Microarray System for Contaminated Water Analysis

EPA Grant Number: X832541C001
Subproject: this is subproject number 001 , established and managed by the Center Director under grant X832541
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Center: Center for Environmental and Energy Research (CEER)
Center Director: Earl, David A.
Title: Microarray System for Contaminated Water Analysis
Investigators: DeRosa, Rebecca , Cardinale, Jean , Thatcher, Ryan
Institution: Alfred University
Current Institution: Inamori School of Engineering , Alfred University
EPA Project Officer: Lasat, Mitch
Project Period: September 1, 2006 through August 31, 2007
RFA: Targeted Research Center (2006) Recipients Lists
Research Category: Targeted Research

Objective:

Our previous study, on a functionalized glass surface to covalently immobilize antibodies, was conducted to produce a more efficient means of water contamination analysis. The current methods used to detect organisms in water require up to 4 days to produce results that are indicative of only a small number of potential contaminates. Enzyme linked immunosorbent assays (ELISA) can use immunological methods to detect a wide range of biomolecules in less time than the current methods. However, traditional ELISAs produce a significant amount of solid waste and require many high-purity antibodies. The costs incurred and biologically- contaminated waste produced by ELISAs have led to the production of a smaller alternative called a microarray. Microarrays use immobilized biomolecules (proteins, lipids, antibodies, and carbohydrates) to detect conjugate biomolecules in solution. Multiple types of biomolecules can be printed onto a single solid glass or polymer substrate to detect multiple conjugate molecules in a single test. Our previous work focused on modification of a glass substrate to increase the surface area available for immobilization and the means by which antibodies were bound to the surface. Covalent immobilization of the antibodies instead of hydrophobic binding is one method that can optimize the binding concentration of antibodies onto the substrate. Our results indicated that surface modifications we made to glass substrates successfully immobilized antibodies. However, the immobilized antibodies denatured, and did not retain their functionality after the immobilization process. Stabilization of the antibodies structure is a potential solution to prevent the denaturing caused during immobilization.

Publications and Presentations:

Publications have been submitted on this subproject: View all 1 publications for this subprojectView all 10 publications for this center

Journal Articles:

Journal Articles have been submitted on this subproject: View all 1 journal articles for this subprojectView all 2 journal articles for this center

Supplemental Keywords:

ELISA, lab on a chip, water contaminants, glass surface modification, protein stabilization, gluteraldehyde, trehalose,

Progress and Final Reports:

  • Final Report

  • Main Center Abstract and Reports:

    X832541    Center for Environmental and Energy Research (CEER)

    Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    X832541C001 Microarray System for Contaminated Water Analysis
    X832541C003 The Fining Behavior of Selectively Batched Commercial Glasses
    X832541C004 The Use of Fly Ash in the Production of SiAlON based Structural Ceramics
    X832541C005 Separation and Purification of Hydrogen From Mixed Gas Streams Using Hollow Glass Microspheres
    X832541C006 Magnesium Rich Coatings for Corrosion Control of Reactive Metal Alloys
    X832541C008 Tunneled Titanate Photocatalysts for Environmental Remediation and Hydrogen Generation
    X832541C009 Material and Environmental Sustainability in Ceramic Processing
    X832541C010 Robust, Spectrally Selective Ceramic Coatings for Recycled Solar Power Tubes
    X832541C011 Recycling of Silicon-Wafers Production Wastes to SiAlON Based Ceramics with Improved Mechanical Properties
    X832541C012 Emissions Reduction of Commercial Glassmaking Using Selective Batching