Design and Synthesis of CO2-Soluble Affinity Ligands for Use in CO2 Extraction of Proteins

EPA Grant Number: R824730
Title: Design and Synthesis of CO2-Soluble Affinity Ligands for Use in CO2 Extraction of Proteins
Investigators: Beckman, Eric J. , Russell, Alan J.
Institution: University of Pittsburgh
EPA Project Officer: Klieforth, Barbara I
Project Period: October 1, 1995 through September 30, 1998
Project Amount: $150,000
RFA: Technology for a Sustainable Environment (1995) Recipients Lists
Research Category: Nanotechnology , Sustainability , Pollution Prevention/Sustainable Development


This proposal involves the design of highly CO2-soluble ligands for the extraction of molecules of biological origin. Carbon dioxide is a relatively benign solvent, and one where recovery of solutes from solution is easily accomplished via depressurization. Unfortunately, CO2 is a low dielectric fluid, and thus will not solubilize polar/hydrophilic molecules such as are commonly generated in biotechnical processes. Further, CO2 is a poor solvent for many commonly used extractant molecules, such as sufactants and chelating agents. Thus, this proposal focuses on the design of extractants/ligands particularly for use in CO2, to allow extension of CO2 to processes where its use is currently untenable. This is a jointly funded NSF/EPA project. In the EPA part of the project, we are focusing on the design of CO2-soluble chiral mobile phase agents (CMPA's) for the separation of enantiomers of ibuprofen and naproxen. A well-designed CMPA interacts specifically with one enantiomer of a racemic mixture, and thus allows separation, often times via salt formation and thus precipitation. Our goal is to combine the selectivity of a CMPA with the pressure-sensitive phase behavior inherent to CO2-based solutions to increase selectivity in a CMPA-moderated separation.

Publications and Presentations:

Publications have been submitted on this project: View all 11 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 2 journal articles for this project

Supplemental Keywords:

Carbon-dioxide-soluble affinity ligands; biological extraction; separation of enantiomer; racemic target molecules; chiral mobile phase ligands; carbon-dioxide-philic tails; fluoroalkyl and fluoroether., RFA, Scientific Discipline, Sustainable Industry/Business, Sustainable Environment, Environmental Chemistry, cleaner production/pollution prevention, Technology for Sustainable Environment, Ecological Risk Assessment, bilogical extraction, cleaner production, environmentally benign solvents, depressurization, carbon dioxide extraction, proteins, ligands, chiral mobile phase agents

Progress and Final Reports:

  • 1996
  • 1997
  • Final Report