2005 Progress Report: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil

EPA Grant Number: R828772
Center: HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils
Center Director: Semprini, Lewis
Title: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil
Investigators: Semprini, Lewis
Current Investigators: Semprini, Lewis , Reinhard, Martin , Williamson, Kenneth J.
Institution: Oregon State University
EPA Project Officer: Lasat, Mitch
Project Period: September 1, 2001 through August 31, 2006 (Extended to August 31, 2007)
Project Period Covered by this Report: September 1, 2004 through August 31, 2005
Project Amount: $5,572,000
RFA: Hazardous Substance Research Centers - HSRC (2001) RFA Text |  Recipients Lists
Research Category: Hazardous Waste/Remediation , Land and Waste Management

Objective:

The Western Region Hazardous Substance Research Center (WRHSRC) is a cooperative activity between Oregon State University (OSU) and Stanford University (SU) that was established in October 2001. The Center is a continuation of the original Center established in 1989 to address critical hazardous substance problems in U.S. Environmental Protection Agency (EPA) Regions 9 and 10. The regions include the states of Alaska, Arizona, California, Hawaii, Idaho, Nevada, Oregon, and Washington, and Guam. The Center receives its base financial support from EPA.

The objectives of the Center are:

  1. To develop innovative technologies for the in-situ treatment of volatile organic chemicals (VOCs) in groundwater, especially chlorinated solvents.
  2. To increase the number, speed, and efficiency of available treatment options for both high concentration source zones and diffuse contamination plumes.
  3. To disseminate the results of research to the industrial and regulatory communities, to foster exchange of information with these communities, and to promote a better understanding of the scientific capability to detect, assess, and mitigate risks associated with hazardous substance usage and disposal.

Groundwater cleanup and site remediation, with a strong emphasis on treatments that use microbes or chemical catalysts to transform VOCs into harmless substances, represent the major focus of Center activities. Research projects include biological (biotic) and physical and chemical (abiotic) treatment processes, as well as in-situ characterization methods for monitoring the progress of both intrinsic and the enhanced remediation. In combination with basic laboratory and field studies, physical and mathematical models are being used to study these processes and to provide a bridge between theory and practice. The technology transfer program involves the process of taking new technologies from the laboratory to the field. Center researchers are working with other federal agencies, such as the Department of Defense (DoD) and the Department of Energy (DoE), and private industry, in conducting field evaluations of new technologies. Technical Outreach Services for Communities (TOSC) is a technical assistance program designed to aid communities confronted with environmental contamination by hazardous waste sites. TOSC provides interested community groups with technical information and assistance that can enable early and meaningful public participation in decisions that affect health and welfare. The Center’s Technical Assistance to Brownfields Communities (TAB) Program provides assistance to communities attempting to address cleanup and redevelopment of properties whose reuse has been prevented by real or perceived contamination. TAB attempts to improve involvement of all affected parties in the cleanup and redevelopment process through education and training.

Fifteen OSU and Stanford faculty members are currently involved in the Center. They collectively represent an integrated research group of many different disciplines, including biochemistry, chemistry, environmental engineering, environmental chemistry, geosciences, hydrogeology, molecular biology, microbiology, public health, and sociology. Lewis Semprini is director of the Center and of the research program. Kenneth J. Williamson serves as associate director in charge of training, technology transfer and community outreach. Martin Reinhard, the assistant director, is in charge of the Center’s quality control program. Garrett Jones is the Center’s administrative assistant.

The Center has two major advisory groups to guide its activities. The Science Advisory Committee (SAC) has oversight for all Center research activities and technology transfer activities, and the Outreach Advisory Committee (OAC) oversees the Center’s TOSC and TAB programs. The members of the SAC and OAC represent federal and state governments, industry, consulting firms, and universities. Experts with a broad range of expertise are included in the SAC and the OAC.

The education of students interested in careers directed toward finding solutions to environmental problems is another important goal. Twelve graduate students have been supported during the fourth year of the Center, with ten of these being Ph.D. students. Over 60% of the Center core funds are being directed toward the graduate training of students through the Center’s research and outreach projects.

Progress Summary:

The major focus of research activities for the OSU-Stanford University WRHSRC, and indeed its major mission, has been the conduct of basic research related to the in-situ treatment of VOC subsurface contamination. During the past year, research was continued on five center projects associated with the in-situ remediation of chlorinated solvents. The projects and the researchers are summarized below in Table 1.

Table 1 . Research Project Summary

EPA
Agreement
Number

Center
Project
Number


Title

Principal Investigator (PI),
Co-PI

R828772C010

2-OSU-05

Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols

Peter Bottomley, PI, Daniel Arp, Mark Dolan, Lewis Semprini, Co-PIs, Oregon State University

R828772C011

2-OSU-06

Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation After Bioaugmentation

James Ingle, PI, Oregon State University

R828772C012

2-OSU-07

Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity

Lewis Semprini, PI, Oregon State University, Mark Dolan, Co-PI, Oregon State University, Alfred Spormann, Co-PI, Stanford University

R828772C013

2-SU-04

Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media

Peter K. Kitanidis, PI. Craig Criddle, Stanford Co-PI, Stanford University

R828772C014

2-SU-05

The Role of Micropore Structure in Contaminant Sorption and Desorption

Martin Reinhard, PI, Stanford University

Research projects include both aerobic and treatment processes (physical and reactive transport processes), as well as in-situ characterization methods for monitoring the progress of both intrinsic and the enhanced remediation. Three project PIs are at OSU and three are at Stanford University. Project 2-OSU-07 represents a joint project between Stanford University and Oregon State University on the anaerobic transformation of chlorinated solvents.

Project 2-OSU-05 (Grant No. R828772C010), which is being conducted at Oregon State University, is a collaborative project among microbiologists and engineers headed by Peter Bottomley and Mark Dolan. The goal of the project is to study the aerobic cometabolism of chlorinated ethenes by microorganisms that are grown on hydrocarbons as well as organics acids, and also to study the direct aerobic metabolism of vinyl chloride (VC) and cis-dichloroethene (cis-DCE). In a study being directed by Peter Bottomley and Daniel Arp, conditions are being identified that maximize reductant flow to cometabolism and that promote maximum expression of monooxygenase genes and enzyme activity. Mutant strains of Pseudomonas butanovora containing single amino acid substitutions to BMO were engineered. One mutant strain, in particular, degraded a larger quantity of TCE, compared to the wild type strain, albeit at a slower rate. Currently, it is being determined if mutant strains with slower turnover rates will result in sustainable TCE degradation. Preliminary in vivo studies with P. butanovora indicate both propionate and butyrate irreversibly inactivated the sBMO enzyme. The possibility that organic acids act as mechanism based inactivators of the sBMO enzyme in currently being explored with a range of organic acids.

In a study being directed by Mark Dolan and Lewis Semprini, cultures are being characterized that can transform cis-DCE and VC when grown on acetate, propionate, and butyrate. In addition, cultures are being isolated and characterized that can grow directly on VC. Mycobacterium strain JS60 was found to grow on acetate, propionate, and butyrate, but could not grow on formate or lactate. When acetate was used as an augmenting growth substrate, ethylene and VC utilization rates increased. When growing on acetate, strain JS60 cometabolized c-DCE and t-DCE, but not 1,1-DCE, with c-DCE transformed more rapidly than t-DCE. JS60 is able to cometabolize fluoroethene (FE) and directly grow on ethene and VC. Currently, no bacteria have been described in the literature that are capable of utilizing FE as a sole carbon and energy source, but preliminary work with Nocardioides strain JS614 shows growth on FE along with ethene and VC.

Project 2-OSU-06 (Grant No. R828772C011)is being conducted at Oregon State University under the direction of James Ingle. The goal of this study is to develop, refine, and use sensors and field instruments, based on redox indicators and other reagents, as on site, on line, or in-situ monitoring tools for assessing and optimizing redox and related conditions for treatment of PCE and TCE with dehalogenating cultures. A fiber optic probe to monitor redox status has been developed with immobilized redox indicator film at its tip. The probe is used in conjunction with a light source and CCD spectrometer to monitor the absorbance of the indicator. This probe can be easily positioned in soil columns and provides for true in-situ monitoring. Redox indicators placed on the fiber optic probe respond comparably to those installed in flow cells and connected by flow loops and allow for true in-situ monitoring. A new method has been developed to determine the “reductive capacity” (RC) or “effective concentrations of reductants” in aqueous anaerobic samples taken from microcosm bottles, soil columns, and reactors. This technique has been successful at identifying significant changes in the redox conditions in columns and microcosms (e.g., a 50% drop in RC when the column is disturbed). The column was packed with sediment from the Hanford site and inoculated with the Evanite culture. As observed during previous microcosm experiments with the Evanite culture, the indicator cresyl violet is about one-half or fully reduced at all ports indicating suitable conditions for dehalogenation. The researchers have successfully used the redox flow sensor and the fiber optic probe to monitor redox conditions in the columns studies of project 2-OSU-7, described below. The researchers anticipate the eventual automation of this measurement to allow for continuous monitoring.

Project 2-OSU-07 (Grant No. R828772C012), a joint project of Oregon State University and Stanford University, is evaluating the transformation of chlorinated ethenes in continuous-flow column studies with the Victoria Strain (VS) and the Evanite Strain (EV) cultures that have been developed and kinetically characterized in previous WRHSRC projects. Molecular methods, such as FISH and Real-Time PCR, are being used to determine the spatial distribution of the cultures and quantify the dehalogenating biomass within the columns. RNA-based methods are also being applied to determine energetically based TCE and VC-dehalogenating activity temporally and spatially within the column. A second series of column tests were initiated this year. The columns were sized so that samples could be obtained for concentration measurements a t three spatial locations along the column as well as the column exit, and so solid coupons could be collected at three different spatial locations to enumerate microbial activity. A series of transient tests have been performed with gradual increases in PCE concentration from 10 mg/L to 50 mg/L. Complete transformation of VC to ethene was achieved with a hydraulic residence time of 4.5 days, and PCE is transformed to VC and ethene within a hydraulic residence to of 1.5 days.

Relative abundance of selected genes catalyzing the stepwise dechlorination of PCE to ethene in the first column study was determined . The tracked genes are specific to subpopulations of the genus Dehalococcoides indicating functional and spatial differences in the localization of different Dehalococcoides strains along the columns vertical profile. The primer for the TCE and VC reductive dehalogenases was further used in real-time reverse transcription (RT-) PCR experiments to study gene expression along the vertical horizon of the first reactor. The results showed that RT-PCR could be used to quantify the abundance and activity of genes involved in the reductive dehalogenation of PCE under bioremediation conditions. The protocol and oligonucleotide primer developed in this study are powerful tools for monitoring intrinsic transformations and evaluating laboratory scale bioreactors and field sites undergoing bioremediation.

Project 2-SU-04 (Grant No. R828772C013), a project at Stanford University under the direction of Peter Kitanidis and Craig Criddle, is investigating novel methods for the measurement of transverse dispersion in homogeneous isotropic unconsolidated porous media. New methods were developed for the measurement of local transverse dispersion in isotropic porous media based on helical and cochlea-like devices. Experiments were performed similar to the tracer test through a laboratory column packed with a porous medium and to measure the breakthrough curve; however, the objective was not to determine the column-scale longitudinal dispersion but the transverse dispersion. The experiments showed relative advantages of each device, instrument, and methodology that were used to estimate transverse dispersivity. The most noteworthy conclusions of this research are that the results from the two devices, helix and cochlea, are in agreement and that the ratio of transverse dispersivity to longitudinal dispersivity that was estimated agrees with the higher ratios reported in the literature.

Project 2-SU-05 (Grant No. R828772C014), a project at Stanford University under the direction of Martin Reinhard, is evaluating the role of microstructure on contaminant sorption and desorption, as well as abiotic transformations. The overall goal of this project is to develop a better understanding of the impact of soil nanopores on the fate and transport of halogenated hydrocarbon contaminants. Specific project goals are to: (1) study the kinetics of slow sorption and desorption of halogenated hydrocarbons in aquifer sediment, and (2) determine the effect of sorption on contaminant reactivity. Recent work focused on sorption of TCE in zeolites with a range of hydrophobic surface properties. High silica zeolites, both partially dehydrated and wet, could sorb more TCE than the low Si/Al zeolite under the same conditions. Experimental results suggest that the density of hydrophilic centers (surface cations and hydrogen bonding sites) on the pore wall surface of micropores plays a key role in water sorption and determines their hydrophobicity. The results indicate that sorption of hydrophobic organic molecules in hydrophobic micropores occurs through displacing the weakly sorbed water molecules in them, and organic molecules co-exist with the strongly sorbed water molecules in the micropores. Their experimental data show that reactive (i.e., hydrolysable) contaminants sorbed in slow desorbing sites of geological solids react significantly slower than in bulk solution, suggesting that the contaminants reside in an environment that is to some extent, excluded from water. As a result, the halogenated hydrocarbon molecules in hydrophobic nanopores are less exposed to water molecules and are prevented from hydrolysis.

Training and Technology Transfer

Training and technology transfer activities included providing information on the Center’s activities through our Web site, the development of Research Briefs on the results of Center research projects, development of online tutorials, and presentations at conferences and workshops. Our Web site usage has continued to increase, with over 3,000 visits during the month of September 2005. Four Research Briefs, representing short summaries of Center projects, were developed to communicate our research results to practitioners and others interested in emerging cleanup technologies. The Research Briefs were advertised through our Center’s electronic newsletter, Tech Direct, EPA Region 10 Science Forum, and EPA Region 9 Hazardous Substance Technical Liaison Newsletter, as well as the National Institute of Environmental Health Sciences (NIEHS) research briefs distribution list. A tutorial was developed for simulating groundwater remediation using Interactive Groundwater Model 3.5.6, with a focus on modeling pump-and-treat. WRHSRC researchers continue to be active in conferences and workshops. For example, eight presentations were given by WRHSRC researchers at the Joint International Symposium for Subsurface Microbiology (ISSM 2005) and Environmental Biogeochemistry (ISEM XVII) in Jackson Hole, Wyoming, last August. The WRHSRC continues to train graduate students. Over the past year, two M.S. theses and three Ph.D. theses were completed on research funded by the WRHSRC.

TOSC and TAB Programs

The two outreach programs of importance are Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB). These programs are directed by Ken Williamson and Denise Lach at Oregon State University.

TOSC provides interested community groups with technical information and assistance that can enable early and meaningful public participation in decisions that affect health and welfare. The TOSC program provides a viable alternative strategy for communities that do not qualify for a Technical Assistance Grant (TAG) from the EPA. The TOSC team is comprised of university faculty and students, as well as contracted environmental professionals with specialization in environmental engineering, risk communication, public health, information transfer, environmental justice, and community relations. Currently, the TOSC program is actively working with communities in Oregon (3), Washington (2), and California (8) (the number following the state designates the number of communities in each state).

The TAB program provides assistance to communities attempting to address cleanup and redevelopment of properties whose reuse has been prevented by real or perceived contamination. TAB attempts to improve involvement of all affected parties in the cleanup and redevelopment process through education and training. The TAB program is currently working with two communities in Oregon and one in Idaho. TAB participated in the 2005 Oregon Brownfields Conference by organizing two sessions (Brownfields Basics and Meaningful Community Involvement) and making a short movie about brownfields redevelopment in three communities in Oregon.


Journal Articles: 60 Displayed | Download in RIS Format

Other center views: All 158 publications 63 publications in selected types All 60 journal articles
Type Citation Sub Project Document Sources
Journal Article Achong GR, Rodriguez AM, Spormann AM. Benzylsuccinate synthase of Azoarcus sp. strain T: cloning, sequencing, transcriptional organization, and its role in anaerobic toluene and m-xylene mineralization. Journal of Bacteriology 2001;183(23):6763-6770. R828772 (Final)
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  • Journal Article Arp DJ, Yeager CM, Hyman MR. Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene. Biodegradation 2001;12(2):81-103. R828772 (2002)
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    R825689C027 (Final)
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  • Journal Article Benekos ID, Cirpka OA, Kitanidis PK. Experimental determination of transverse dispersivity in a helix and a cochlea. Water Resources Research 2006;42:W07406, doi:10.1029/2005WR004712. R828772 (Final)
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  • Journal Article Cantrell KM, Ingle Jr JD. The SLIM spectrometer. Analytical Chemistry 2003;75(1):27-35. R828772 (2002)
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    R828772C007 (2001)
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  • Journal Article Cantrell K, Ingle Jr. JD. Design and evaluation of a membrane sampling spectrometer array for real-time, in-situ depth profiling of sub-surface waters. Aquatic Sciences-Research Across Boundaries. R828772 (2003)
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    Journal Article Cheng H, Reinhard M. Sorption of trichloroethylene in hydrophobic micropores of dealuminated Y zeolites and natural minerals. Environmental Science & Technology 2006;40(24):7694-7701. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cheng H, Reinhard M. Measuring hydrophobic micropore volumes in geosorbents from trichloroethylene desorption data. Environmental Science & Technology 2006;40(11):3595-3602. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cheng H, Reinhard M. Sorption and inhibited dehydrohalogenation of 2,2-dichloropropane in micropores of dealuminated Y zeolites. Environmental Science & Technology 2007;41(6):1934-1941. R828772 (Final)
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  • Journal Article Chu M, Kitanidis PK, McCarty PL. Effects of biomass accumulation on microbially enhanced dissolution of a PCE pool: a numerical simulation. Journal of Contaminant Hydrology 2003;65(1-2):79-100. R828772 (2002)
    R828772 (Final)
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  • Journal Article Cunningham JA, Deitsch JJ, Smith JA, Reinhard M. Quantification of contaminant sorption-desorption time-scales from batch experiments. Environmental Toxicology and Chemistry 2005;24(9):2160-2166. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cupples AM, Spormann AM, McCarty PL. Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR. Applied and Environmental Microbiology 2003;69(2):953-959. R828772 (2002)
    R828772 (Final)
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  • Journal Article Cupples AM, Spormann AM, McCarty PL. Comparative evaluation of chloroethene dechlorination to ethene by Dehalococcoides-like microorganisms. Environmental Science & Technology 2004;38(18):4768-4774. R828772 (2004)
    R828772 (Final)
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  • Journal Article Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Effects of dichloroethene isomers on the induction and activity of butane monooxygenase in the alkane-oxidizing bacterium “Pseudomonas butanovora.” Applied and Environmental Microbiology 2005;71(10):6054-6059. R828772 (2003)
    R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Product repression of alkane monooxygenase expression in Pseudomonas butanovora. Journal of Bacteriology 2006;188(7):2586-2592. R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Doughty DM, Halsey KH, Vieville CJ, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Propionate inactivation of butane monooxygenase activity in ‘Pseudomonas butanovora’: biochemical and physiological implications. Microbiology 2007;153(Pt 11):3722-3729. R828772 (Final)
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  • Journal Article Doughty DM, Kurth EG, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Evidence for involvement of copper ions and redox state in regulation of butane monooxygenase in Pseudomonas butanovora. Journal of Bacteriology 2008;190(8):2933-2938. R828772 (Final)
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  • Journal Article Doughty DM, Arp DJ, Bottomley PJ. Dichloroethylenes as substrates and inducers of butane monooxygenase in Pseudomonas butanovora. Microbiology. R828772 (2003)
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    Journal Article Dupin HJ, Kitanidis PK, McCarty PL. Pore-scale modeling of biological clogging due to aggregate expansion: a material mechanics approach. Water Resources Research 2001;37(12):2965-2979. R828772 (2002)
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  • Journal Article Dupin HJ, Kitanidis PK, McCarty PL. Simulations of two-dimensional modeling of biomass aggregate growth in network models. Water Resources Research 2001;37(12):2981-2994. R828772 (2002)
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  • Journal Article Field JA, Reed RL, Istok JD, Semprini L, Bennett P, Buscheck TE. Trichlorofluoroethene: a reactive tracer for evaluating reductive dechlorination in large-diameter permeable columns. Ground Water Monitoring & Remediation 2005;25(2):68-77. R828772 (2005)
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  • Journal Article Fienen MN, Luo J, Kitanidis PK. Semi-analytical homogeneous anisotropic capture zone delineation. Journal of Hydrology 2005;312(1-4):39-50. R828772 (2003)
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  • Journal Article Fienen MN, Luo J, Kitanidis PK. A Bayesian geostatistical transfer function approach to tracer test analysis. Water Resources Research 2006;42:W07426, doi:10.1029/2005WR004576. R828772 (Final)
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  • Journal Article Frascari D, Kim Y, Dolan ME, Semprini L. A kinetic study of aerobic propane uptake and cometabolic degradation of chloroform, cis-dichloroethylene and trichloroethylene in microcosms with groundwater/aquifer solids. Water, Air, & Soil Pollution: Focus 2003;3(3):285-298. R828772 (2002)
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  • Journal Article Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater. 1. Dynamics of a recirculating well system. Water Resources Research 2002;38(4):1039, doi:10.1029/2001WR000379. R828772 (2002)
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  • Journal Article Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater. 2. Comprehensive analysis of field data using reactive transport modeling. Water Resources Research 2002;38(4):1040, doi:10.1029/2001WR000380. R828772 (2002)
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  • Journal Article Goltz MN, Williamson KJ. Transfer and commercialisation of contaminated groundwater remediation technologies. International Journal of Technology Transfer and Commercialisation 2002;1(4):329-346. R828772 (2002)
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  • Journal Article Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Trichloroethylene degradation by butane-oxidizing bacteria causes a spectrum of toxic effects. Applied Microbiology and Biotechnology 2005;68(6):794-801. R828772 (2003)
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  • Journal Article Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Site-directed amino acid substitutions in the hydroxylase α subunit of butane monooxygenase from Pseudomonas butanovora: implications for substrates knocking at the gate. Journal of Bacteriology 2006;188(13):4962-4969. R828772 (Final)
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  • Journal Article Halsey KH, Doughty DM, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Evidence for modified mechanisms of chloroethene oxidation in Pseudomonas butanovora mutants containing single amino acid substitutions in the hydroxylase α-subunit of butane monooxygenase. Journal of Bacteriology 2007;189(14):5068-5074. R828772 (Final)
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  • Journal Article Johnson HA, Pelletier DA, Spormann AM. Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme. Journal of Bacteriology 2001;183(15):4536-4542. R828772 (2002)
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  • Journal Article Jones BD, Ingle Jr JD. Evaluation of immobilized redox indicators as reversible, in situ redox sensors for determining Fe(III)-reducing conditions in environmental samples. Talanta 2001;55(4):699-714. R828772 (2002)
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  • Journal Article Jones BD, Ingle Jr JD. Evaluation of redox indicators for determining sulfate-reducing and dechlorinating conditions. Water Research 2005;39(18):4343-4354. R828772 (Final)
    R828772C011 (2005)
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  • Journal Article Kim Y, Arp DJ, Semprini L. Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;80(5):498-508. R828772 (2002)
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  • Journal Article Kim Y, Arp DJ, Semprini L. A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1-trichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;77(5):564-576. R828772 (2002)
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  • Journal Article Kim Y, Semprini L. Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane-grown mixed culture. Water Science & Technology 2005;52(8):125-131. R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Lee IS, Bae JH, Yang Y, McCarty PL. Simulated and experimental evaluation of factors affecting the rate and extent of reductive dehalogenation of chloroethenes with glucose. Journal of Contaminant Hydrology 2004;74(1-4):313-331. R828772 (2004)
    R828772 (Final)
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  • Journal Article Luo J, Kitanidis PK. Fluid residence times within a recirculation zone created by an extraction-injection well pair. Journal of Hydrology 2004;295(1-4):149-162. R828772 (2003)
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    R828772 (Final)
    R828772C013 (2005)
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  • Journal Article Luo J, Wu W, Fienen MN, Jardine PM, Mehlhorn TL, Watson DB, Cirpka OA, Criddle CS, Kitanidis PK. A nested-cell approach for in situ remediation. Ground Water 2006;44(2):266-274. R828772 (Final)
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  • Journal Article Luo J, Cirpka OA, Kitanidis PK. Temporal-moment matching for truncated breakthrough curves for step or step-pulse injection. Advances in Water Resources 2006;29(9):1306-1313. R828772 (Final)
    R828772C013 (2005)
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  • Journal Article McMurdie PJ, Behrens SF, Holmes S, Spormann AM. Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species. Applied and Environmental Microbiology 2007;73(8):2744-2747. R828772 (Final)
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  • Journal Article Muller JA, Rosner BM, von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM. Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Applied and Environmental Microbiology 2004;70(8):4880-4888. R828772 (2004)
    R828772 (Final)
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  • Journal Article Munakata N, Reinhard M. Palladium-catalyzed aqueous hydrodehalogenation in column reactors:modeling of deactivation kinetics with sulfide and comparison of regenerants. Applied Catalysis B:Environmental 2007;75(1-2):1-10. R828772 (Final)
  • Abstract: Science Direct
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  • Journal Article Niemet MR, Semprini L. Column studies of anaerobic carbon tetrachloride biotransformation with Hanford aquifer material. Ground Water Monitoring & Remediation 2005;25(3):82-92. R828772 (2005)
    R828772 (Final)
  • Full-text: Oregon State University PDF
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  • Journal Article Pon G, Hyman MR, Semprini L. Acetylene inhibition of trichloroethene and vinyl chloride reductive dechlorination. Environmental Science & Technology 2003;37(14):3181-3188. R828772 (2003)
    R828772 (Final)
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  • Journal Article Pon G, Semprini L. Anaerobic reductive dechlorination of 1-chloro-1-fluoroethene to track the transformation of vinyl chloride. Environmental Science & Technology 2004;38(24):6803-6808. R828772 (2003)
    R828772 (2004)
    R828772 (Final)
    R828772C012 (2005)
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  • Journal Article Ruiz-Haas P, Ingle Jr JD. Monitoring redox conditions with flow-based and fiber optic sensors based on redox indicators: application to reductive dehalogenation in a bioaugmented soil column. Geomicrobiology Journal 2007;24(3-4):365-378. R828772 (Final)
  • Abstract: Informa World Abstract
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  • Journal Article Sayavedra-Soto LA, Doughty DM, Kurth EG, Bottomley PJ, Arp DJ. Product and product-independent induction of butane oxidation in Pseudomonas butanovora. FEMS Microbiology Letters 2005;250(1):111-116. R828772 (Final)
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  • Journal Article Schroth MH, Istok JD. Approximate solution for solute transport during spherical-flow push-pull tests. Ground Water 2005;43(2):280-284. R828772 (2005)
    R828772 (Final)
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  • Journal Article Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Laboratory, field, and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. Advances in Water Resources 2007;30(6-7):1528-1546. R828772 (Final)
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  • Journal Article Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. European Journal of Soil Biology 2007;43(5-6):322-327. R828772 (Final)
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  • Journal Article Skinner KM, Martinez-Prado A, Hyman MR, Williamson KJ, Ciuffetti LM. Pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, Graphium sp. Applied Microbiology and Biotechnology 2008;77(6):1359-1365. R828772 (Final)
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  • Journal Article Sriwatanapongse W, Reinhard M, Klug CA. Reductive hydrodechlorination of trichloroethylene by palladium-on-alumina catalyst:13C solid-state NMR study of surface reaction precursors. Langmuir 2006;22(9):4158-4164. R828772 (Final)
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  • Journal Article Taylor AE, Dolan ME, Bottomley PJ, Semprini L. Utilization of fluoroethene as a surrogate for aerobic vinyl chloride transformation. Environmental Science & Technology 2007;41(18):6378-6383. R828772 (Final)
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  • Journal Article Yang Y, McCarty PL. Comparison between donor substrates for biologically enhanced tetrachloroethene DNAPL dissolution. Environmental Science & Technology 2002;36(15):3400-3404. R828772 (2002)
    R828772 (Final)
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  • Journal Article Yeager CM, Bottomley PJ, Arp DJ. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Applied and Environmental Microbiology 2001;67(12):5384-5391. R828772 (2002)
    R828772 (Final)
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  • Journal Article Yeager CM, Bottomley PJ, Arp DJ. Cytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4. Applied and Environmental Microbiology 2001;67(5):2107-2115. R828772 (2002)
    R828772 (Final)
    R825689C027 (Final)
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  • Journal Article Yeager CM, Arthur KM, Bottomley PJ, Arp DJ. Trichloroethylene degradation by toluene-oxidizing bacteria grown on non-aromatic substrates. Biodegradation 2004;15(1):19-28. R828772 (2003)
    R828772 (2004)
    R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Yu S, Semprini L. Comparison of trichloroethylene reductive dehalogenation by microbial communities stimulated on silicon-based organic compounds as slow-release anaerobic substrates. Water Research 2002;36(20):4985-4996. R828772 (2002)
    R828772 (Final)
    R828772C001 (2001)
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  • Journal Article Yu S, Semprini L. Kinetics and modeling of reductive dechlorination at high PCE and TCE concentrations. Biotechnology and Bioengineering 2004;88(4):451-464. R828772 (2004)
    R828772 (Final)
  • Abstract from PubMed
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  • Journal Article Yu S, Dolan ME, Semprini L. Kinetics and inhibition of reductive dechlorination of chlorinated ethylenes by two different mixed cultures. Environmental Science & Technology 2005;39(1):195-205. R828772 (2003)
    R828772 (Final)
    R828772C012 (2005)
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  • Supplemental Keywords:

    RFA, Scientific Discipline, Air, Waste, Water, POLLUTANTS/TOXICS, air toxics, Contaminated Sediments, Environmental Chemistry, Chemicals, Brownfields, Hazardous Waste, Ecology and Ecosystems, Groundwater remediation, Hazardous, Environmental Engineering, brownfield sites, environmental hazards, sediment treatment, in situ remediation, advanced treatment technologies, NAPL, chemical wastes, air pollutants, contaminant dynamics, in situ treatment, VOCs, contaminated sediment, ambient air, palladium catalysis, contaminated soil, emissions, chemical contaminants, in-situ treatment of chlorinated solvents, treatment, remediation, atmospheric aerosols, chlorinated VOCs, contaminated groundwater, air emissions, technology transfer, VOC remediation, contaminated aquifers, NAPLs, Volatile Organic Compounds (VOCs), bioremediation, chlorinated solvents, groundwater, TCE

    Relevant Websites:

    http://wrhsrc.oregonstate.edu/ Exit
    http://tosc.oregonstate.edu/TAB/index.htm Exit
    http://tosc.oregonstate.edu/ Exit

    Progress and Final Reports:

    Original Abstract
  • 2002 Progress Report
  • 2003 Progress Report
  • 2004 Progress Report
  • 2006
  • Final Report
  • Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bio- remediation of Trichloroethylene at NAPL Source Zone Concentrations
    R828772C002 Strategies for Cost-Effective In-situ Mixing of Contaminants and Additives in Bioremediation
    R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
    R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions
    R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers
    R828772C006 Development of the Push-Pull Test to Monitor Bioaugmentation with Dehalogenating Cultures
    R828772C007 Development and Evaluation of Field Sensors for Monitoring Bioaugmentation with Anaerobic Dehalogenating Cultures for In-Situ Treatment of TCE
    R828772C008 Training and Technology Transfer
    R828772C009 Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB) Programs
    R828772C010 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
    R828772C011 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation for In Situ Treatment of PCE and TCE
    R828772C012 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity
    R828772C013 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media
    R828772C014 The Role of Micropore Structure in Contaminant Sorption and Desorption