Anaerobic Biodegradation of 2,4,6-Trinitrotoluene and Other Nitroaromatic Compounds by Clostridium Acetobutylicum.EPA Grant Number: R825513C006
Subproject: this is subproject number 006 , established and managed by the Center Director under grant R825513
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: HSRC (1989) - South and Southwest HSRC
Center Director: Reible, Danny D.
Title: Anaerobic Biodegradation of 2,4,6-Trinitrotoluene and Other Nitroaromatic Compounds by Clostridium Acetobutylicum.
Investigators: Hughes, Joseph B , Bennett, G. , Rudolph, F.
Institution: Louisiana State University - Baton Rouge
EPA Project Officer: Hahn, Intaek
Project Period: January 1, 1995 through January 1, 1998
Project Amount: Refer to main center abstract for funding details.
RFA: Hazardous Substance Research Centers - HSRC (1989) RFA Text | Recipients Lists
Research Category: Hazardous Substance Research Centers , Land and Waste Management
The overarching goal of this project was to delineate the metabolism of nitroaromatics by Clostridium acetobutylicum and provide fundamental information on the biotransformation of nitroaromatic compounds under anaerobic conditions. The focus on these studies was on the metabolism of TNT. Specific tasks were : 1) to analyze the TNT biotransformation pathways of TNT by Clostridium acetobutylicum and identify intermediates and end products; 2) evaluate the effect of environmental conditions on transformation rates and end products; and 3) test the ability of Clostridium acetobutylicum to transform munitions-related nitroaromatics.
The experiments proposed were 1) to determine the pathway of biotransformation of TNT by C. acetobutylicum, 2) to study TNT biotransformation rates and evaluate the effect of environmental factors on rates, pathways, and final products, and 3) to screen the ability of C. acetobutylicum to transform other explosives related nitroaromatics.
To study TNT biotransformation rates and evaluate the effect of environmental factors on rates, pathways, and final productsexperiments were conducted in anaerobic serum bottles. They were inoculated with C. acetobutylicum under a range of temperatures (10 to 30 C), pH (5 to 8), contaminant levels (10 mg/L in aqueous systems to 1000 mg/kg in sediment systems), and sediment/water ratios (0% to 50%). These experiments also used radiolabeled TNT to facilitate mass balances, allows for fractionation of samples and tracking end product distribution.
Testing for the ability of Clostridium acetobutylicum to transform munitions-related nitroaromatics were conducted at conditions that demonstrate the highest rates of TNT transformation, followed by tests at conditions more representative of the natural environment. Experimental systems are identical to those described above.
Publications and Presentations:Publications have been submitted on this subproject: View all 22 publications for this subproject | View all 392 publications for this center
Journal Articles:Journal Articles have been submitted on this subproject: View all 7 journal articles for this subproject | View all 106 journal articles for this center
Supplemental Keywords:bioremediation, TNT, and microbial transformation., RFA, Scientific Discipline, Water, Waste, Ecosystem Protection/Environmental Exposure & Risk, Chemical Engineering, Contaminated Sediments, Environmental Chemistry, Analytical Chemistry, Fate & Transport, Hazardous Waste, Ecology and Ecosystems, Environmental Engineering, Hazardous, environmental technology, sediment treatment, hazardous waste management, hazardous waste treatment, risk assessment, fate and transport, contaminated marine sediment, soil and groundwater remediation, clostridium acetobutylicum, biodegradation, contaminated sediment, kinetics, chemical contaminants, contaminated soil, bioremediation of soils, marine sediments, remediation, chemical kinetics, hydrology, biotransformation, anaerobic biotransformation, extraction of metals, technology transfer, nitroaromatic compounds, heavy metals, bioremediation, CERCLA, aquifer fate and treatment, technical outreach
Progress and Final Reports:
Main Center Abstract and Reports:R825513 HSRC (1989) - South and Southwest HSRC
Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R825513C001 Sediment Resuspension and Contaminant Transport in an Estuary.
R825513C002 Contaminant Transport Across Cohesive Sediment Interfaces.
R825513C003 Mobilization and Fate of Inorganic Contaminant due to Resuspension of Cohesive Sediment.
R825513C004 Source Identification, Transformation, and Transport Processes of N-, O- and S- Containing Organic Chemicals in Wetland and Upland Sediments.
R825513C005 Mobility and Transport of Radium from Sediment and Waste Pits.
R825513C006 Anaerobic Biodegradation of 2,4,6-Trinitrotoluene and Other Nitroaromatic Compounds by Clostridium Acetobutylicum.
R825513C007 Investigation on the Fate and Biotransformation of Hexachlorobutadiene and Chlorobenzenes in a Sediment-Water Estuarine System
R825513C008 An Investigation of Chemical Transport from Contaminated Sediments through Porous Containment Structures
R825513C009 Evaluation of Placement and Effectiveness of Sediment Caps
R825513C010 Coupled Biological and Physicochemical Bed-Sediment Processes
R825513C011 Pollutant Fluxes to Aquatic Systems via Coupled Biological and Physicochemical Bed-Sediment Processes
R825513C012 Controls on Metals Partitioning in Contaminated Sediments
R825513C013 Phytoremediation of TNT Contaminated Soil and Groundwaters
R825513C014 Sediment-Based Remediation of Hazardous Substances at a Contaminated Military Base
R825513C015 Effect of Natural Dynamic Changes on Pollutant-Sediment Interaction
R825513C016 Desorption of Nonpolar Organic Pollutants from Historically Contaminated Sediments and Dredged Materials
R825513C017 Modeling Air Emissions of Organic Compounds from Contaminated Sediments and Dredged Materials title change in last year to "Long-term Release of Pollutants from Contaminated Sediment Dredged Material"
R825513C018 Development of an Integrated Optic Interferometer for In-Situ Monitoring of Volatile Hydrocarbons
R825513C019 Bioremediation of Contaminated Sediments and Dredged Material
R825513C020 Bioremediation of Sediments Contaminated with Polyaromatic Hydrocarbons
R825513C021 Role of Particles in Mobilizing Hazardous Chemicals in Urban Runoff
R825513C022 Particle Transport and Deposit Morphology at the Sediment/Water Interface
R825513C023 Uptake of Metal Ions from Aqueous Solutions by Sediments
R825513C024 Bioavailability of Desorption Resistant Hydrocarbons in Sediment-Water Systems.
R825513C025 Interactive Roles of Microbial and Spartina Populations in Mercury Methylation Processes in Bioremediation of Contaminated Sediments in Salt-Marsh Systems
R825513C026 Evaluation of Physical-Chemical Methods for Rapid Assessment of the Bioavailability of Moderately Polar Compounds in Sediments
R825513C027 Freshwater Bioturbators in Riverine Sediments as Enhancers of Contaminant Release
R825513C028 Characterization of Laguna Madre Contaminated Sediments.
R825513C029 The Role of Competitive Adsorption of Suspended Sediments in Determining Partitioning and Colloidal Stability.
R825513C030 Remediation of TNT-Contaminated Soil by Cyanobacterial Mat.
R825513C031 Experimental and Detailed Mathematical Modeling of Diffusion of Contaminants in Fluids
R825513C033 Application of Biotechnology in Bioremediation of Contaminated Sediments
R825513C034 Characterization of PAH's Degrading Bacteria in Coastal Sediments
R825513C035 Dynamic Aspects of Metal Speciation in the Miami River Sediments in Relation to Particle Size Distribution of Chemical Heterogeneity