Final Report: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil

EPA Grant Number: R828772
Center: HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils
Center Director: Semprini, Lewis
Title: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil
Investigators: Semprini, Lewis , Reinhard, Martin , Williamson, Kenneth J.
Institution: Oregon State University
EPA Project Officer: Lasat, Mitch
Project Period: September 1, 2001 through August 31, 2006 (Extended to August 31, 2007)
Project Amount: $5,572,000
RFA: Hazardous Substance Research Centers - HSRC (2001) RFA Text |  Recipients Lists
Research Category: Hazardous Waste/Remediation , Land and Waste Management

Objective:

The Western Region Hazardous Substance Research Center (WRHSRC) was a cooperative activity between Oregon State University and Stanford University that was renewed in October 2001. The Center was a continuation of the original Center established in 1989 to address critical hazardous substance problems in EPA Regions 9 and 10. The regions include the states of Alaska, Arizona, California, Hawaii, Idaho, Nevada, Oregon, and Washington, and Guam. The research focus of the Center was to develop in situ processes for VOC remediation in groundwater and Soil.

Goals:
The goals of the Center were to:

  1. Develop innovative technologies for the in situ treatment of volatile organic chemicals (VOCs) in groundwater, especially chlorinated solvents.
  2. Increase the number, speed, and efficiency of available treatment options for both high concentration source zones and diffuse contamination plumes.
  3. Disseminate the results of research to the industrial and regulatory communities, to foster exchange of information with these communities, and to promote a better understanding of the scientific capability to detect, assess, and mitigate risks associated with hazardous substance usage and disposal.

Groundwater cleanup and site remediation, with a strong emphasis on treatments that used microbes or chemical catalysts to transform VOCs into harmless substances, represented the major focus of Center’s research activities. Research projects included biological (biotic) and physical and chemical (abiotic) treatment processes, as well as in situ characterization methods for monitoring the progress of both intrinsic and enhanced remediation. In combination with basic laboratory and field studies, physical and mathematical models were developed to study these processes and to provide a bridge between theory and practice. The technology transfer program involved the process of taking new technologies from the laboratory to the field. Center researchers worked with other federal agencies, such as the Department of Defense (DoD) and private industry, in conducting field evaluations of new technologies. Technical Outreach Services for Communities (TOSC) was a technical assistance program designed to aid communities confronted with environmental contamination by hazardous waste sites. TOSC provided interested community groups with technical information and assistance that enabled early and meaningful public participation in decisions that affect health and welfare. The Center’s Technical Assistance to Brownfields Communities (TAB) program provided assistance to communities attempting to address cleanup and redevelopment of properties whose reuse has been prevented by real or perceived contamination. TAB attempted to improve involvement of all affected parties in cleanup and redevelopment process through education and training.

Lewis Semprini was the director of the Center and of the research program. Kenneth J. Williamson served as associate director in charge of training, technology transfer and community outreach. Martin Reinhard, the assistant director, was in charge of the Center's quality control program. Garrett Jones was the Center's administrative assistant. Fifteen OSU and Stanford faculty members were involved in the Center. They collectively represented an integrated research group of many different disciplines, including biochemistry, chemistry, environmental engineering, environmental chemistry, geosciences, hydrogeology, molecular biology, microbiology, public health, and sociology.

The Center had two major advisory groups to guide its activities. The Science Advisory Committee (SAC) had oversight for all Center research activities and technology transfer activities, and the Outreach Advisory Committee (OAC) oversaw the Center's TOSC and TAB programs. The members of the SAC and OAC represented federal and state governments, industry, consulting firms, and universities. Experts with a broad range of expertise were included in the SAC and the OAC.

The Center received a total of $3,763,200 of funds from the EPA. The EPA required matching funds at a level of 20%. Oregon State University allocated $405,000 in funds to help meet this required match.

Summary/Accomplishments (Outputs/Outcomes):

The final report for the Western Region Hazardous Substance Research Center provides an overview of the accomplishments of the Center for the period of October 2001 to August 2007. The Center funded twelve research projects and an Outreach Center for the TOSC and TAB programs. The report provides final summary reports for all the Center’s projects. Publications that resulted from the Center’s research projects are also listed as well as publications that resulted from the field demonstrations. Provided below are the research projects and the PIs and the Co-PIs of the projects.

Research Projects

1-SU-01 Strategies for Cost-Effective In Situ Mixing of Contaminants and Additives in Bioremediation. Peter K. Kitanidis, PI; Craig S. Criddle, Co-PI, Stanford University

1-OSU-01 Developing and Optimizing Biotransformation Kinetics for the Bioremediation of Trichloroethylene at NAPL Source Zone Concentrations. Lewis Semprini, PI; Mark E. Dolan, Co-PI, Oregon State University

1-OSU-02 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms. Peter Bottomley, PI; Daniel J. Arp Lynda Ciuffetti, Stephen Giovannoni, Lewis Semprini, Ken Williamson, Mark Dolan, Co-PIs, Oregon State University.

1-SU-02 Chemical, Physical and Biological Processes at the Surface of Palladium Catalysts under Groundwater Treatment Conditions. Martin Reinhard, PI and John Westall, Co-PI, Stanford and Oregon State Universities.

1-SU-03 Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers. Martin Reinhard, PI, Stanford University

1-OSU-03 Development of the Push-Pull Test to Monitor Bioaugmentation with Dehalogenating Cultures Jennifer A. Field, PI; Jonathan D. Istok, M.E. Dolan Co-PI, Oregon State University

1-OSU-04 Development and Evaluation of Field Sensors for Monitoring Bioaugmentation with Anaerobic Dehalogenating Cultures for In Situ Treatment of TCE. James D. Ingle, PI, Oregon State University.

2-OSU-05 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols. Peter Bottomley, PI; Daniel Arp, Mark Dolan, Lewis Semprini, Co-PIs, Oregon State University

2-OSU-06 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation. James Ingle, PI, Oregon State University

2-OSU-07 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity. Lewis Semprini, PI, Oregon State University, Mark Dolan, Co-PI, Oregon State University, Alfred Spormann, Co-PI, Stanford University

2-SU-04 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media Peter K. Kitanidis, PI; Craig Criddle, Stanford Co-PI, Stanford University

2-SU-05 Sorption and Hydrolysis of Halogenated Hydrocarbons in Soil Nanopores. Martin Reinhard, PI, Stanford University

Doctoral and Master’s Degrees
One of the most import functions of the Center was the education of the next generation of researchers, educators, and practitioners in the area of subsurface remediation. A total of 19 Ph.D. students, five M.S. students, and one post-doctoral student were supported through the Center’s funding research projects.

Technology Transfer
Center researchers were involved with technology transfer through involvement in field demonstration projects, supporting the development of Research Briefs, and the publication of the results of their research, as previously described. Six field demonstration projects that resulted from basis or applied research funded by the Center. These field demonstration projects were funded by other governmental agencies such as Environmental Security Technology Certification Program (ESTCP) and the Strategic Environmental Research and Development Program (SERDP) of the Department of Defense. Several field demonstrations were also supported by Chevron-Texaco Corporation and Textron Corporation. Two of the projects, one funded by ESTCP and another by SERDP, were associated with demonstrations and the development of protocols to assess aerobic cometabolism. Another ESTCP project was centered on using radon-222 as in situ tracer for monitoring the remediation of NAPL contamination. A third ESTCP project evaluated Pd-catalysts for the in situ treatment of TCE using horizontal wells. Chevron-Texaco and Textron Corporation supported field push-pull test demonstrations using trichlorofluoroethene as a reactive tracer for evaluating TCE remediation.

To aid in technology transfer, a Website was developed and maintained during the life of the Center. The web site http://wrhsrc.oregonstate.edu/ Exit provided an overview of the WRHSRC and links to publications and project information. The website included a description of the HSRC program and WRHSRC goals and management; links and contact information for center research and outreach staff; descriptions of research focus areas and projects; a searchable database of WRHSRC publications and previous projects (http://wrhsrc.oregonstate.edu/publications/index.htm Exit ); descriptions of center outreach programs and links to the separate Websites for the Western Region TOSC/TAB programs; a news and events page with regular postings; and an opportunity to sign up to receive electronic newsletters from the WRHSRC and the TOSC/TAB programs. The Web site received an average of 1400 visits per month. Common search terms that lead visitors to the site include reductive dechlorination, cometabolism, anaerobic processes, palladium catalysts, and individual researchers’ names. The Research Briefs were among the most commonly viewed pages.

Another product of the technology transfer program was Research Briefs, which provided short summaries of Center projects and also emphasized research applications and demonstration projects. The Research Briefs were advertised through the Center electronic newsletter and announced on other electronic mailing lists read by the groundwater remediation community such as Tech Direct, USEPA Region 10 Science Forum (intranet site), and USEPA Region 9 Hazardous Substance Technical Liaison Newsletter. Over the life of the Center, ten Research Briefs were written. These Research Briefs at located at the following Web address: (http://wrhsrc.oregonstate.edu/briefs/index.htm Exit ).

TOSC and TAB
The Technical Outreach Services for Communities (TOSC) Program was a technical assistance project designed to aid communities confronted with environmental contamination by hazardous waste sites. The TOSC and TAB were funded directly through the Center, or through collateral grants. TOSC provided interested community groups with technical information and assistance to encourage early and meaningful public participation in decisions that affect their health and welfare. The TOSC program provided a viable alternative source of assistance for communities that did not qualify for a Technical Assistance Grant (TAG) from the US Environmental Protection Agency. Centered at Oregon State University, the TOSC team involved university faculty and students, as well as contracted environmental professionals with specialization in environmental engineering, risk communication, public health, information transfer, environmental justice, and community relations. Where appropriate, WR TOSC partnered with staff of the Technical Outreach Services for Native American Communities (TOSNAC) to provide assistance to tribal communities. From 2001 to 2007 TOSC provided technical assistance to 36 communities in Region 9 and 10. The TAB provided assistance to 17 Brownfields projects. The program also helped organize two Oregon Rural Brownfields Conferences (2001, 2002) and two Oregon Brownfields Conferences (2005, 2007).

A complete list of publications is provided on the Center’s Website: http://wrhsrc.oregonstate.edu/publications/index.htm Exit


Journal Articles: 60 Displayed | Download in RIS Format

Other center views: All 158 publications 63 publications in selected types All 60 journal articles
Type Citation Sub Project Document Sources
Journal Article Achong GR, Rodriguez AM, Spormann AM. Benzylsuccinate synthase of Azoarcus sp. strain T: cloning, sequencing, transcriptional organization, and its role in anaerobic toluene and m-xylene mineralization. Journal of Bacteriology 2001;183(23):6763-6770. R828772 (Final)
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  • Journal Article Arp DJ, Yeager CM, Hyman MR. Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene. Biodegradation 2001;12(2):81-103. R828772 (2002)
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  • Journal Article Benekos ID, Cirpka OA, Kitanidis PK. Experimental determination of transverse dispersivity in a helix and a cochlea. Water Resources Research 2006;42:W07406, doi:10.1029/2005WR004712. R828772 (Final)
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  • Journal Article Cantrell KM, Ingle Jr JD. The SLIM spectrometer. Analytical Chemistry 2003;75(1):27-35. R828772 (2002)
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  • Journal Article Cantrell K, Ingle Jr. JD. Design and evaluation of a membrane sampling spectrometer array for real-time, in-situ depth profiling of sub-surface waters. Aquatic Sciences-Research Across Boundaries. R828772 (2003)
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    Journal Article Cheng H, Reinhard M. Sorption of trichloroethylene in hydrophobic micropores of dealuminated Y zeolites and natural minerals. Environmental Science & Technology 2006;40(24):7694-7701. R828772 (Final)
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  • Journal Article Cheng H, Reinhard M. Measuring hydrophobic micropore volumes in geosorbents from trichloroethylene desorption data. Environmental Science & Technology 2006;40(11):3595-3602. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cheng H, Reinhard M. Sorption and inhibited dehydrohalogenation of 2,2-dichloropropane in micropores of dealuminated Y zeolites. Environmental Science & Technology 2007;41(6):1934-1941. R828772 (Final)
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  • Journal Article Chu M, Kitanidis PK, McCarty PL. Effects of biomass accumulation on microbially enhanced dissolution of a PCE pool: a numerical simulation. Journal of Contaminant Hydrology 2003;65(1-2):79-100. R828772 (2002)
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  • Journal Article Cunningham JA, Deitsch JJ, Smith JA, Reinhard M. Quantification of contaminant sorption-desorption time-scales from batch experiments. Environmental Toxicology and Chemistry 2005;24(9):2160-2166. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cupples AM, Spormann AM, McCarty PL. Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR. Applied and Environmental Microbiology 2003;69(2):953-959. R828772 (2002)
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  • Journal Article Cupples AM, Spormann AM, McCarty PL. Comparative evaluation of chloroethene dechlorination to ethene by Dehalococcoides-like microorganisms. Environmental Science & Technology 2004;38(18):4768-4774. R828772 (2004)
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  • Journal Article Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Effects of dichloroethene isomers on the induction and activity of butane monooxygenase in the alkane-oxidizing bacterium “Pseudomonas butanovora.” Applied and Environmental Microbiology 2005;71(10):6054-6059. R828772 (2003)
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  • Journal Article Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Product repression of alkane monooxygenase expression in Pseudomonas butanovora. Journal of Bacteriology 2006;188(7):2586-2592. R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Doughty DM, Halsey KH, Vieville CJ, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Propionate inactivation of butane monooxygenase activity in ‘Pseudomonas butanovora’: biochemical and physiological implications. Microbiology 2007;153(Pt 11):3722-3729. R828772 (Final)
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  • Journal Article Doughty DM, Kurth EG, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Evidence for involvement of copper ions and redox state in regulation of butane monooxygenase in Pseudomonas butanovora. Journal of Bacteriology 2008;190(8):2933-2938. R828772 (Final)
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  • Journal Article Doughty DM, Arp DJ, Bottomley PJ. Dichloroethylenes as substrates and inducers of butane monooxygenase in Pseudomonas butanovora. Microbiology. R828772 (2003)
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    Journal Article Dupin HJ, Kitanidis PK, McCarty PL. Pore-scale modeling of biological clogging due to aggregate expansion: a material mechanics approach. Water Resources Research 2001;37(12):2965-2979. R828772 (2002)
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  • Journal Article Dupin HJ, Kitanidis PK, McCarty PL. Simulations of two-dimensional modeling of biomass aggregate growth in network models. Water Resources Research 2001;37(12):2981-2994. R828772 (2002)
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  • Journal Article Field JA, Reed RL, Istok JD, Semprini L, Bennett P, Buscheck TE. Trichlorofluoroethene: a reactive tracer for evaluating reductive dechlorination in large-diameter permeable columns. Ground Water Monitoring & Remediation 2005;25(2):68-77. R828772 (2005)
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  • Journal Article Fienen MN, Luo J, Kitanidis PK. Semi-analytical homogeneous anisotropic capture zone delineation. Journal of Hydrology 2005;312(1-4):39-50. R828772 (2003)
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  • Journal Article Fienen MN, Luo J, Kitanidis PK. A Bayesian geostatistical transfer function approach to tracer test analysis. Water Resources Research 2006;42:W07426, doi:10.1029/2005WR004576. R828772 (Final)
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  • Journal Article Frascari D, Kim Y, Dolan ME, Semprini L. A kinetic study of aerobic propane uptake and cometabolic degradation of chloroform, cis-dichloroethylene and trichloroethylene in microcosms with groundwater/aquifer solids. Water, Air, & Soil Pollution: Focus 2003;3(3):285-298. R828772 (2002)
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  • Journal Article Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater. 1. Dynamics of a recirculating well system. Water Resources Research 2002;38(4):1039, doi:10.1029/2001WR000379. R828772 (2002)
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  • Journal Article Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater. 2. Comprehensive analysis of field data using reactive transport modeling. Water Resources Research 2002;38(4):1040, doi:10.1029/2001WR000380. R828772 (2002)
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  • Journal Article Goltz MN, Williamson KJ. Transfer and commercialisation of contaminated groundwater remediation technologies. International Journal of Technology Transfer and Commercialisation 2002;1(4):329-346. R828772 (2002)
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  • Journal Article Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Trichloroethylene degradation by butane-oxidizing bacteria causes a spectrum of toxic effects. Applied Microbiology and Biotechnology 2005;68(6):794-801. R828772 (2003)
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  • Journal Article Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Site-directed amino acid substitutions in the hydroxylase α subunit of butane monooxygenase from Pseudomonas butanovora: implications for substrates knocking at the gate. Journal of Bacteriology 2006;188(13):4962-4969. R828772 (Final)
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  • Journal Article Halsey KH, Doughty DM, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Evidence for modified mechanisms of chloroethene oxidation in Pseudomonas butanovora mutants containing single amino acid substitutions in the hydroxylase α-subunit of butane monooxygenase. Journal of Bacteriology 2007;189(14):5068-5074. R828772 (Final)
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  • Journal Article Johnson HA, Pelletier DA, Spormann AM. Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme. Journal of Bacteriology 2001;183(15):4536-4542. R828772 (2002)
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  • Journal Article Jones BD, Ingle Jr JD. Evaluation of immobilized redox indicators as reversible, in situ redox sensors for determining Fe(III)-reducing conditions in environmental samples. Talanta 2001;55(4):699-714. R828772 (2002)
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  • Journal Article Jones BD, Ingle Jr JD. Evaluation of redox indicators for determining sulfate-reducing and dechlorinating conditions. Water Research 2005;39(18):4343-4354. R828772 (Final)
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  • Journal Article Kim Y, Arp DJ, Semprini L. Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;80(5):498-508. R828772 (2002)
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  • Journal Article Kim Y, Arp DJ, Semprini L. A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1-trichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;77(5):564-576. R828772 (2002)
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  • Journal Article Kim Y, Semprini L. Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane-grown mixed culture. Water Science & Technology 2005;52(8):125-131. R828772 (Final)
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  • Journal Article Lee IS, Bae JH, Yang Y, McCarty PL. Simulated and experimental evaluation of factors affecting the rate and extent of reductive dehalogenation of chloroethenes with glucose. Journal of Contaminant Hydrology 2004;74(1-4):313-331. R828772 (2004)
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  • Journal Article Luo J, Kitanidis PK. Fluid residence times within a recirculation zone created by an extraction-injection well pair. Journal of Hydrology 2004;295(1-4):149-162. R828772 (2003)
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  • Journal Article Luo J, Wu W, Fienen MN, Jardine PM, Mehlhorn TL, Watson DB, Cirpka OA, Criddle CS, Kitanidis PK. A nested-cell approach for in situ remediation. Ground Water 2006;44(2):266-274. R828772 (Final)
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  • Journal Article Luo J, Cirpka OA, Kitanidis PK. Temporal-moment matching for truncated breakthrough curves for step or step-pulse injection. Advances in Water Resources 2006;29(9):1306-1313. R828772 (Final)
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  • Journal Article McMurdie PJ, Behrens SF, Holmes S, Spormann AM. Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species. Applied and Environmental Microbiology 2007;73(8):2744-2747. R828772 (Final)
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  • Journal Article Muller JA, Rosner BM, von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM. Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Applied and Environmental Microbiology 2004;70(8):4880-4888. R828772 (2004)
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  • Journal Article Munakata N, Reinhard M. Palladium-catalyzed aqueous hydrodehalogenation in column reactors:modeling of deactivation kinetics with sulfide and comparison of regenerants. Applied Catalysis B:Environmental 2007;75(1-2):1-10. R828772 (Final)
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  • Journal Article Niemet MR, Semprini L. Column studies of anaerobic carbon tetrachloride biotransformation with Hanford aquifer material. Ground Water Monitoring & Remediation 2005;25(3):82-92. R828772 (2005)
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  • Journal Article Pon G, Hyman MR, Semprini L. Acetylene inhibition of trichloroethene and vinyl chloride reductive dechlorination. Environmental Science & Technology 2003;37(14):3181-3188. R828772 (2003)
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  • Journal Article Pon G, Semprini L. Anaerobic reductive dechlorination of 1-chloro-1-fluoroethene to track the transformation of vinyl chloride. Environmental Science & Technology 2004;38(24):6803-6808. R828772 (2003)
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  • Other: ACS Publications PDF
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  • Journal Article Ruiz-Haas P, Ingle Jr JD. Monitoring redox conditions with flow-based and fiber optic sensors based on redox indicators: application to reductive dehalogenation in a bioaugmented soil column. Geomicrobiology Journal 2007;24(3-4):365-378. R828772 (Final)
  • Abstract: Informa World Abstract
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  • Journal Article Sayavedra-Soto LA, Doughty DM, Kurth EG, Bottomley PJ, Arp DJ. Product and product-independent induction of butane oxidation in Pseudomonas butanovora. FEMS Microbiology Letters 2005;250(1):111-116. R828772 (Final)
  • Abstract from PubMed
  • Full-text: Wiley HTML
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  • Abstract: Wiley
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  • Journal Article Schroth MH, Istok JD. Approximate solution for solute transport during spherical-flow push-pull tests. Ground Water 2005;43(2):280-284. R828772 (2005)
    R828772 (Final)
  • Abstract from PubMed
  • Full-text: Oregon State University PDF
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  • Abstract: Wiley
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  • Journal Article Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Laboratory, field, and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. Advances in Water Resources 2007;30(6-7):1528-1546. R828772 (Final)
  • Full-text: Science Direct HTML
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  • Abstract: Science Direct
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  • Journal Article Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. European Journal of Soil Biology 2007;43(5-6):322-327. R828772 (Final)
  • Abstract: Science Direct Abstract
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  • Journal Article Skinner KM, Martinez-Prado A, Hyman MR, Williamson KJ, Ciuffetti LM. Pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, Graphium sp. Applied Microbiology and Biotechnology 2008;77(6):1359-1365. R828772 (Final)
  • Abstract from PubMed
  • Abstract: SpringerLink
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  • Journal Article Sriwatanapongse W, Reinhard M, Klug CA. Reductive hydrodechlorination of trichloroethylene by palladium-on-alumina catalyst:13C solid-state NMR study of surface reaction precursors. Langmuir 2006;22(9):4158-4164. R828772 (Final)
  • Abstract from PubMed
  • Abstract: ACS Publications
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  • Journal Article Taylor AE, Dolan ME, Bottomley PJ, Semprini L. Utilization of fluoroethene as a surrogate for aerobic vinyl chloride transformation. Environmental Science & Technology 2007;41(18):6378-6383. R828772 (Final)
  • Abstract from PubMed
  • Full-text: ACS Publications Full Text
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  • Other: ACS Publications PDF
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  • Journal Article Yang Y, McCarty PL. Comparison between donor substrates for biologically enhanced tetrachloroethene DNAPL dissolution. Environmental Science & Technology 2002;36(15):3400-3404. R828772 (2002)
    R828772 (Final)
  • Abstract from PubMed
  • Full-text: ACS Publications Full Text
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  • Abstract: ACS Publications
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  • Journal Article Yeager CM, Bottomley PJ, Arp DJ. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Applied and Environmental Microbiology 2001;67(12):5384-5391. R828772 (2002)
    R828772 (Final)
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  • Full-text: AEM HTML
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  • Abstract: AEM
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  • Journal Article Yeager CM, Bottomley PJ, Arp DJ. Cytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4. Applied and Environmental Microbiology 2001;67(5):2107-2115. R828772 (2002)
    R828772 (Final)
    R825689C027 (Final)
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  • Abstract from PubMed
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  • Abstract: AEM-Abstract
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  • Journal Article Yeager CM, Arthur KM, Bottomley PJ, Arp DJ. Trichloroethylene degradation by toluene-oxidizing bacteria grown on non-aromatic substrates. Biodegradation 2004;15(1):19-28. R828772 (2003)
    R828772 (2004)
    R828772 (Final)
    R828772C010 (2005)
  • Abstract from PubMed
  • Abstract: SpringerLink
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  • Journal Article Yu S, Semprini L. Comparison of trichloroethylene reductive dehalogenation by microbial communities stimulated on silicon-based organic compounds as slow-release anaerobic substrates. Water Research 2002;36(20):4985-4996. R828772 (2002)
    R828772 (Final)
    R828772C001 (2001)
  • Abstract from PubMed
  • Full-text: Science Direct Full Text
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  • Abstract: Science Direct
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  • Journal Article Yu S, Semprini L. Kinetics and modeling of reductive dechlorination at high PCE and TCE concentrations. Biotechnology and Bioengineering 2004;88(4):451-464. R828772 (2004)
    R828772 (Final)
  • Abstract from PubMed
  • Full-text: Oregon State PDF
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  • Abstract: ACS Publications
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  • Journal Article Yu S, Dolan ME, Semprini L. Kinetics and inhibition of reductive dechlorination of chlorinated ethylenes by two different mixed cultures. Environmental Science & Technology 2005;39(1):195-205. R828772 (2003)
    R828772 (Final)
    R828772C012 (2005)
  • Abstract from PubMed
  • Abstract: ACS Publications
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  • Other: Oregon State University PDF
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  • Supplemental Keywords:

    groundwater, soil, in-situ, remediation, VOCs characterization, brownfields, remediation, hazardous waste, environmental chemistry, drinking water contaminants, brownfield site, biotransformation, remediation technologies, chlorinated solvent, contaminated soil, bioremediation of soil, bioremediation, technology transfer, VOCs, contaminated sites, Region 9, Region 10,, RFA, Scientific Discipline, Air, Waste, Water, POLLUTANTS/TOXICS, Contaminated Sediments, air toxics, Environmental Chemistry, Chemicals, Hazardous Waste, Brownfields, Ecology and Ecosystems, Hazardous, Groundwater remediation, Environmental Engineering, brownfield sites, environmental hazards, sediment treatment, in situ remediation, advanced treatment technologies, NAPL, chemical wastes, air pollutants, contaminant dynamics, in situ treatment, VOCs, contaminated sediment, ambient air, palladium catalysis, contaminated soil, emissions, chemical contaminants, in-situ treatment of chlorinated solvents, treatment, remediation, atmospheric aerosols, chlorinated VOCs, contaminated groundwater, air emissions, technology transfer, VOC remediation, contaminated aquifers, NAPLs, Volatile Organic Compounds (VOCs), bioremediation, chlorinated solvents, groundwater, TCE

    Relevant Websites:

    Western Region Hazardous Substance Research Center http://wrhsrc.oregonstate.edu/ Exit
    TOSC and TAB Programs of the Western Region Hazardous Substance Research Center http://tosc.oregonstate.edu/ Exit
    ComCZAT --- Complex Capture Zone Analysis Routine http://www.talulat.com/mike/software/ Exit
    A Bayesian Geostatistical Transfer Function Approach to Tracer Test Analysis - Implementation and Examples http://www.talulat.com/mike/software/lstran.html Exit

    Progress and Final Reports:

    Original Abstract
  • 2002 Progress Report
  • 2003 Progress Report
  • 2004 Progress Report
  • 2005 Progress Report
  • 2006
  • Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bio- remediation of Trichloroethylene at NAPL Source Zone Concentrations
    R828772C002 Strategies for Cost-Effective In-situ Mixing of Contaminants and Additives in Bioremediation
    R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
    R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions
    R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers
    R828772C006 Development of the Push-Pull Test to Monitor Bioaugmentation with Dehalogenating Cultures
    R828772C007 Development and Evaluation of Field Sensors for Monitoring Bioaugmentation with Anaerobic Dehalogenating Cultures for In-Situ Treatment of TCE
    R828772C008 Training and Technology Transfer
    R828772C009 Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB) Programs
    R828772C010 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
    R828772C011 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation for In Situ Treatment of PCE and TCE
    R828772C012 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity
    R828772C013 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media
    R828772C014 The Role of Micropore Structure in Contaminant Sorption and Desorption