2006 Progress Report: San Joaquin Valley Aerosol Health Effects Research Center (SAHERC)

EPA Grant Number: R832414
Center: San Joaquin Valley Aerosol Health Effects Research Center (SAHERC)
Center Director: Wexler, Anthony S.
Title: San Joaquin Valley Aerosol Health Effects Research Center (SAHERC)
Investigators: Wexler, Anthony S. , Fanucchi, Michelle V. , Pinkerton, Kent E. , Wilson, Dennis
Current Investigators: Wexler, Anthony S. , Pinkerton, Kent E.
Institution: University of California - Davis
EPA Project Officer: Chung, Serena
Project Period: October 1, 2005 through September 30, 2010 (Extended to September 30, 2011)
Project Period Covered by this Report: October 1, 2005 through September 30, 2006
Project Amount: $7,999,767
RFA: Particulate Matter Research Centers (2004) RFA Text |  Recipients Lists
Research Category: Health Effects , Particulate Matter , Air

Objective:

This research investigates the mechanistic links between ambient particles and health effects. This objective includes two goals: (1) understanding the metabolic response of tissue and organs when they are exposed to particulate pollutants; and (2) understanding the characteristics of the particulate pollutants and their gaseous co-pollutants that elicit these responses. Five projects are underway at the Center. The projects investigate the metabolic response to pollutant exposure in pulmonary and cardiovascular tissues, whole animal effects of exposure, transport of particles from the airways to other tissues, and the effects of particles and gases on lung development in juveniles.

Both field and laboratory studies are being performed. The field studies take place in the San Joaquin Valley of California, one of the worst violators of the National Ambient Air Quality Standards for particulate matter. UC Davis is located at the northern end of the San Joaquin Valley which facilitates this work. The laboratory studies at UC Davis examine the effects of particles from ambient sources and laboratory-generated particles with carefully controlled properties that model ambient ones or those from dominant sources. The research team is composed of physiologists, toxicologists, chemists, and engineers who already collaborate on air pollution studies in the UC Davis Air Quality Research Center. The SAHERC researchers are investigating a complementary set of hypotheses related to the mechanisms and loci of action that cause acute and chronic responses in normal animal models of children and adults and those made susceptible by exposure to particles and ozone during development.

The San Joaquin Valley (SJV) is an ideal location to study the nexus of air pollutants and their health effects because its residents are exposed to some of the worst particulate air pollution in the country, community health effects are evident. Air-quality–related health indicators reflect the severity of the region’s air quality problems; for example, asthma and cardiovascular disease rates are some of the highest in the country. The San Joaquin Valley routinely experiences some of the highest fine airborne particulate matter concentrations in the U.S., with fine and ultrafine particle concentrations that rival those found in Los Angeles. The American Lung Association reports that three of the four most polluted American cities are located in the SJV. Many of the air pollution source categories in the SJV are the same as in the rest of the country: transportation, wood smoke, refineries, and agriculture. The Valley also encompasses sharp gradients—concentrations are higher in the south-central portion of the valley and significantly lower in the foothills and further north; there is a seasonal variation in the concentrations as well.

The AQRC at UC Davis has research investigations underway ranging from biological effects of air pollution to global climate research. The scientists on the AQRC team who are working on this project investigate pulmonary and cardiovascular biology and aerosol and atmospheric science, working together to examine urban and regional smog and its health effects. UC Davis has one of the largest concentrations of university faculty with air pollution expertise at any one campus in the U.S. with over 70 faculty members on campus involved in air quality research and affiliated with the AQRC at UC Davis.

Progress Summary:

This is a general progress report for the SAHERC. The progress for the specific research projects conducted by the Center is reported separately.

All studies in the SAHERC are being undertaken within the primary theme of ‘ambient particulate matter and resulting health effects in the San Joaquin Valley.’ Below is a chart outlining the center’s five projects. All five research projects are complementary and focus on the role of particulates in pulmonary and cardiovascular health effects. The research progress for these studies is summarized below in a paragraph for each project.

Image 1.

Project 1—Pulmonary Metabolic Response

Principal Investigator: Michelle Fanucchi
Co-Investigators: Charles Plopper, Alan Buckpitt

First, to evaluate the acute effects of particulate exposure in rats, validated the use of a dry powder insufflator as a consistent method for acute, intrapulmonary delivery of 1 to 5 mg of particulates evenly throughout the airways. Second, we validated a strategy to systematically define the impact that individual components of particulate matter have on the cytotoxicity of airway epithelium. We found that carbon particles not coated with 1-nitronaphthalene did not cause any appreciable cellular injury to airway epithelium and that the majority of the particles were cleared from the airways within 2 hours. However, particles coated with 1-nitronaphthalene caused focal areas of exfoliation and cellular injuries in all airways evaluated (proximal, mid-level and distal airways) and were not completely cleared from the airways.

Project 2—Endothelial Cell Responses to PM—In Vitro and In Vivo

Principal Investigator: Dennis Wilson
Co-Investigator: John Rutledge

We concentrated on signaling responses associated with oxidant stress using laboratory generated particles as well as historically archived (NIST) environmental particles until ambient particulate matter from the San Joaquin Valley are collected. We also performed several experiments evaluating culture exposure methods that will best recapitulate expectations of exposure conditions in vivo.

Project 3—Inhalation Exposure Assessment of San Joaquin Valley Aerosol

Principal Investigator: Kent Pinkerton
Co-Investigators: Mike Kleeman, Ann Bonham, John Rutledge

We constructed the prototype insert for holding individual mice in the exposure chamber and conducted preliminary CAPs exposure where 24 hr ECG signals were recorded for HRV analysis using an implanted ECG telemetry system. We are currently analyzing these data. Samples of airborne particulate matter were collected during the week of October 31 – November 4, 2005 and November 7 – November 11, 2005 each day from 9am – 3pm to coincide with animal exposure periods. The PM1.8 mass and Ultrafine (PM0.1) concentrations were significantly lower than concentrations experienced during typical air pollution events in the SJV. Organic carbon, elemental carbon, and water soluble ions (sulfate, nitrate, etc) made up only a very small fraction of the particle mass collected during sampling. This contrasts sharply with previous samples collected during the winter in the SJV where carbon and water soluble ions made up the majority of the particle mass. Elemental analysis of these samples will be conducted in the following weeks.

Project 4—Transport Mechanisms and Systemic Fate of Inspired Ultrafine Particles

Principal Investigator: Dennis Wilson
Co-Investigators: Angelique Louie, Michelle Fanucchi, Ian Kennedy, Charles Plopper, Alan Buckpitt

Nanoparticles can be successfully labeled with copper by pre-incubation of copper with p-SCN-Bz-DOTA followed by immediate conjugation of the p-SCN-Bz-Cu-DOTA to aminated polystyrene beads in a tetramethylammonium phosphate buffer. Whole animal imaging studies in the first reporting period were limited by the challenges of particle conjugation. Given the difficulties in creating the Cu64 conjugates, we also evaluated the potential for using intravital fluorescence as an imaging modality in these studies. Results of this study showed significant background autofluorescence in the whole animal but that fluorescent signal could be detected in dissected tissues. Unfortunately this initial experiment resulted in intraesophageal instillation but did offer proof of concept for potential future studies using florescence labeled particles in isolated organs.

Project 5—Architecture Development and Particle Deposition

Investigators: Anthony Wexler and Charles Plopper

Two current activities are highlighted, acquisition of CT image data, and second, characterization of pulmonary architecture. First, a custom designed micro CT scanner was used to obtain voxel based images of lung casts. The 3D CT data set was reconstructed from 1000 projections with a custom developed cone beam reconstruction program using Feldkamp-Davis-Kress (FDK) algorithm. Second, we developed a new algorithm to quantify the branching structure of the pulmonary tree. This algorithm was able to successfully analyze airway radii as small as 2 voxels. The algorithm was validated by error analysis and comparison of statistical results obtained by comparing our technique with previous anatomy studies of rat lung (Phillips 1995, Sera 2003).

Future Activities:

Planned activities for the next reporting period are described by project below.

Project 1—Pulmonary Metabolic Response

Since, the initial experiments did not indicate a strong dose-response relationship between the amount of 1-nitronaphthalene coated on the particles and the amount of injury present in the airways, we will reduce the amount of 1-nitronapthalene per particle. Also, we will investigate whether it is the concentration of the PAH on individual particles or the total PAH concentration administered that is important in particle toxicity, and whether the type of core particle affects the toxicity. Future studies will utilize flame-generated soot doped with 1-nitronaphthalene. In addition, ambient particles from urban and rural sites in the San Joaquin Valley will be assessed for their cytotoxicity. The samples with the greatest cytotoxicity will be analyzed for PAH concentrations. The laboratory generated particles will then be adjusted to contain PAH’s relevant to those found in San Joaquin Valley samples.

Project 2—Endothelial Cell Responses to PMIn Vitro and In Vivo

In the next project year we plan to apply our ROS signaling assays to evaluate responses to SJV derived PM in cultured human aortic and pulmonary artery endothelium. We will evaluate potential approaches to ROS detection with ESR as an alternative to dye based assays. We plan to complete the proposed evaluation of TGFb family signaling in response to PM as well as the proposed evaluation of PM effects on endothelial barrier function.

Project 3—Inhalation Exposure Assessment of San Joaquin Valley Aerosol

For the next reporting period, our plan is to (1) continue with exposures in the late summer season (i.e., September); (2) conduct CAPs experiments in our designated urban site of the San Joaquin Valley (Fresno); (3) analyze the HRV, particle concentration and composition, stress test results; and (4) conduct patch-clamping analysis of identified cardiac vagal neurons.

Project 4—Transport Mechanisms and Systemic Fate of Inspired Ultrafine Particles

In the next year we plan to focus on the blood kinetics of indium labeled particles while continuing development of our PET imaging studies. Key to the latter will be consistent labeling of synthetic ultrafine particles with Cu60 as this has been the most significant challenge in our initial year. We are also exploring the option of Xenogen based fluorescence imaging as a developmental tool that does not require the use of radiochemicals. We will continue our transcellular transport studies in cultured endothelial cells using fluorescence tagged particles in concert with dual labeling for caveolar proteins.

Project 5—Architecture Development and Particle Deposition

We will continue to improve the algorithm so as to clearly distinguish erroneous data from reliable results. In addition we will try various optimization subroutines to reduce computer analysis time. We will characterize normal architecture geometric parameters and their normal variations in adult rats before commencing with ozone and ozone+PM exposures.


Journal Articles: 64 Displayed | Download in RIS Format

Other center views: All 128 publications 71 publications in selected types All 64 journal articles
Type Citation Sub Project Document Sources
Journal Article Aung HH, Lame MW, Gohil K, He G, Denison MS, Rutledge JC, Wilson DW. Comparative gene responses to collected ambient particles in vitro:endothelial responses. Physiological Genomics 2011;43(15):917-929. R832414 (Final)
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  • Journal Article Bein KJ, Zhao Y, Wexler AS. Conditional sampling for source-oriented toxicological studies using a single particle mass spectrometer. Environmental Science & Technology 2009;43(24):9445-9452. R832414 (Final)
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  • Journal Article Bein KJ, Wexler AS. A high-efficiency, low-bias method for extracting particulate matter from filter and impactor substrates. Atmospheric Environment 2014;90:87-95. R832414 (Final)
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  • Journal Article Bein KJ, Zhao Y, Wexler AS. Retrospective source attribution for source-oriented sampling. Atmospheric Environment 2015;119:228-239. R832414 (Final)
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  • Journal Article Breysse PN, Delfino RJ, Dominici F, Elder ACP, Frampton MW, Froines JR, Geyh AS, Godleski JJ, Gold DR, Hopke PK, Koutrakis P, Li N, Oberdorster G, Pinkerton KE, Samet JM, Utell MJ, Wexler AS. US EPA particulate matter research centers: summary of research results for 2005–2011. Air Quality, Atmosphere & Health 2013;6(2):333-355. R832414 (Final)
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  • Journal Article Chan JKW, Fanucchi MV, Anderson DS, Abid AD, Wallis CD, Dickinson DA, Kumfer BM, Kennedy IM, Wexler AS, Van Winkle LS. Susceptibility to inhaled flame-generated ultrafine soot in neonatal and adult rat lungs. Toxicological Sciences 2011;124(2):472-486. R832414 (Final)
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  • Journal Article Chan JKW, Charrier JG, Kodani SD, Vogel CF, Kado SY, Anderson DS, Anastasio C, Van Winkle LS. Combustion-derived flame generated ultrafine soot generates reactive oxygen species and activates Nrf2 antioxidants differently in neonatal and adult rat lungs. Particle and Fibre Toxicology 2013;10:34. R832414 (Final)
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  • Journal Article Chan JKW, Kodani SD, Charrier JG, Morin D, Edwards PC, Anderson DS, Anastasio C, Van Winkle LS. Age-specific effects on rat lung glutathione and antioxidant enzymes after inhaling ultrafine soot. American Journal of Respiratory Cell and Molecular Biology 2013;48(1):114-124. R832414 (Final)
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  • Journal Article Chen C-Y, Chow D, Chiamvimonvat N, Glatter KA, Li N, He Y, Pinkerton KE, Bonham AC. Short-term secondhand smoke exposure decreases heart rate variability and increases arrhythmia susceptibility in mice. American Journal of Physiology-Heart and Circulatory Physiology 2008;295(2):H632-H639. R832414 (Final)
    R831918 (Final)
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  • Journal Article Day KC, Plopper CG, Fanucchi MV. Age-specific pulmonary cytochrome P-450 3A1 expression in postnatal and adult rats. American Journal of Physiology-Lung Cellular and Molecular Physiology 2006;291(1):L75-L83. R832414 (2009)
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  • Journal Article den Hartigh LJ, Lame MW, Ham W, Kleeman MJ, Tablin F, Wilson DW. Endotoxin and polycyclic aromatic hydrocarbons in ambient fine particulate matter from Fresno, California initiate human monocyte inflammatory responses mediated by reactive oxygen species.Toxicology In Vitro 2010;24(7):1993-2002. R832414 (2010)
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  • Journal Article Donaldson K, Borm PJA, Oberdorster G, Pinkerton KE, Stone V, Tran CL. Concordance between in vitro and in vivo dosimetry in the proinflammatory effects of low-toxicity, low-solubility particles: the key role of the proximal alveolar region. Inhalation Toxicology 2008;20(1):53-62. R832414 (Final)
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  • Journal Article Enright HA, Bratt JM, Bluhm AP, Kenyon NJ, Louie AY. Tracking retention and transport of ultrafine polystyrene in an asthmatic mouse model using positron emission tomography. Experimental Lung Research 2013;39(7):304-313. R832414 (Final)
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  • Journal Article Ge X, Zhang Q, Sun Y, Ruehl CR, Setyan A. Effect of aqueous-phase processing on aerosol chemistry and size distributions in Fresno, California, during wintertime. Environmental Chemistry 2012;9(3):221-235. R832414 (Final)
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  • Journal Article Ge X, Setyan A, Sun Y, Zhang Q. Primary and secondary organic aerosols in Fresno, California during wintertime: results from high resolution aerosol mass spectrometry. Journal of Geophysical Research-Atmospheres 2012;117:15. R832414 (Final)
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  • Journal Article Gojova A, Lee JT, Jung HS, Guo B, Barakat AI, Kennedy IM. Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells. Inhalation Toxicology 2009;21(Suppl 1):123-130. R832414 (Final)
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  • Journal Article Greeley MA, Van Winkle LS, Edwards PC, Plopper CG. Airway trefoil factor expression during naphthalene injury and repair. Toxicological Sciences 2010;113(2):453-467. R832414 (Final)
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  • Journal Article Ham WA, Herner JD, Green PG, Kleeman MJ. Size distribution of health-relevant trace elements in airborne particulate matter during a severe winter stagnation event: implications for epidemiology and inhalation exposure studies. Aerosol Science and Technology 2010;44(9):753-765. R832414 (2010)
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  • Journal Article Ham WA, Kleeman MJ. Size-resolved source apportionment of carbonaceous particulate matter in urban and rural sites in central California. Atmospheric Environment 2011;45(24):3988-3995. R832414 (Final)
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  • Journal Article Ham WA, Ruehl CR, Kleeman MJ. Seasonal variation of airborne particle deposition efficiency in the human respiratory system. Aerosol Science and Technology 2011;45(7):795-804. R832414 (Final)
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  • Journal Article Kennedy IM. The health effects of combustion-generated aerosols. Proceedings of the Combustion Institute 2007;31(2):2757-2770. R832414 (2009)
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  • Journal Article Kleeman MJ, Riddle SG, Jakober CA. Size distribution of particle-phase molecular markers during a severe winter pollution episode. Environmental Science & Technology 2008;42(17):6469-6475. R832414 (2009)
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  • Journal Article Kleeman MJ, Riddle SG, Robert MA, Jakober CA, Fine PM, Hays MD, Schauer JJ, Hannigan MP. Source apportionment of fine (PM1.8) and ultrafine (PM0.1) airborne particulate matter during a severe winter pollution episode. Environmental Science & Technology 2009;43(2):272-279. R832414 (2010)
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  • Journal Article Lee DY, Wexler AS, Fanucchi MV, Plopper CG. Expiration rate drives human airway design. Journal of Theoretical Biology 2008;253(2):381-387. R832414 (2009)
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  • Journal Article Lee DY, Wallis C, Wexler AS, Schelegle ES, Van Winkle LS, Plopper CG, Fanucchi MV, Kumfer B, Kennedy IM, Chan JKW. Small particles disrupt postnatal airway development. Journal of Applied Physiology 2010;109(4):1115-1124. R832414 (Final)
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  • Journal Article Lee DY, Wallis CD, Van Winkle LS, Wexler AS. Disruption of tracheobronchial airway growth following postnatal exposure to ozone and ultrafine particles. Inhalation Toxicology 2011;23(9):520-531. R832414 (Final)
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  • Journal Article Lee DY, Wexler AS. Simulated annealing implementation with shorter Markov chain length to reduce computational burden and its application to the analysis of pulmonary airway architecture. Computers in Biology and Medicine 2011;41(8):707-715. R832414 (Final)
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  • Journal Article Lee DY, Willits N, Wexler AS. Detecting alterations in pulmonary airway development with airway-by-airway comparison. Annals of Biomedical Engineering 2011;39(6):1805-1814. R832414 (Final)
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  • Journal Article Lee DY, Srirama PK, Wallis C, Wexler AS. Postnatal growth of tracheobronchial airways of Sprague-Dawley rats. Journal of Anatomy 2011;218(6):717-725. R832414 (Final)
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  • Journal Article Lee DY, Wexler AS. Particle deposition in juvenile rat lungs: a model study. Journal of Aerosol Science 2011;42(9):567-579. R832414 (Final)
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  • Journal Article Lee D, Park SS, Ban-Weiss GA, Fanucchi MV, Plopper CG, Wexler AS. Bifurcation model for characterization of pulmonary architecture. Anatomical Record 2008;291(4):379-389. R832414 (2009)
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  • Journal Article Lee D, Fanucchi MV, Plopper CG, Fung J, Wexler AS. Pulmonary architecture in the conducting regions of six rats. Anatomical Record 2008;291(8):916-926. R832414 (2009)
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  • Journal Article Madl AK, Pinkerton KE. Health effects of inhaled engineered and incidental nanoparticles. Critical Reviews in Toxicology 2009;39(8):629-658. R832414 (2010)
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  • Journal Article Madl AK, Teague SV, Qu Y, Masiel D, Evans JE, Guo T, Pinkerton KE. Aerosolization system for experimental inhalation studies of carbon-based nanomaterials. Aerosol Science and Technology 2012;46(1):94-107. R832414C003 (Final)
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  • Journal Article Nakayama Wong LS, Lame MW, Jones AD, Wilson DW. Differential cellular responses to protein adducts of naphthoquinone and monocrotaline pyrrole. Chemical Research in Toxicology 2010;23(9):1504-1513. R832414 (2010)
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  • Journal Article Nakayama Wong LS, Aung HH, Lame MW, Wegesser TC, Wilson DW. Fine particulate matter from urban ambient and wildfire sources from California's San Joaquin Valley initiate differential inflammatory, oxidative stress, and xenobiotic responses in human bronchial epithelial cells. Toxicology In Vitro 2011;25(8):1895-1905. R832414 (Final)
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  • Journal Article Ngo MA, Pinkerton KE, Freeland S, Geller M, Ham W, Cliff S, Hopkins LE, Kleeman MJ, Kodavanti UP, Meharg E, Plummer L, Recendez JJ, Schenker MB, Sioutas C, Smiley-Jewell S, Haas C, Gutstein J, Wexler AS. Airborne particles in the San Joaquin Valley may affect human health. California Agriculture 2010;64(1):12-16. R832414 (2010)
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  • Journal Article Palko HA, Fung JY, Louie AY. Positron emission tomography:a novel technique for investigating the biodistribution and transport of nanoparticles. Inhalation Toxicology 2010;22(8):657-688. R832414 (Final)
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  • Journal Article Pham H, Bonham AC, Pinkerton KE, Chen CY. Central neuroplasticity and decreased heart rate variability after particulate matter exposure in mice. Environmental Health Perspectives 2009;117(9):1448-1453. R832414 (2010)
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  • Journal Article Pinkerton KE, Joad JP. Influence of air pollution on respiratory health during perinatal development. Clinical and Experimental Pharmacology and Physiology 2006;33(3):269-272. R832414 (2009)
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  • Journal Article Plummer LE, Smiley-Jewell S, Pinkerton KE. Impact of air pollution on lung inflammation and the role of Toll-like receptors. International Journal of Interferon, Cytokine and Mediator Research 2012;4:43-57. R832414 (Final)
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  • Journal Article Plummer LE, Ham W, Kleeman MJ, Wexler A, Pinkerton KE. Influence of season and location on pulmonary response to California's San Joaquin Valley airborne particulate matter. Journal of Toxicology and Environmental Health-Part A 2012;75(5):253-271. R832414 (Final)
    R832414C003 (Final)
  • Abstract from PubMed
  • Abstract: Taylor&Francis-Abstract
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  • Journal Article Riddle SG, Robert MA, Jakober CA, Hannigan MP, Kleeman MJ. Size-resolved source apportionment of airborne particle mass in a roadside environment. Environmental Science & Technology 2008;42(17):6580-6586. R832414 (2009)
    R832414 (Final)
    R832414C003 (2008)
    R832414C003 (2009)
    R832414C003 (Final)
  • Abstract from PubMed
  • Full-text: ACS-Full Text HTML
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  • Abstract: ACS-Abstract
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  • Other: ACS-Full Text PDF
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  • Journal Article Ruehl CR, Ham WA, Kleeman MJ. Temperature-induced volatility of molecular markers in ambient airborne particulate matter. Atmospheric Chemistry and Physics 2011;11(1):67-76. R832414 (2010)
    R832414C003 (2010)
  • Full-text: Atmospheric Chemistry and Physics PDF
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  • Abstract: Atmospheric Chemistry and Physics
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  • Journal Article Schenker MB, Pinkerton KE, Mitchell D, Vallyathan V, Elvine-Kreis B, Green FHY. Pneumoconiosis from agricultural dust exposure among young California farmworkers. Environmental Health Perspectives 2009;117(6):988-994. R832414 (2010)
    R832414C003 (2009)
    R832414C003 (2010)
    R832414C003 (Final)
    R826246 (Final)
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  • Abstract from PubMed
  • Associated PubMed link
  • Journal Article Sekizawa Si, Joad JP, Pinkerton KE, Bonham AC. Distinct tachykinin NK1 receptor function in primate nucleus tractus solitarius neurons is dysregulated after second-hand tobacco smoke exposure. British Journal of Pharmacology 2011;163(4):782-791. R832414 (Final)
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  • Abstract from PubMed
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  • Full-text: Wiley-Full Text HTML
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  • Abstract: Wiley-Abstract
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  • Other: Wiley-Full Text PDF
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  • Journal Article Sekizawa S-i, Joad JP, Pinkerton KE, Bonham AC. Secondhand tobacco smoke exposure differentially alters nucleus tractus solitarius neurons at two different ages in developing non-human primates. Toxicology and Applied Pharmacology 2010;242(2):199-208. R832414 (2010)
    R832414C003 (2010)
    R832414C003 (Final)
  • Abstract from PubMed
  • Full-text: Science Direct
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  • Abstract: Science Direct
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  • Other: Science Direct
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  • Journal Article Shen H, Barakat AI, Anastasio C. Generation of hydrogen peroxide from San Joaquin Valley particles in a cell-free solution. Atmospheric Chemistry and Physics 2011;11(2):753-765. R832414 (2010)
    R832414 (Final)
    R832414C002 (2010)
    R832414C002 (Final)
  • Full-text: ACP-Full Text PDF
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  • Abstract: ACP-Abstract
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  • Journal Article Shen H, Anastasio C. Formation of hydroxyl radical from San Joaquin Valley particles extracted in a cell-free surrogate lung fluid. Atmospheric Chemistry and Physics 2011;11(18):9671-9682. R832414 (Final)
    R832414C002 (Final)
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  • Abstract from PubMed
  • Associated PubMed link
  • Full-text: ACP-Full Text PDF
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  • Abstract: ACP-Abstract
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  • Journal Article Shen H, Anastasio C. A comparison of hydroxyl radical and hydrogen peroxide generation in ambient particle extracts and laboratory metal solutions. Atmospheric Environment 2012;46:665-668. R832414 (Final)
    R832414C002 (Final)
  • Full-text from PubMed
  • Abstract from PubMed
  • Associated PubMed link
  • Full-text: ScienceDirect-Full Text HTML
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  • Abstract: ScienceDirect-Abstract
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  • Other: ScienceDirect-Full Text PDF
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  • Journal Article Smith KR, Veranth JM, Kodavanti UP, Aust AE, Pinkerton KE. Acute pulmonary and systemic effects of inhaled coal fly ash in rats: comparison to ambient environmental particles. Toxicological Sciences 2006;93(2):390-399. R832414 (Final)
    R832414C003 (2006)
    R832414C003 (2007)
    R832414C003 (2008)
    R832414C003 (Final)
    R829215 (Final)
  • Abstract from PubMed
  • Full-text: OUP-Full Text HTML
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  • Abstract: Oxford-Abstract
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  • Other: OUP-Full Text PDF
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  • Journal Article Srirama PK, Wallis CD, Lee DY, Wexler AS. Imaging extra-thoracic airways and deposited particles in laboratory animals. Journal of Aerosol Science 2012;45:40-49. R832414 (Final)
    R832414C005 (Final)
  • Full-text: ScienceDirect-Full Text HTML
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  • Abstract: ScienceDirect-Abstract
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  • Other: ScienceDirect-Full Text PDF
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  • Journal Article Sutherland KM, Combs TJ, Edwards PC, Van Winkle LS. Site-specific differences in gene expression of secreted proteins in the mouse lung: comparison of methods to show differences by location. Journal of Histochemistry and Cytochemistry 2010;58(12):1107-1119. R832414 (Final)
    R832414C001 (2010)
    R832414C001 (Final)
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  • Full-text: JHC-Full Text HTML
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  • Abstract: JHC-Abstract
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  • Other: JHC-Full Text PDF
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  • Journal Article Sutherland KM, Edwards PC, Combs TJ, Van Winkle LS. Sex differences in the development of airway epithelial tolerance to naphthalene. American Journal of Physiology-Lung Cellular and Molecular Physiology 2012;302(1):L68-L81. R832414 (Final)
    R832414C001 (Final)
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  • Full-text: AJP-Full Text HTML
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  • Abstract: AJP-Abstract
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  • Other: AJP-Full Text PDF
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  • Journal Article Tablin F, den Hartigh LJ, Aung HH, Lame MW, Kleeman MJ, Ham W, Norris JW, Pombo M, Wilson DW. Seasonal influences on CAPs exposures:differential responses in platelet activation, serum cytokines and xenobiotic gene expression. Inhalation Toxicology 2012;24(8):506-517. R832414 (Final)
    R832414C002 (Final)
  • Abstract from PubMed
  • Abstract: Taylor and Francis-Abstract
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  • Journal Article Tebockhorst S, Lee D, Wexler AS, Oldham MJ. Interaction of epithelium with mesenchyme affects global features of lung architecture: a computer model of development. Journal of Applied Physiology 2007;102(1):294-305. R832414 (2009)
    R832414C005 (2007)
    R832414C005 (2008)
    R832414C005 (Final)
  • Abstract from PubMed
  • Full-text: JAP-full text HTML
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  • Abstract: JAP
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  • Other: JAP-full text PDF
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  • Journal Article Van Winkle LS, Chan JK, Anderson DS, Kumfer BM, Kennedy IM, Wexler AS, Wallis C, Abid AD, Sutherland KM, Fanucchi MV. Age specific responses to acute inhalation of diffusion flame soot particles:cellular injury and the airway antioxidant response. Inhalation Toxicology 2010;22(Suppl 2):70-83. R832414 (Final)
    R832414C001 (2010)
    R832414C001 (Final)
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  • Associated PubMed link
  • Abstract: Taylor and Francis-Abstract
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  • Journal Article Wang L, Green FHY, Smiley-Jewell SM, Pinkerton KE. Susceptibility of the aging lung to environmental injury. Seminars in Respiratory and Critical Care Medicine 2010;31(5):539-553. R832414 (Final)
    R829215 (Final)
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  • Abstract: SRCCM-Abstract
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  • Journal Article Wegesser TC, Pinkerton KE, Last JA. California wildfires of 2008: coarse and fine particulate matter toxicity. Environmental Health Perspectives 2009;117(6):893-897. R832414 (2010)
    R832414C003 (2009)
    R832414C003 (2010)
    R832414C003 (Final)
  • Full-text from PubMed
  • Abstract from PubMed
  • Associated PubMed link
  • Full-text: EHP
  • Abstract: EHP
  • Journal Article Wegesser TC, Franzi LM, Mitloehner FM, Eiguren-Fernandez A, Last JA. Lung antioxidant and cytokine responses to coarse and fine particulate matter from the great California wildfires of 2008. Inhalation Toxicology 2010;22(7):561-570. R832414 (Final)
  • Abstract from PubMed
  • Abstract: Taylor&Francis-Abstract
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  • Journal Article Wells MA, Abid A, Kennedy IM, Barakat AI. Serum proteins prevent aggregation of Fe2O3 and ZnO nanoparticles. Nanotoxicology 2012;6(8):837-846. R832414 (Final)
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  • Abstract from PubMed
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  • Full-text: ResearchGate-Full Text PDF
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  • Abstract: Taylor&Francis-Abstract
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  • Journal Article Wexler AS, Johnston MV. What have we learned from highly time-resolved measurements during EPA's Supersites Program and related studies? Journal of the Air & Waste Management Association 2008;58(2):303-319. R832414 (Final)
    R832414C005 (Final)
  • Abstract from PubMed
  • Full-text: Taylor&Francis-Full Text PDF
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  • Abstract: Taylor&Francis-Abstract
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  • Journal Article Wilson DW, Aung HH, Lame MW, Plummer L, Pinkerton KE, Ham W, Kleeman M, Norris JW, Tablin F. Exposure of mice to concentrated ambient particulate matter results in platelet and systemic cytokine activation. Inhalation Toxicology 2010;22(4):267-276. R832414 (2010)
    R832414 (Final)
    R832414C002 (Final)
    R832414C003 (2010)
    R832414C003 (Final)
  • Abstract from PubMed
  • Abstract: Taylor&Francis-Abstract
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  • Journal Article Zhong C-Y, Zhou Y-M, Smith KR, Kennedy IM, Chen C-Y, Aust AE, Pinkerton KE. Oxidative injury in the lungs of neonatal rats following short-term exposure to ultrafine iron and soot particles. Journal of Toxicology and Environmental Health, Part A-Current Issues 2010;73(12):837-847. R832414 (2010)
    R832414C003 (2010)
    R832414C003 (Final)
    R829215 (Final)
  • Abstract from PubMed
  • Full-text: ResearchGate-Full Text PDF
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  • Abstract: Taylor&Francis-Abstract
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  • Supplemental Keywords:

    ambient air, ozone, exposure, health effects, human health, metabolism, sensitive populations, infants, children, PAH, metals, oxidants, agriculture, transportation,

    Relevant Websites:

    http://saherc.ucdavis.edu Exit

    Progress and Final Reports:

    Original Abstract
  • 2007 Progress Report
  • 2008 Progress Report
  • 2009 Progress Report
  • 2010 Progress Report
  • Final Report
  • Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R832414C001 Project 1 -- Pulmonary Metabolic Response
    R832414C002 Endothelial Cell Responses to PM—In Vitro and In Vivo
    R832414C003 Project 3 -- Inhalation Exposure Assessment of San Joaquin Valley Aerosol
    R832414C004 Project 4 -- Transport and Fate Particles
    R832414C005 Project 5 -- Architecture Development and Particle Deposition