. Simultaneous enumeration of multiple raphidophyte species by quantitative real-time PCR: capabilities and limitations. Limnology and Oceanography: Methods 2006;4(Jun):193-204. |
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Journal Article | Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. | HARMFUL ALGAE | Handy SM, Demir E, Hutchins DA, Portune KJ, Whereat EB, Hare CE, Rose JM, Warner M, Farestad M, Cary SC, Coyne KJ | Handy SM, Demir E, Hutchins DA, Portune KJ, Whereat EB, Hare CE, Rose JM, Warner M, Farestad M, Cary SC, Coyne KJ. Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. Harmful Algae 2008;7(5):599-613. |
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Journal Article | Physiological responses during dark survival and recovery in Aureococcus anophagefferens (Pelagophyceae). | JOURNAL OF PHYCOLOGY | Popels LC, MacIntyre HL, Warner ME, Zhang Y, Hutchins DA | Popels LC, MacIntyre HL, Warner ME, Zhang Y, Hutchins DA. Physiological responses during dark survival and recovery in Aureococcus anophagefferens (Pelagophyceae). Journal of Phycology 2007;43(1):32-42. |
R831041 (Final) |
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Journal Article | Fast microzooplankton grazing on fast-growing, low-biomass phytoplankton: a case study in spring in Chesapeake Bay, Delaware Inland Bays and Delaware Bay. | HYDROBIOLOGIA | Sun J, Feng Y, Zhang Y, Hutchins DA | Sun J, Feng Y, Zhang Y, Hutchins DA. Fast microzooplankton grazing on fast-growing, low-biomass phytoplankton: a case study in spring in Chesapeake Bay, Delaware Inland Bays and Delaware Bay. Hydrobiologia 2007;589(1):127-139, DOI10.1007/s10750-007-0730-6. |
R831041 (Final) |
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Journal Article | Bottom-up controls on a mixed-species HAB assemblage: a comparison of sympatric Chattonella subsalsa and Heterosigma akashiwo (Raphidophyceae) isolates from the Delaware Inland Bays, USA. | HARMFUL ALGAE | Zhang Y, Fu F-X, Whereat E, Coyne KJ, Hutchins DA | Zhang Y, Fu F-X, Whereat E, Coyne KJ, Hutchins DA. Bottom-up controls on a mixed-species HAB assemblage: a comparison of sympatric Chattonella subsalsa and Heterosigma akashiwo (Raphidophyceae) isolates from the Delaware Inland Bays, USA. Harmful Algae 2006;5(3):310-320. |
R831041 (Final) |
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Presentation | Mixed raphidophyte blooms in the Delaware Inland Bays, USA: niche selection for a HAB consortium. | None | Coyne KJ, Handy SM, Portune KJ, Demir E, Zhang Y, Doblin M, Whereat E, Cary SC, Hutchins DA | Coyne KJ, Handy SM, Portune KJ, Demir E, Zhang Y, Doblin M, Whereat E, Cary SC, Hutchins DA. Mixed raphidophyte blooms in the Delaware Inland Bays, USA: niche selection for a HAB consortium. Presented at the 11th International Conference on Harmful Algae, Capetown, South Africa, November 15-19, 2004. |
R831041 (2004) R831041 (2005) R831041 (Final) |
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Presentation | Improved accuracy of quantitative real-time PCR of HAB species in environmental water samples using an exogenous DNA internal standard. | None | Coyne KJ, Handy SM, Demir E, Whereat EB, Portune KJ, Hutchins DA, Doblin MA, Cary SC | Coyne KJ, Handy SM, Demir E, Whereat EB, Portune KJ, Hutchins DA, Doblin MA, Cary SC. Improved accuracy of quantitative real-time PCR of HAB species in environmental water samples using an exogenous DNA internal standard. Presented at the Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) Ocean Sciences Meeting on HABs and Eutrophication, Baltimore, MD, March 7-10, 2005. |
R831041 (2005) R831041 (Final) |
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Presentation | Nitrate assimilation in Heterosigma akashiwo: evaluation of nitrate reductase (nr) gene expression in laboratory cultures and in situ populations of H. akashiwo in the Delaware Inland Bays, DE. | None | Coyne KJ, Hutchins DA, Zhang Y, Handy SM, Demir E, Cary SC | Coyne KJ, Hutchins DA, Zhang Y, Handy SM, Demir E, Cary SC. Nitrate assimilation in Heterosigma akashiwo: evaluation of nitrate reductase (nr) gene expression in laboratory cultures and in situ populations of H. akashiwo in the Delaware Inland Bays, DE. Presented at the 3rd Symposium on Harmful Marine Algae in the U.S., Monterey, CA, October 3-6, 2005. |
R831041 (Final) |
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Presentation | Molecular approaches to HAB research: who’s there and what are they doing? | None | Coyne KJ, Doblin MA, Gobler CJ, Hutchins DA, Handy SM, Demir E, Portune KJ, Cary SC | Coyne KJ, Doblin MA, Gobler CJ, Hutchins DA, Handy SM, Demir E, Portune KJ, Cary SC. Molecular approaches to HAB research: who’s there and what are they doing? Presented at the 12th International Conference on Harmful Algae, Copenhagen, Denmark, September 4-8, 2006. |
R831041 (Final) |
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Presentation | Microzooplankton grazing on Heterosigma akashiwo in the Delaware Inland Bays, an application of QRT-PCR technique. | None | Demir E, Coyne KJ, Doblin MA, Hutchins DA | Demir E, Coyne KJ, Doblin MA, Hutchins DA. Microzooplankton grazing on Heterosigma akashiwo in the Delaware Inland Bays, an application of QRT-PCR technique. Presented at the 3rd Symposium on Harmful Marine Algae in the U.S., Monterey, CA, October 3-6, 2005. |
R831041 (Final) |
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Presentation | Description of a novel raphidophyte species and genus from Delaware’s Inland Bays, USA. | None | Demir E, Coyne KJ, Czymmek K, Hutchins DA | Demir E, Coyne KJ, Czymmek K, Hutchins DA. Description of a novel raphidophyte species and genus from Delaware’s Inland Bays, USA. Presented at the 12th International Conference on Harmful Algae, Copenhagen, Denmark, September 4-8, 2006. |
R831041 (Final) |
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Presentation | Global transport and dispersal of HABs in ships' ballast water and recreational vessels. | None | Doblin MA, Coyne KJ, Popels LC, Hutchins DA, Cary SC, Rublee PA, Dobbs FC | Doblin MA, Coyne KJ, Popels LC, Hutchins DA, Cary SC, Rublee PA, Dobbs FC. Global transport and dispersal of HABs in ships' ballast water and recreational vessels. Presented at the 11th International Conference on Harmful Algae, Capetown, South Africa, November 15-19, 2004 (invited). |
R831041 (2004) R831041 (2005) R831041 (Final) |
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Presentation | The role of mesozooplankton grazing in harmful algal bloom dynamics. | None | Doblin MA, Coyne KJ, Demir E, Handy SM, Hutchins DA | Doblin MA, Coyne KJ, Demir E, Handy SM, Hutchins DA. The role of mesozooplankton grazing in harmful algal bloom dynamics. Presented the the 20th Conference of the Australasian Society of Phycologists and Aquatic Biologists, Sydney, Australia, February 13-15, 2006. |
R831041 (Final) |
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Presentation | Rising CO2 and temperature effects on community dynamics of harmful and harmless estuarine algal groups. | None | Fu F-X, Garcia N, Zhang Y, Feng Y, Warner ME, Hutchins DA | Fu F-X, Garcia N, Zhang Y, Feng Y, Warner ME, Hutchins DA. Rising CO2 and temperature effects on community dynamics of harmful and harmless estuarine algal groups. Presented at the American Society of Limnology and Oceanography (ASLO) Science Meeting. Santa Fe, New Mexico, February 5-9, 2007. |
R831041 (Final) |
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Presentation | Harmful dinoflagellate and raphidophyte blooms in the Peconic River Estuary, NY, USA: an assessment of dynamics and nutrient sources promoting blooms. | None | Gobler CJ, Curran PB, Coyne KJ | Gobler CJ, Curran PB, Coyne KJ. Harmful dinoflagellate and raphidophyte blooms in the Peconic River Estuary, NY, USA: an assessment of dynamics and nutrient sources promoting blooms. Presented at the 11th International Conference on Harmful Algae, Capetown, South Africa, November 15-19, 2004. |
R831041 (2004) R831041 (2005) R831041 (Final) |
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Presentation | Investigation of toxic raphidophyte population dynamics using molecular and physiological tools. | None | Handy SM, Coyne KJ , Cary SC , Doblin M, Zhang Y, Whereat E, Portune KJ, Hutchins DA | Handy SM, Coyne KJ , Cary SC , Doblin M, Zhang Y, Whereat E, Portune KJ, Hutchins DA. Investigation of toxic raphidophyte population dynamics using molecular and physiological tools. Presented at the Environmental Protection Agency Science Forum: Healthy Communities and Ecosystems, Washington, DC, June 1-3, 2004. |
R831041 (2004) R831041 (2005) R831041 (Final) |
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Presentation | The vertical migration behavior of a harmful raphidophyte consortium in the Delaware Inland Bays, USA. | None | Handy SM, Coyne KJ , Portune KJ, Demir E , Doblin MA, Cary SC, Hutchins DA | Handy SM, Coyne KJ , Portune KJ, Demir E , Doblin MA, Cary SC, Hutchins DA. The vertical migration behavior of a harmful raphidophyte consortium in the Delaware Inland Bays, USA. Presented at the 11th International Conference on Harmful Algae, Capetown, South Africa, November 15-19, 2004. |
R831041 (2004) R831041 (2005) R831041 (Final) |
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Presentation | The vertical migration behavior of a harmful raphidophyte consortium in the Delaware Inland Bays. | None | Handy SM | Handy SM. The vertical migration behavior of a harmful raphidophyte consortium in the Delaware Inland Bays. Presented at the Center for the Inland Bays Scientific and Technical Advisory Committee Meeting, Lewes, DE, September 10, 2004. |
R831041 (2004) R831041 (2005) R831041 (Final) |
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Presentation | A new application of quantitative real-time PCR: simultaneous enumeration of multiple raphidophyte species by multiprobing and multiplexing. | None | Handy SM, Hutchins DA, Cary SC, Coyne KJ | Handy SM, Hutchins DA, Cary SC, Coyne KJ. A new application of quantitative real-time PCR: simultaneous enumeration of multiple raphidophyte species by multiprobing and multiplexing. Presented at the 3rd Symposium on Harmful Marine Algae in the U.S., Monterey, CA, October 3-6, 2005. |
R831041 (Final) |
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Presentation | Defining ecological niches within a multi-species Raphidophyte HAB consortium. | None | Hutchins DA, Zhang Y, Whereat E, Demir E, Fu F-X, Doblin M, Handy SM, Portune KJ, Cary SC, Coyne KJ | Hutchins DA, Zhang Y, Whereat E, Demir E, Fu F-X, Doblin M, Handy SM, Portune KJ, Cary SC, Coyne KJ. Defining ecological niches within a multi-species Raphidophyte HAB consortium. Presented at the 3rd Symposium on Harmful Marine Algae in the U.S., Monterey, CA, October 3-6, 2005. |
R831041 (Final) |
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Presentation | Global change effects on ocean biology: an experimental approach. | None | Hutchins DA | Hutchins DA. Global change effects on ocean biology: an experimental approach. Presented at the Coalition for National Science Funding Congressional Exhibition and Reception, June 25, 2007 (invited). |
R831041 (Final) |
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Presentation | Investigations of Heterosigma akashiwo (Raphidophyceae) cyst germination in laboratory and field settings using molecular techniques. | None | Portune KJ, Coyne KJ, Handy SM, Hutchins DA, Cary SC | Portune KJ, Coyne KJ, Handy SM, Hutchins DA, Cary SC. Investigations of Heterosigma akashiwo (Raphidophyceae) cyst germination in laboratory and field settings using molecular techniques. Presented at the 3rd Symposium on Harmful Marine Algae in the U.S., Monterey, CA, October 3-6, 2005. |
R831041 (Final) |
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Presentation | Examination of raphidophyte excystment in the Delaware Inland Bays using molecular techniques. | None | Portune K, Coyne KJ, Cary C, Hutchins DA | Portune K, Coyne KJ, Cary C, Hutchins DA. Examination of raphidophyte excystment in the Delaware Inland Bays using molecular techniques. Presented at the 11th International Conference on Harmful Algae, Capetown, South Africa, November 15-19, 2004. |
R831041 (2004) R831041 (2005) R831041 (Final) |
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Presentation | A comparison of nutrient and ecophysiological factors effects on the growth of Chattonella subsalsa and Heterosigma akashiwo (Raphodophyceae) isolated from the Inland Bays, Delaware (USA). | None | Zhang Y, Whereat E, Hutchins DA | Zhang Y, Whereat E, Hutchins DA. A comparison of nutrient and ecophysiological factors effects on the growth of Chattonella subsalsa and Heterosigma akashiwo (Raphodophyceae) isolated from the Inland Bays, Delaware (USA). Presented at the Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) Ocean Sciences Meeting on HABs and Eutrophication, Baltimore, MD, March 7-10, 2005. |
R831041 (Final) |
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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.