Grantee Research Project Results
Final Report: Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
EPA Grant Number: R827015C021Subproject: this is subproject number 021 , established and managed by the Center Director under grant R827015
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: HSRC (1989) - Northeast HSRC
Center Director: Sidhu, Sukh S.
Title: Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
Investigators: McInerney, Michael , Suflita, Joseph , Gieg, Lisa M.
Institution: University of Oklahoma
EPA Project Officer: Aja, Hayley
Project Period: June 1, 2002 through May 31, 2004
RFA: Integrated Petroleum Environmental Consortium (IPEC) (1999) RFA Text | Recipients Lists
Research Category: Hazardous Waste/Remediation , Targeted Research
Objective:
The objective of this research project was to determine the mechanism(s) of action of commercially available, microbial formulations used to treat paraffin deposition in the oil field. There are many conflicting reports by producers on the efficacy of microbial treatments to remedy paraffin deposits, and it is not known why microbial treatments work under some conditions but not others. Knowledge of the mechanism(s) used by microorganisms to remediate paraffin deposits is a critical first step in understanding how the application of microbial treatments for paraffin removal can be optimized in the oil field. Knowing the mechanism(s) of action of these products will allow the independent producer to determine the conditions under which they are likely to succeed and to determine if and when the purchase of microbial commercial paraffin treatments represents a wise expenditure of investment dollars.
Summary/Accomplishments (Outputs/Outcomes):
Introduction
Paraffins are naturally occurring components of crude oils, but often form solids within oil reservoirs and on oil production equipment when oil is harvested from hot subsurface temperatures to the cooler surface environments. Microbial treatment is one approach that can be used to help alleviate this problem, and has been deemed more environmentally friendly, safer to use, and more cost-effective than more traditional thermal or chemical methods of paraffin treatment. There are, however, numerous conflicting reports by producers on the efficacy of microbes to remedy paraffin deposits; it is not known why microbial treatments work under some conditions but not others. Although not scientifically documented, several hypotheses have been proposed as to how microbes may act in oil reservoirs to treat paraffins. These include the “cracking” of long-chain hydrocarbons into shorter chains (a mechanism with no known biological basis), the production of biosurfactants or bioemulsifiers to help mobilize paraffins, or biodegradation of paraffins into oxidized metabolites that may act as “biosolvents.” Thus, in this project, we sought to investigate such proposed mechanism(s) of microbial paraffin treatment in controlled laboratory studies to understand how, why, and when they work. Experiments were conducted in two stages. Stage I experiments consisted of an initial screening of paraffinic oils and microbial products by using a highly sensitive assay that measures changes in the physical properties of oils. Any positive results from Stage I screenings were tested further for possible mechanisms of action in Stage II experiments. Two paraffinic oils were studied in this project, and pertinent results are presented below.
Results of Stage I Experiments
The two oils chosen for study were designated Alaska Oil A and Alaska Oil B. These oils were supplied to a proprietary manufacturer of a widely used commercial microbial treatment product who recommended a unique microbial formulation that should be effective for the treatment of each oil. The Stage I experiments entailed incubating the given oil with artificial brine medium in the absence (negative control) or presence of a proprietary microbial product chosen specifically to treat the oil. Tests were conducted at room temperature (~25°C) and at 60°C either in the presence or absence of oxygen. All test variables were established in triplicate and were incubated for 3 or 7 days. After incubation with slow, end-over-end mixing, oil layers were removed to conduct the wax appearance temperature (WAT) test. This highly sensitive assay uses cross-polarized microscopy to measure the temperature at which paraffin crystals begin to form when a given oil is cooled under controlled conditions. For the purposes of this research, microbial paraffin treatments, which lowered the WAT by a minimum of 5 percent over that of the parallel microbial-free controls, were considered successful for the preliminary screening assays.
Table 1 shows the WAT results obtained during Stage I screening experiments conducted for both Alaska Oils A and B. Statistical analysis (ANOVA test) of all of the WAT data from Alaska Oil A Stage I experiments showed that there was no significant difference in the WAT values for any microbe-amended incubation under any condition relative to the microbe-free (negative) controls. These results suggested that the proprietary microbial product selected to treat this oil was ineffective; therefore, this oil was not studied further.
In contrast, some changes in the WAT values obtained for Alaska Oil B were evident. At 60°C, ANOVA tests showed that there was a significant difference in the WAT values between microbe-free controls and microbe-amended incubations in the presence of oxygen (Table 1). For this oil, aerobic treatment with microbial formulation at 60°C resulted in an 8.5 percent decrease in the WAT relative to microbe-free controls. In the absence of oxygen, a 4.9 percent difference was observed between microbe-free and microbe-amended incubations, barely meeting the 5 percent criterion for successful treatment. When we examined the oil removed from microbe-free tests incubated at 25°C (e.g., negative controls only), we found at least an 11.5 percent difference among the WAT values obtained, well above the 5 percent criterion (data not shown). This variability at 25°C was thought to be due to a phenomenon known as cold seeding in which paraffins drop out of the oil and move into an emulsion layer when the oil is below its cloud point, resulting in irreproducible oil samples. Thus, we concluded that the WAT test could not accurately be performed on oils incubated at room temperature (25°C). Nevertheless, the positive results observed at 60°C with Alaska Oil B led us to establish Stage II experiments to determine the mechanism(s) of action of the microbial formulation, which caused the significant reduction in the WAT values.
Table 1. Wax Appearance Temperature Results for Alaska Oils A and B in State I Experiments
Incubation |
WAT |
ANOVA Test |
Alaska Oil A |
||
25°C aerobic control |
38.1 |
no significant difference |
25°C aerobic + microbes |
37.8 |
|
60°C aerobic control |
39.7 |
no significant difference |
60°C aerobic + microbes |
39.9 |
|
60°C anaerobic + microbes |
42.1 |
|
Alaska Oil B |
||
60°C aerobic control |
28.3 |
significant difference (8.5%) |
60°C aerobic + microbes |
25.9 |
|
60°C anaerobic + microbes |
28.2 |
no significant difference (4.9%) |
60°C anaerobic + microbes |
26.8 |
Results of Stage II Experiments
Stage II experiments with Alaska Oil B were designed to elucidate the mechanism(s) of action of microbial formulations in treating paraffins. Thus, a more detailed mechanism experiment was begun by setting up incubations with Alaska Oil B at 60°C under six different conditions. These included the following amendments: (1) whole formulation (positive control); (2) cells only (are the microbial cells only needed for treatment?); (3) supernatant only (is there some chemical component in the medium associated with the cells that contributes to paraffin treatment?); (4) whole formulation plus chloramphenicol (do cells need to grow for successful treatment?); (5) heat-killed whole formulation (are living cells necessary for treatment?); and (6) no microbes added (negative control). All incubations were conducted in triplicate under both aerobic and anaerobic conditions. Although the initial screening experiments showed a statistically significant decrease only in the WAT under aerobic conditions, anaerobic incubations also were established; the WAT values were near our 5 percent criterion for successful treatment and anaerobic conditions are more representative of oil reservoirs. These Stage II experiments were incubated for approximately 9 weeks to mimic usual successful field treatment times (2 to 3 months). During this time, we assayed for several possible mechanisms of microbial treatment. These included: (1) biosurfactant production (surface tension measurements); (2) bioemulsification activity (assay using incubation supernatant and hexadecane; Trebbau and McInerney, 1996); (3) paraffin “cracking” or biodegradation (oil analysis by high-temperature gas chromatography [HT-GC]); (4) metabolite production (organic solvent extraction and gas chromatography-mass spectrometry [GC-MS]; and (5) effect on wax deposition (cold finger test).
Table 2 shows the surface tension results obtained under aerobic and anaerobic conditions at time 0 and after 9 weeks in all of the above-described incubations. Under aerobic conditions, no significant differences were observed in the negative control or heat-killed incubations. However, statistical analysis (ANOVA tests) showed a small yet statistically significant lowering of the surface tension after the 9-week period in incubations with whole formulation, cells only, supernatant only, and whole formulation in the presence of chloramphenicol. A similar effect also was seen under anaerobic conditions (Table 2). The fact that a decrease in surface tension was observed when either live or heat-killed microbial formulations were added to the oils indicated that some component(s) within the formulation other than the cells was contributing to the observed changes. Similarly, some weak bioemulsification activity also was observed (data not shown), but again, this was evident whether live or heat-killed formulations were incubated with Alaska Oil B.
Table 2. Surface Tension Results for Incubations of Alaska Oil B in Stage II Experiments
Incubation Surface Tension (dynes/cm) | |||
Aerobic |
Time 0 |
9 weeks |
ANOVA |
Negative control |
53.8 |
54.2 |
not significant |
Heat-killed cells |
47.0 |
48.0 |
|
Whole formulation |
53.3 |
47.8 |
significant difference |
Cells only |
55.0 |
50.9 |
|
Supernatant only |
54.4 |
52.7 |
|
Chloramphenicol |
54.9 |
47.7 |
|
Anaerobic |
|||
Negative control |
53.8 |
56.0 |
not significant |
Heat-killed cells |
55.0 |
50.1 |
|
Whole formulation |
72.0 |
53.0 |
significant difference |
Cells only |
55.3 |
53.0 |
|
Supernatant only |
56.8 |
50.8 |
|
Chloramphenicol |
57.5 |
50.0 |
To determine whether paraffin biodegradation was a mechanism of microbial treatment, we examined the oil layers in the incubations for evidence of paraffin decomposition. Oils were sub-sampled from the incubations and analyzed by HT-GC to determine whether decreases in paraffin concentrations were evident or whether the paraffin profile shifted from one of higher molecular weight to lower molecular weight alkanes. Prior to HT-GC analysis, oil samples were amended with C24D50 as an internal standard for quantification. Table 3 shows the oil-to-internal standard peak area ratio of the paraffins quantified (C10 to C40) for aerobic and select anaerobic incubations. Using this method of quantification, the oil-to-internal standard peak area ratio should decrease if a reduction in the paraffins occurred. As can be seen in Table 3, the oil-to-internal standard peak area ratios were remarkably similar in all of the Stage II incubations, including the microbe-free controls, indicating that no paraffin biodegradation occurred. In similar fashion, known putative paraffin metabolites (such as long-chain fatty acids or alcohols that would be produced under aerobic conditions, or alkylsuccinates and branched fatty acids that would be produced under anaerobic conditions) were not detected using organic extraction and GC-MS analysis in any of the Stage II incubations.
Table 3. Oil-to-Internal Standard Ratio of Alaska Oil B Analysis in Stage II Experiments
Incubation |
Oil-to-Internal Standard Ratio |
Aerobic |
|
Negative control |
37.4 +/- 4.3 |
Heat-killed cells |
38.4 +/- 1.5 |
Whole formulation |
38.3 +/- 2.3 |
Cells only |
38.5 +/- 2.9 |
Supernatant only |
35.5 +/- 1.5 |
Chloramphenicol |
42.2 +/- 5.9 |
Anaerobic |
|
Negative control |
48.5 +/- 2.2 |
Whole formulation |
46.4 +/- 2.1 |
In all previous experiments, a minimal salts brine medium was used for incubations of the microbial formulations, and oil was supplied as the sole carbon and energy source. In field application of the microbial formulations, a nutrient solution often is added to help stimulate the in situ activity of the microbes added. Thus, another series of experiments was conducted to determine the effects of adding a commercial nutrient solution to the incubations. To this end, test bottles containing microbial formulation plus nutrients were incubated alongside those containing only the microbial formulation. Incubations were conducted at 60°C under aerobic and anaerobic conditions. Appropriate microbe-free controls also were established. After a 12-week incubation period, supernatants were analyzed for changes in surface tension and oils were assayed for paraffin alterations using HT-GC. As with earlier experiments, HT-GC analyses revealed no difference among oil-to-internal standard ratios in any of the microbe-free or microbe-amended incubations, indicating that no biodegradation or paraffin “cracking’ occurred. Further, a statistically significant decrease in surface tension was observed only in the absence of nutrients under aerobic conditions, mimicking previous results.
In addition to the above biology-based assays, we also conducted cold-finger tests to assess whether microbial formulations were functioning in a more physical capacity to prevent wax deposition as opposed to treating wax once it has already been deposited. The cold-finger assays revealed that microbial formulations did not prevent wax deposition (data not shown).
Overall Summary
We had little success in determining an overwhelming mechanism of action of microbial paraffin treatment using commercial formulations because all measurements made showed either no significant differences or only weak significant differences in microbe-amended versus microbe-free incubations. Initial screening experiments showed a significant reduction in the WAT in one of the oils tested (Alaska Oil B), which prompted more detailed mechanism studies. Paraffin biodegradation or “cracking,” or wax deposition prevention were not mechanisms involved in microbial paraffin treatment. Small yet statistically significant changes in surface tension and emulsification assays suggested that some component(s) of the microbial formulations had surfactant-like or emulsifying activity. These latter phenomena were not necessarily cell-associated, because incubations with heat-killed cells or “supernatant only” fractions of the microbial formulations showed surface tension reduction. The addition of a commercial nutrient solution did not improve the microbial treatment of paraffinic oils in our controlled laboratory studies.
Journal Articles:
No journal articles submitted with this report: View all 1 publications for this subprojectSupplemental Keywords:
petroleum, paraffin, microbial treatment,, RFA, Scientific Discipline, INTERNATIONAL COOPERATION, Waste, TREATMENT/CONTROL, Sustainable Industry/Business, Environmental Chemistry, Sustainable Environment, Technology, Technology for Sustainable Environment, Analytical Chemistry, Economics and Business, Technical Assistance, Bioremediation, Ecological Risk Assessment, Ecology and Ecosystems, pollution prevention, Environmental Engineering, chemical waste, clean technologies, cleaner production, microbial degradation, hazardous emissions, petrochemicals, oil production, biodegradation, oil production tank bottoms, hazardous waste, pollution control, IPEC, anaerobic biodegradation, paraffin deposition, innovative technology, technology transfer, technology researchRelevant Websites:
Progress and Final Reports:
Original AbstractMain Center Abstract and Reports:
R827015 HSRC (1989) - Northeast HSRC Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R827015C001 Evaluation of Road Base Material Derived from Tank Bottom Sludges
R827015C002 Passive Sampling Devices (PSDs) for Bioavailability Screening of Soils Containing Petrochemicals
R827015C003 Demonstration of a Subsurface Drainage System for the Remediation of Brine-Impacted Soil
R827015C004 Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C005 Microflora Involved in Phytoremediation of Polyaromatic Hydrocarbons
R827015C006 Microbial Treatment of Naturally Occurring Radioactive Material (NORM)
R827015C007 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C008 The Use of Nitrate for the Control of Sulfide Formation in Oklahoma Oil Fields
R827015C009 Surfactant-Enhanced Treatment of Oil-Contaminated Soils and Oil-Based Drill Cuttings
R827015C010 Novel Materials for Facile Separation of Petroleum Products from Aqueous Mixtures Via Magnetic Filtration
R827015C011 Development of Relevant Ecological Screening Criteria (RESC) for Petroleum Hydrocarbon-Contaminated Exploration and Production Sites
R827015C012 Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C013 New Process for Plugging Abandoned Wells
R827015C014 Enhancement of Microbial Sulfate Reduction for the Remediation of Hydrocarbon Contaminated Aquifers - A Laboratory and Field Scale Demonstration
R827015C015 Locating Oil-Water Interfaces in Process Vessels
R827015C016 Remediation of Brine Spills with Hay
R827015C017 Continuation of an Investigation into the Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C018 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C019 Biodegradation of Petroleum Hydrocarbons in Salt-Impacted Soil by Native Halophiles or Halotolerants and Strategies for Enhanced Degradation
R827015C020 Anaerobic Intrinsic Bioremediation of MTBE
R827015C021 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R827015C022 A Continuation: Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C023 Data for Design of Vapor Recovery Units for Crude Oil Stock Tank Emissions
R827015C024 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells
R827015C025 A Continuation of Remediation of Brine Spills with Hay
R827015C026 Identifying the Signature of the Natural Attenuation of MTBE in Goundwater Using Molecular Methods and "Bug Traps"
R827015C027 Identifying the Signature of Natural Attenuation in the Microbial
Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and
"Bug Traps"
R827015C028 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R827015C030 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R827015C031 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R827015C032 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633 Integrated Petroleum Environmental Consortium (IPEC)
R830633C001 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells (Phase II)
R830633C002 A Continuation of Remediation of Brine Spills with Hay
R830633C003 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R830633C004 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R830633C005 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633C006 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R830633C007 Identifying the Signature of the Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and “Bug Traps”
R830633C008 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R830633C009 Use of Earthworms to Accelerate the Restoration of Oil and Brine Impacted Sites
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.
Project Research Results
Main Center: R827015
120 publications for this center
16 journal articles for this center