Linkage of Stress Proteins and Toxic Alterations A Laboratory and Field Investigation

EPA Grant Number: R823297
Title: Linkage of Stress Proteins and Toxic Alterations A Laboratory and Field Investigation
Investigators: Hinton, David E. , Sanders, Brenda M. , Washburn, Barbara S.
Institution: University of California - Davis , California State University - Long Beach
EPA Project Officer: Hahn, Intaek
Project Period: July 1, 1995 through June 1, 1998
Project Amount: $474,656
RFA: Exploratory Research - Environmental Biology (1995) RFA Text |  Recipients Lists
Research Category: Biology/Life Sciences , Human Health , Aquatic Ecosystems


This highly integrated study will test the hypothesis that the accumulation of the major heat inducible stress proteins, stress-70 and chaperonin (cpn60), reflect contaminant-induced damage to tissues and subcellular compartments. Two polyaromatic hydrocarbons, benzo(a)pyrene and beta-naphthoflavone, and copper will be investigated for their effect on individuals, cellular and subcellular levels of biological organization using molecular biological, physiological and comparative pathobiological approaches. These hallmark features of response will be linked to induction and accumulation of stress proteins in rainbow trout (Oncorhynchus mykiss) and medaka (Oryzias latipes). After testing the relationship in laboratory studies under conditions of acute, acute with recovery, and chronic exposures to various life stages, we shall apply the most relevant methods to evaluate a copper contaminated field site and an upstream reference site inhabited by rainbow trout. The latter will allow us to account for potential confounding results in field versus laboratory situations. Results will identify target tissues of toxicity and will be used to better interpret meaning of stress protein responses.

Publications and Presentations:

Publications have been submitted on this project: View all 1 publications for this project

Journal Articles:

Journal Articles have been submitted on this project: View all 1 journal articles for this project

Supplemental Keywords:

RFA, Toxics, Ecosystem Protection/Environmental Exposure & Risk, Ecosystem/Assessment/Indicators, Ecosystem Protection, exploratory research environmental biology, Chemical Mixtures - Environmental Exposure & Risk, HAPS, Ecological Effects - Environmental Exposure & Risk, Ecological Effects - Human Health, Ecological Indicators, fate, marine ecosystem, heat stress, stressors, hydrocarbon, hydrological, PAH, pathobiological, field validation, hydrocarbons, toxic chemicals, toxic environmental contaminants, fish , Benzene (including benzene from gasoline), exposure assessment, rainbow trout

Progress and Final Reports:

  • 1996
  • 1997 Progress Report
  • Final