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Grantee Research Project Results

Final Report: The NODE biosensor: real-time contaminant detection in stormwater

EPA Contract Number: 68HERD19C0025
Title: The NODE biosensor: real-time contaminant detection in stormwater
Investigators: Altizer, Megan
Small Business: Enoveo USA, LLC.
EPA Contact: Richards, April
Phase: I
Project Period: May 1, 2019 through October 31, 2019
Project Amount: $100,000
RFA: Small Business Innovation Research (SBIR) - Phase I (2019) RFA Text |  Recipients Lists
Research Category: SBIR - Wastewater, Stormwater, and Water Reuse , Small Business Innovation Research (SBIR) , Small Business Innovation Research (SBIR): Phase 1 (2019)

Description:

Stormwater management is a growing challenge across the country. Water quality standards are becoming increasingly strict as new regulations are enacted. Meanwhile, changing land use and greater urbanization make meeting these standards even more difficult. Currently, stormwater samples are collected in the field and analyzed in the lab, a process which is time-consuming, costly, delayed and provides poor data resolution. Alternatively, in situ biosensors can offer real-time, online, continuous monitoring of stormwater quality by measuring water quality in a less labor-intensive, more efficient, and sustainable manner. These sensors have been pilot-scale tested in wastewater and groundwater applications. The objective of this Phase I grant was to further develop the current design for stormwater applications, validate it in a real environment, and explore its sensitivity to a broader range of contaminants. 

Summary/Accomplishments (Outputs/Outcomes):

Phase I efforts show that the modified biosensor can support an active biofilm in real world applications for at least three months. This sensor proved robust and able to withstand difficult and dynamic environmental changes associated with the rainy season, while maintaining responsiveness to flow and water quality fluctuations. Further tests showed responsiveness to a wider variety of contaminants of concern, including eutrophication-associated nutrients.

Conclusions:

Based on the results of Phase I, applications of the biosensor for stormwater applications are considered feasible.  Additional work is recommended to model the relationship between flow and contaminant concentrations for increased specificity and to explore other contaminants of interest like metals, pesticides, and fluorinated compounds.
 
In Phase II, Microbiome Engineering (formerly ENOVEO USA) LLC will rigorously characterize the modified anode design created in Phase I to better describe dynamic environmental conditions. We will pilot test the biosensor in a variety of real-world environments, across industries and regions. With continuous data collection online and in real time and proven responsiveness under long term exposure to low substrate conditions, Microbiome Engineering expects the sensor to have further applications in drinking water.

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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Last updated April 28, 2023
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