Grantee Research Project Results
Electrochemical Enzyme Immunoassay to Study Alternative Drinking Water Treatment
EPA Contract Number: 68D00031Title: Electrochemical Enzyme Immunoassay to Study Alternative Drinking Water Treatment
Investigators: O'Daly, John P.
Small Business: Enzyme Technology Research Group Inc.
EPA Contact: Richards, April
Phase: I
Project Period: August 1, 1990 through February 1, 1991
Project Amount: $49,978
RFA: Small Business Innovation Research (SBIR) - Phase I (1991) RFA Text | Recipients Lists
Research Category: SBIR - Water and Wastewater , Watersheds , Small Business Innovation Research (SBIR)
Description:
Alternatives to chlorine disinfection in drinking water treat- ment are being investigated. Both current and new methods of disinfection may cause reactions with organic contami- nants in the water and lead to dangerous by-products. There is a need for measurement devices that provide on-site, real- time measurement of by-products to assist in the develop- ment of alternate water treatment methods. For this purpose, the researchers propose to evaluate and develop a new biosensor design that combines advantages of immunoas- say and electrochemical response. The specific objective of the Phase I work is to prove the principle of electrochemical enzyme immunosensors to detect organic by-product for- mation in water. The proposed biosensor consists of three major elements; an electrical conducting layer having im- mobilized enzyme, a gel layer containing polyclonal or monoclonal antibody and other necessary reagents, and the electronic components used in the readout of the signal. The expected result is an immunoelectrode for measuring or- ganics in water, based on the principal of coupling the immunochemical reaction to the electrode response using an electrochemically active mediator. The three researchers who submitted this proposal have collaborated successfully to develop prototype enzyme-based biosensor technology which will be a crucial component for the research and development described in this proposal. Practical electro- chemical sensors that employ immunochemical detection will have broad commercial viability for applications in the fields of clinical chemistry, veterinary medicine, agriculture, environmental monitoring, and pollution control.Supplemental Keywords:
Sustainable Industry/Business, RFA, Scientific Discipline, Ecosystem Protection/Environmental Exposure & Risk, Water, Toxics, Technology for Sustainable Environment, Chemical Engineering, Engineering, Chemistry, Sustainable Environment, Environmental Chemistry, Engineering, Chemistry, & Physics, Analytical Chemistry, Monitoring/Modeling, EPCRA, Biochemistry, Drinking Water, Environmental Engineering, biosensors, electrochemical enzyme, electrochemical, chlorine-based disinfection, treatment, Chlorine, chlorinated by products, biosensor, electrochemical enzymes, measurement, chlorine disinfection, electrically driven enzymes, disinfection byproducts (DBP), electrochemical techniques, disinfection by-products, real-time monitoring, electrochemical enzyme, immunoassay, enzymes, biosensing, immunological assay, chlorinated DBPs, real time, alternative disinfection methods, chlorine disinfection alternatives, DBP risk managementProgress and Final Reports:
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.