2003 Progress Report: Biological Effects of Epoxy Fatty AcidsEPA Grant Number: R829479C011
Subproject: this is subproject number 011 , established and managed by the Center Director under grant R829479
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program
Center Director: Schumacher, Dorin
Title: Biological Effects of Epoxy Fatty Acids
Investigators: Sedlacek, John D.
Institution: Kentucky State University
EPA Project Officer: Lasat, Mitch
Project Period: January 1, 2003 through December 31, 2004
Project Period Covered by this Report: January 1, 2003 through December 31, 2004
RFA: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program (2001) RFA Text | Recipients Lists
Research Category: Targeted Research , Hazardous Waste/Remediation
The objectives for the overall joint research between Kentucky State University and the University of Kentucky are to: (1) isolate epoxy fatty acids from suitable natural sources that are models for oilseed improvement such as Vernonia seeds; (2) produce other epoxy fatty acids using in vitro enzymes, microorganisms, and engineered plant tissues; (3) test the effects of these epoxy fatty acids on stored-product insect pests and model bacteria; and (4) test some of these epoxy compounds in the laboratory as a new, natural, pest-control approach for vegetable insect pests.
Dr. David Hildebrand's laboratory at the University of Kentucky will be primarily responsible for carrying out the research for Objectives 1, 2, and the later part of Objective 3, as well as some epoxy, fatty-acid biosynthesis studies (see Annual Report for STAR Grant No. R829479C003). Dr. John Sedlacek's laboratory at Kentucky State University will lead the work on storage and vegetable insect pests for Objectives 3 and 4. Thus, the results of the project reported herein represent only Objectives 3 and 4.
Preliminary bioassays have been conducted on the red flour beetle and sawtoothed grain beetle using pure castor oil, hydrolyzed castor oil, vernonia oil, vernolic acid, and vernolate. These compounds were dissolved in 6 mL of hexane and added to an appropriate measure of a cornmeal based diet to administer a 250-ppm dose. Ten grams of diet individually treated with each of the compounds, or treated with hexane only, were placed in 30 g vials. Fifteen red flour beetles or sawtoothed grain beetles were added, and each of these combinations was replicated five times. Adult mortality was quantified after 1 week. Progeny emergence was quantified for 4 weeks, and duration of development per individual was calculated. At this dose, mortality, progeny emergence, and duration of development were not impacted by any of the compounds.
The dose of these epoxy fatty acids will be increased to roughly 3,000 ppm because they occur in their native seeds at concentrations of approximately 40 percent. Enough of the compounds have recently been synthesized that bioassays again may be conducted with higher concentrations. The maize weevil and striped cucumber beetle also will be assayed. Dr. Hildebrand will synthesize several new epoxy fatty acids that we will screen as well.
Journal Articles:No journal articles submitted with this report: View all 4 publications for this subproject
Supplemental Keywords:maize weevil, red flour beetle, sawtoothed grain beetle, epoxy fatty acids as insecticides., Scientific Discipline, TREATMENT/CONTROL, Sustainable Industry/Business, Geochemistry, Technology, New/Innovative technologies, Environmental Engineering, Agricultural Engineering, agrobacterium, genetics, bioengineering, epoxy fatty acids, in vitro enzymes, engineered plant tissues, oilseed improvement, plant genes, biotechnology, remediation, engineered plant tisues, bacteriacides, biological effects
Progress and Final Reports:Original Abstract
Main Center Abstract and Reports:R829479 The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program
Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R829479C001 Plant Genes and Agrobacterium T-DNA Integration
R829479C002 Designing Promoters for Precision Targeting of Gene Expression
R829479C003 aka R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C004 Negative Sense Viral Vectors for Improved Expression of Foreign Genes in Insects and Plants
R829479C005 Development of Novel Plastics From Agricultural Oils
R829479C006 Conversion of Paper Sludge to Ethanol
R829479C007 Enhanced Production of Biodegradable Plastics in Plants
R829479C008 Engineering Design of Stable Immobilized Enzymes for the Hydrolysis and Transesterification of Triglycerides
R829479C009 Discovery and Evaluation of SNP Variation in Resistance-Gene Analogs and Other Candidate Genes in Cotton
R829479C010 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals
R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C012 High Strength Degradable Plastics From Starch and Poly(lactic acid)
R829479C013 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C014 Identification of Receptors of Bacillus Thuringiensis Toxins in Midguts of the European Corn Borer
R829479C015 Coordinated Expression of Multiple Anti-Pest Proteins
R829479C016 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C017 Molecular Improvement of an Environmentally Friendly Turfgrass
R829479C018 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals. II.
R829479C019 Transgenic Plants for Bioremediation of Atrazine and Related Herbicides
R829479C020 Root Exudate Biostimulation for Polyaromatic Hydrocarbon Phytoremediation
R829479C021 Phytoremediation of Heavy Metal Contamination by Metallohistins, a New Class of Plant Metal-Binding Proteins
R829479C022 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C023 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C024 Development of Vectors for the Stoichiometric Accumulation of Multiple Proteins in Transgenic Crops
R829479C025 Chemical Induction of Disease Resistance in Trees
R829479C026 Development of Herbicide-Tolerant Hardwoods
R829479C027 Environmentally Superior Soybean Genome Development
R829479C028 Development of Efficient Methods for the Genetic Transformation of Willow and Cottonwood for Increased Remediation of Pollutants
R829479C029 Development of Tightly Regulated Ecdysone Receptor-Based Gene Switches for Use in Agriculture
R829479C030 Engineered Plant Virus Proteins for Biotechnology