2003 Progress Report: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil

EPA Grant Number: R828772
Center: HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils
Center Director: Semprini, Lewis
Title: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil
Investigators: Semprini, Lewis
Current Investigators: Semprini, Lewis , Reinhard, Martin , Williamson, Kenneth J.
Institution: Oregon State University , Stanford University
Current Institution: Oregon State University
EPA Project Officer: Lasat, Mitch
Project Period: September 1, 2001 through August 31, 2006 (Extended to August 31, 2007)
Project Period Covered by this Report: September 1, 2002 through August 31, 2003
Project Amount: $5,572,000
RFA: Hazardous Substance Research Centers - HSRC (2001) RFA Text |  Recipients Lists
Research Category: Hazardous Waste/Remediation , Land and Waste Management

Objective:

The Western Region Hazardous Substance Research Center (WRHSRC) is a cooperative activity between Oregon State University (OSU) and Stanford University that was established in October 2001. The Center is a continuation of the original HSRC established in 1989, to address critical hazardous substance problems in U.S. Environmental Protection Agency (EPA) Regions 9 and 10. These two Regions include the states of Alaska, Arizona, California, Hawaii, Idaho, Nevada, Oregon, and Washington, and the territory of Guam. The Center receives its base financial support from the EPA. The objectives of the Center are to: (1) develop innovative technologies for the in situ treatment of volatile organic chemicals (VOCs) in groundwater, especially chlorinated solvents; (2) increase the number, speed, and efficiency of available treatment options for both high concentration source zones and diffuse contamination plumes; and (3) disseminate the results of research to the industrial and regulatory communities to foster exchange of information with these communities and to promote a better understanding of the scientific capability to detect, assess, and mitigate risks associated with hazardous substance usage and disposal.

Groundwater cleanup and site remediation, with a strong emphasis on treatments that use microbes or chemical catalysts to transform VOCs into harmless substances, represent the major focus of Center activities. Research projects include biological (biotic), physical, and chemical (abiotic) treatment processes, as well as in situ characterization methods for monitoring the progress of both intrinsic and enhanced remediation. In combination with basic laboratory and field studies, physical and mathematical models are being used to study these processes and to provide a bridge between theory and practice.

The technology transfer program involves the process of taking new technologies from the laboratory to the field. Center researchers are working with other federal agencies, such as the Department of Defense (DoD) and the Department of Energy (DoE), and private industry in conducting field evaluations of new technologies. Technical Outreach Services for Communities (TOSC) is a technical assistance program designed to aid communities confronted with environmental contamination by hazardous waste sites. TOSC provides interested community groups with technical information and assistance that can enable early and meaningful public participation in decisions that affect health and welfare. The Center's Technical Assistance to Brownfields (TAB) Program provides assistance to communities attempting to address cleanup and redevelopment of properties whose reuse has been prevented by real or perceived contamination. TAB attempts to improve involvement of all affected parties in cleanup and redevelopment processes through education and training.

Seventeen of the 24 OSU and Stanford University faculty members involved in the Center are directing the Center's research, training, and technology transfer activities. Collectively, they represent an integrated research group of many different disciplines, including biochemistry, chemistry, environmental engineering, environmental chemistry, geosciences, hydrogeology, molecular biology, microbiology, public health, and sociology. Lewis Semprini is Director of the Center and of the research program. Kenneth J. Williamson serves as Associate Director in charge of training, technology transfer, and community outreach. Martin Reinhard, the Assistant Director, is in charge of the Center's quality control program. Garrett Jones is the Center's administrative assistant.

The Center has two major advisory groups to guide its activities. The Science Advisory Committee (SAC) has oversight for all Center research activities and technology transfer activities, and the Outreach Advisory Committee (OAC) oversees the Center's TOSC and TAB programs. The members represent federal and state governments, industry, consulting firms, and universities. Experts with a broad range of expertise are included in the SAC and the OAC.

The education of students interested in careers directed toward finding solutions to environmental problems is another important goal. Sixteen graduate students, including 14 Ph.D. students, have been supported during the second year of the Center. More than 50 percent of the Center's core funds are being directed toward the graduate training of students through the Center's research and outreach projects.

Progress Summary:

The major focus of research activities for the OSU-Stanford WRHSRC, and indeed its major mission, has been the conduct of basic research related to the in situ treatment of VOC subsurface contamination. During the past year, research has continued in seven research projects associated with the in situ remediation of chlorinated solvents. The projects and the researchers are summarized in Table 1.

Table 1. Research Project Summary

EPA Agreement No.
Title
Principal Investigator (PI), Co-PI
R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bioremediation of Trichloroethylene at Nonaqueous Phase Liquid (NAPL) Source Zone Concentrations Lewis Semprini, PI
Mark E. Dolan, Co-PI
R828772C002 Strategies for Cost-Effective In Situ Mixing of Contaminants and Additives in Bioremediation Peter K. Kitanidis, PI
Craig S. Criddle, Co-PI
R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds With Butane-Grown Microorganisms Peter Bottomley, PI
Daniel J. Arp,
Lynda Ciuffetti,
Stephen Giovannoni,
Lewis Semprini,
Ken Williamson,
Mark Dolan, Co-PIs
R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions Martin Reinhard, PI
John Westall, Co-PI
R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers Martin Reinhard, PI
R828772C006 Development of the Push-Pull Test To Monitor Bioaugmentation with Dehalogenating Cultures Mark E. Dolan, PI
Jennifer A. Field,
Jonathan D. Istok, Co-PIs
R828772C007 Development and Evaluation of Field Sensors for Monitoring Bioaugmentation With Anaerobic Dehalogenating Cultures for In Situ Treatment of TCE James D. Ingle, PI

Research projects include biological (biotic), physical, and chemical (abiotic) treatment processes, as well as in situ characterization methods for monitoring the progress of both intrinsic and enhanced remediation. Four project PIs are at OSU, and three are at Stanford University.

Project 1 (R828772C002), which is being conducted at Stanford University by Peter Kitanidis and Craig Criddle, is focused on developing strategies for cost-effective in situ mixing of contaminants and additives in bioremediation. Such methods will employ recirculation units, pairs of extraction-injection wells, sparging systems, biocurtains, and time- and space-sequenced operations. Over the past year, the researchers have focused on the design of an effective chemical delivery and mixing scheme for in situ bioremediation of uranium (VI) at Oak Ridge National Laboratory (ORNL). Mathematical models of flow, transport, and biogeochemistry have been developed, and predictions have been compared with the results of experiments and field tests. Software has been developed for: (1) the delineation of injection, extraction, and recirculation zones; (2) the efficient determination of breakthrough curves; (3) the application of travel-time methods of modeling transport; and (4) biogeochemical modeling using computer programs such as PHREEQC in conjunction with hydrogeological modeling within the MATLAB (MATrix LABoratory) computational environment.

Project 2 (R828772C001), which is being conducted at OSU by Lewis Semprini and Mark Dolan, aims at developing a mixed anaerobic culture that is effective at transforming perchloroethylene (PCE) and trichloroethylene (TCE) via halorespiration at elevated concentrations representative of those associated with nonaqueous phase liquid (NAPL) contamination. The specific objectives of this project are to: (1) develop a culture with the ability to reductively dechlorinate TCE to ethylene at very high concentrations (above 1,000 µM) and in the presence of dense NAPL (DNAPL); (2) characterize microbial growth and measure maximum substrate utilization rates and half velocity coefficients for successive dechlorinations of TCE to ethylene; and (3) characterize the microbial consortium by investigating molecular methods to evaluate the diversity of the mixed culture developed in the kinetic studies. Over the past year, kinetic parameters were determined for each step in the dehalogenation process, with two mixed cultures and a binary culture (a mixture of the two cultures) to describe the reductive dechlorination of chlorinated ethylenes. Kinetics of the inhibition of the chlorinated aliphatic hydrocarbons (CAHs) also were studied. Model simulations of the sequential transformation of PCE and TCE to vinyl chloride (VC) and ethene matched closely the results of batch kinetic experiments over a factor of 30 changes in concentration, using the independently determined kinetic parameters. Molecular characterization of the two cultures also was performed.

In Project 3 (R828772C003), which is being conducted at OSU by a number of investigators headed by Peter Bottomley and Daniel Arp, the CAH degrading properties of several individual strains of butane-oxidizing bacteria and fungi that are known to possess distinctly different butane monooxygenases are being examined. The work is directed towards the aerobic cometabolism of a broad range of CAHs and CAH mixtures.

Research on three subprojects has continued during the past year. Research by Peter Bottomley and Daniel Arp examined the CAH degrading properties of several individual strains of butane-oxidizing bacteria, Pseudomonas butanovora, Nocardioides CF8, and Mycobacterium vaccae JOB5, that are genotypically distinct from each other, and that are known to possess distinctly different butane monooxygenases (BMO). Their studies focused on the inactivation of the BMO resulting from transformation of different CAHs, as well as the potential from BMO induction upon exposure to CAHs.

Research by Lewis Semprini and Mark Dolan is evaluating 1,1,1-trichloroethane (TCA) and 1,1-dichloroethene (DCE) cometabolism by a Rhodocococcus sp. that has been bioaugmented into the continuous-flow, laboratory-column, packed aquifer solids from the Moffett Air Field In-Situ Test Facility. Studies have shown effective and long-term removal of 1,1,1-TCA in the column upon bioaugmentation and growth butane. Molecular-based polymerase chain reaction (PCR) probes have detected the bioaugmented microorganism in the column groundwater effluent after bioaugmentation and throughout the column test.

Studies directed by Ken Williamson and Lynda Ciuffetti are evaluating the ability of a fungi, Graphium sp., to degrade a range of VOCs, including CAHs, trichloromethanes, and polyaromatic hydrocarbons (PAHs). The study also aims to demonstrate that these reactions are catalyzed by an alkane inducible cytochrome P450 monooxygenase through heterologous expression assays with yeast. The investigators are using cloning techniques to study the P450 monooxygenase in different types of fungi. In another related project, supported by a U.S. EPA STAR fellowship, the alkane monooxygenase activity will be conferred to plants, and the kinetics and fate of environmentally significant compounds (fuel oxygenates, chlorinated solvents, and PAHs) in these transgenic plants will be determined.

Project 4 (R828772C004) is an investigation of the chemical, physical, and biological processes at the surface of palladium catalysts under groundwater treatment conditions by Martin Reinhard at Stanford University and John Westall at OSU. This project approaches optimization of the abiotic process for CAH reduction using Pd catalysts. The project aims at obtaining a thorough understanding of changes in the catalyst surface during treatment and correlating these to changes in catalytic activity. Research conducted during the second year of this project demonstrated that deactivation of the catalyst was consistent with sulfide poisoning. Treatment with sodium hypochlorite was able to fully regenerate the catalyst. The research is being undertaken in collaboration with a field study at Edwards Air Force Base (EAFB) near Lancaster, CA.

In project 5 (R828772C005), conducted by Martin Reinhard at Stanford University, the effects of sorbent microporosity on multicomponent fate and transport in contaminated groundwater aquifers, are being studied. This project is investigating the importance of one of the most fundamental processes of organic sequestration on porous sorbents—micropore sorption. The impacts of the environmental variables affecting micropore sequestration are being quantified. The competitive sorption/desorption of multiple contaminants on the natural soils is being studied to elucidate the interactions among molecules with different properties during micropore sequestration. The kinetics of contaminant uptake and release from micropores are being measured and compared with other sorption/desorption pathways. Over the past year, an apparatus has been developed specifically for measuring slow sorption and desorption kinetics of VOCs on solid materials packed in columns. This design has expanded the investigative capabilities in several ways: (1) data are acquired in real-time with high resolution over the entire contaminant desorption profile; (2) contaminant detection is extremely sensitive; and (3) sorption and desorption of multiple volatile organic contaminants can be studied. The initial experimental results suggest that the property of micropores in geosorbents, rather than the total volume of the micropores, plays a key role in controlling contaminant sequestration and desorption.

In Project 6 (R828772C006), conducted by Mark Dolan, Jennifer Field, and Jonathan Istok at OSU, the push-pull test to monitor the bioaugmentation with dehalogenating cultures is being developed. The overall goal is to modify the single-well, push-pull groundwater test as a means for obtaining quantitative information on in situ dechlorinating activity before and after bioaugmentation. Two cultures characterized in Project 2 (Evanite and Point Mugu) that transform TCE to ethene are being used in this study. The transport of the culture(s) is being determined during injection into anaerobic physical aquifer models (PAMs). Spatial distributions of dechlorinating activity and redox are determined from a suite of assays conducted at sampling ports and at the injection/extraction well. Push-pull tests are being conducted at the injection/extraction well to assess changes in reductive dechlorination activity resulting from bioaugmentation.

The investigators currently are evaluating the survivability of the cultures in groundwater/sediment microcosms and studying basic transport behavior in columns. Molecular methods, using Dehalococcoides sp., group-specific PCR primers are being used to track the dehalogenators. Serial dilutions of the Evanite culture were extracted and analyzed using Dehalococcoides group-specific PCR, and dilutions down to 10-4 were detectible by this process. Future work includes expanding this testing to real-time, quantifiable PCR analyses to attain better enumeration of Dehalococcoides sp.

In Project 7 (R828772C007), directed by James Ingle at OSU, field sensors are being developed and evaluated for determining redox conditions during in situ treatment of TCE. This study aims to refine and use redox sensors based on redox indicators as monitoring tools for assessing and optimizing redox conditions for treatment of TCE and PCE with dehalogenating cultures. Flow sensors based on redox indicators are being deployed in two primary collaborative situations for calibration and demonstration of their applicability: (1) continuous monitoring of redox conditions of cultures inside bioreactors or microcosm bottles as a tool for the optimizing conditions for effective dechlorination of PCE or TCE with enriched halorespiratory cultures; and (2) online monitoring of the redox status of the material in a PAM bioaugmented with the developed dehalogenating cultures.

Research in the second year evaluated the dechlorinating culture (Project 2) in bioreactors and microcosm bottles to calibrate the response of the redox indicators to the dechlorination of PCE. The indicator data support the concept that the dechlorinating process is increasingly reduced as PCE is dechlorinated, with the most reducing step in the process being the dechlorination of VC to ethene. Research also has focused on the development of a hydrogen sensor on membranes with platinum embedded membranes.

Training and Technology Transfer

The education of graduate students in the research focus area of the Center is one of our main training activities. Two M.S. students and 14 Ph.D. students received Center funds through graduate research assistantships over the past year. Two of the students have been funded through the Center outreach program and 14 through the different research projects. Through Center funding, students are being trained to do fundamental research at the Ph.D. level in a broad range of disciplines. More than one-half of the Center funding is devoted to the training of graduate students, with the funding directly assigned to tuition, stipends, travel to conferences, and supplies and materials for research.

Technology transfer is an important component of the WRHSRC. The goals of the training and technology transfer program are to: (1) promote teamwork and information exchange among researchers using Web pages and seminars; (2) provide information transfer with practitioners using Web pages, electronic newsletter, video workshops, and faculty presentations and publications; (3) test new technologies through pilot-scale testing and developing online project databases; and (4) implement full-scale demonstration projects. The WRHSRC Web Site, developed and maintained at OSU, receives about 700 visitors per month. Some of the information contained on the Web site includes descriptions of research focus areas and projects; a database of WRHSRC publications and previous projects, 1989-2002; descriptions of Center outreach programs and links to the separate Web sites for the Western Region TOSC/TAB programs; and a News and Events page with regular postings. The Web site features Center publications and a searching capability, research briefs, demonstration projects for TOSC, and a page that assists clients through the process of obtaining help from TOSC. Interested clients and individuals can subscribe to the new e-mail newsletter (launched in the spring of 2003) for WRHSRC and TOSC (started in the fall of 2002). Three Research Briefs, focusing on the Center Research Projects, were distributed through e-mail in 2003.

The WRHSRC also houses a program to promote training activities related to lead paint contamination and disposal. The Western Regional Lead Training Center at OSU (WRLTC-OSU), originally established with U.S. EPA grant funding in 1992, is an accredited, nonprofit training provider of lead-based paint abatement workshops for the U.S. EPA and the State of Oregon certification programs. It is the only Oregon-accredited, lead-based paint (LBP) training center and provides all of the federal LBP curricula. WRLTC-OSU also is accredited in Washington, Alaska, and Idaho, as well as in all of the Pacific Northwest Indian Tribes. In 2003, more than 200 students attended 22 workshops and received 242 certificates. Accredited workshops included Lead Inspector, Lead Risk Assessor, Combined Lead Inspector and Risk Assessor Refresher, Lead Abatement Worker, Lead Abatement Supervisor, Combined Lead Abatement Worker and Supervisor Refresher, and Lead Project Designer.

TOSC and TAB Programs

The two outreach programs of importance are TOSC and TAB. These programs are directed by Ken Williamson and Denise Lach at OSU.

TOSC provides interested community groups with technical information and assistance that can enable early and meaningful public participation in decisions that affect health and welfare. The TOSC Program provides a viable alternative strategy for communities that do not qualify for a Technical Assistance Grant from the U.S. EPA. The TOSC team is comprised of university faculty and students as well as contracted environmental professionals with specialization in environmental engineering, risk communication, public health, information transfer, environmental justice, and community relations. Currently, the TOSC Program is actively working with communities in Oregon (2), Washington (1), Arizona (2), and California (12) (the number following the state designates the number of communities in each state).

The TAB program provides assistance to communities attempting to address cleanup and redevelopment of properties whose reuse has been prevented by real or perceived contamination. TAB attempts to improve involvement of all affected parties in the cleanup and redevelopment processes through education and training. The TAB program currently is working in Oregon, Washington, California, and Nevada. TAB has worked with the cities of Portland, OR; Spokane, WA; East Palo Alto, CA; and Richmond, CA, on various issues related to brownfields redevelopment. The TAB Program also helps coordinate an annual brownfields conference in partnership with the Oregon Department of Environmental Quality and the Oregon Economic and Community Development Department.

Center Annual Research Meeting

In August 2003, Center researchers, graduate students, outreach specialists, and SAC and OAC members met for the second annual meeting of the WRHSRC. The meeting goal was to discuss the progress of the Center's research projects over the past year, and for the SAC to review and rank new proposals for the third and fourth years of center funding. In the morning and afternoon, researchers from OSU and Stanford University gave presentations highlighting five of the Center's research projects and two community outreach programs. A student poster session on the Center's research projects and outreach programs was held in the afternoon. On the second day of the meeting, the SAC and OAC reviewed the progress of the Center's research and outreach programs. The SAC also reviewed research proposals for Center funding. The SAC ranked five new Center projects (discussed later) for funding.

Future Activities:

In June 2003, a Request for Proposals (RFP) was issued for research projects for the third and fourth years of Center funding. Seven proposals were submitted. The SAC reviewed, discussed, and ranked the proposals during the annual meeting in August. Based on the recommendations of the SAC panel, the following projects will be funded for the third and fourth years of the Center:

1. Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms That Grow on Organic Acids and Alcohols. Peter Bottomley, PI, OSU; Daniel Arp, Mark Dolan, Lewis Semprini, Co-PIs, OSU

2. Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation After Bioaugmentation. James Ingle, PI, OSU

3. Continuous-Flow Column Studies of Reductive Dehalogenation With Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity. Lewis Semprini, PI, OSU; Mark Dolan, Co-PI, OSU; Alfred Spormann, Co-PI, Stanford University

4. Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media. Peter K. Kitanidis, PI, Stanford University; Craig Criddle, Co-PI, Stanford University

5. The Role of Micropore Structure in Contaminant Sorption and Desorption. Martin Reinhard, PI, Stanford University.

Projects 1 and 3 deal with intrinsic and enhanced bioremediation of chlorinated solvents under aerobic and anaerobic conditions. Project 2 involves developing field methods for site characterization. The field redox sensors being developed in this project also will be used in Project 3. The final two projects deal with important transport processes of dispersion and sorption: Project 4 will investigate transport and dispersive mixing, whereas Project 5 will investigate mechanisms for slow sorption and desorption from aquifer materials.


Journal Articles: 60 Displayed | Download in RIS Format

Other center views: All 158 publications 63 publications in selected types All 60 journal articles
Type Citation Sub Project Document Sources
Journal Article Achong GR, Rodriguez AM, Spormann AM. Benzylsuccinate synthase of Azoarcus sp. strain T: cloning, sequencing, transcriptional organization, and its role in anaerobic toluene and m-xylene mineralization. Journal of Bacteriology 2001;183(23):6763-6770. R828772 (Final)
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  • Journal Article Arp DJ, Yeager CM, Hyman MR. Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene. Biodegradation 2001;12(2):81-103. R828772 (2002)
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    R825689C027 (Final)
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  • Journal Article Benekos ID, Cirpka OA, Kitanidis PK. Experimental determination of transverse dispersivity in a helix and a cochlea. Water Resources Research 2006;42:W07406, doi:10.1029/2005WR004712. R828772 (Final)
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  • Journal Article Cantrell KM, Ingle Jr JD. The SLIM spectrometer. Analytical Chemistry 2003;75(1):27-35. R828772 (2002)
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    R828772C007 (2001)
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  • Journal Article Cantrell K, Ingle Jr. JD. Design and evaluation of a membrane sampling spectrometer array for real-time, in-situ depth profiling of sub-surface waters. Aquatic Sciences-Research Across Boundaries. R828772 (2003)
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    Journal Article Cheng H, Reinhard M. Sorption of trichloroethylene in hydrophobic micropores of dealuminated Y zeolites and natural minerals. Environmental Science & Technology 2006;40(24):7694-7701. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cheng H, Reinhard M. Measuring hydrophobic micropore volumes in geosorbents from trichloroethylene desorption data. Environmental Science & Technology 2006;40(11):3595-3602. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cheng H, Reinhard M. Sorption and inhibited dehydrohalogenation of 2,2-dichloropropane in micropores of dealuminated Y zeolites. Environmental Science & Technology 2007;41(6):1934-1941. R828772 (Final)
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  • Journal Article Chu M, Kitanidis PK, McCarty PL. Effects of biomass accumulation on microbially enhanced dissolution of a PCE pool: a numerical simulation. Journal of Contaminant Hydrology 2003;65(1-2):79-100. R828772 (2002)
    R828772 (Final)
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  • Journal Article Cunningham JA, Deitsch JJ, Smith JA, Reinhard M. Quantification of contaminant sorption-desorption time-scales from batch experiments. Environmental Toxicology and Chemistry 2005;24(9):2160-2166. R828772 (Final)
    R828772C014 (2005)
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  • Journal Article Cupples AM, Spormann AM, McCarty PL. Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR. Applied and Environmental Microbiology 2003;69(2):953-959. R828772 (2002)
    R828772 (Final)
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  • Journal Article Cupples AM, Spormann AM, McCarty PL. Comparative evaluation of chloroethene dechlorination to ethene by Dehalococcoides-like microorganisms. Environmental Science & Technology 2004;38(18):4768-4774. R828772 (2004)
    R828772 (Final)
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  • Journal Article Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Effects of dichloroethene isomers on the induction and activity of butane monooxygenase in the alkane-oxidizing bacterium “Pseudomonas butanovora.” Applied and Environmental Microbiology 2005;71(10):6054-6059. R828772 (2003)
    R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Product repression of alkane monooxygenase expression in Pseudomonas butanovora. Journal of Bacteriology 2006;188(7):2586-2592. R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Doughty DM, Halsey KH, Vieville CJ, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Propionate inactivation of butane monooxygenase activity in ‘Pseudomonas butanovora’: biochemical and physiological implications. Microbiology 2007;153(Pt 11):3722-3729. R828772 (Final)
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  • Journal Article Doughty DM, Kurth EG, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Evidence for involvement of copper ions and redox state in regulation of butane monooxygenase in Pseudomonas butanovora. Journal of Bacteriology 2008;190(8):2933-2938. R828772 (Final)
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  • Journal Article Doughty DM, Arp DJ, Bottomley PJ. Dichloroethylenes as substrates and inducers of butane monooxygenase in Pseudomonas butanovora. Microbiology. R828772 (2003)
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    Journal Article Dupin HJ, Kitanidis PK, McCarty PL. Pore-scale modeling of biological clogging due to aggregate expansion: a material mechanics approach. Water Resources Research 2001;37(12):2965-2979. R828772 (2002)
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  • Journal Article Dupin HJ, Kitanidis PK, McCarty PL. Simulations of two-dimensional modeling of biomass aggregate growth in network models. Water Resources Research 2001;37(12):2981-2994. R828772 (2002)
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  • Journal Article Field JA, Reed RL, Istok JD, Semprini L, Bennett P, Buscheck TE. Trichlorofluoroethene: a reactive tracer for evaluating reductive dechlorination in large-diameter permeable columns. Ground Water Monitoring & Remediation 2005;25(2):68-77. R828772 (2005)
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  • Journal Article Fienen MN, Luo J, Kitanidis PK. Semi-analytical homogeneous anisotropic capture zone delineation. Journal of Hydrology 2005;312(1-4):39-50. R828772 (2003)
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  • Journal Article Fienen MN, Luo J, Kitanidis PK. A Bayesian geostatistical transfer function approach to tracer test analysis. Water Resources Research 2006;42:W07426, doi:10.1029/2005WR004576. R828772 (Final)
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  • Journal Article Frascari D, Kim Y, Dolan ME, Semprini L. A kinetic study of aerobic propane uptake and cometabolic degradation of chloroform, cis-dichloroethylene and trichloroethylene in microcosms with groundwater/aquifer solids. Water, Air, & Soil Pollution: Focus 2003;3(3):285-298. R828772 (2002)
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  • Journal Article Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater. 1. Dynamics of a recirculating well system. Water Resources Research 2002;38(4):1039, doi:10.1029/2001WR000379. R828772 (2002)
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  • Journal Article Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater. 2. Comprehensive analysis of field data using reactive transport modeling. Water Resources Research 2002;38(4):1040, doi:10.1029/2001WR000380. R828772 (2002)
    R828772 (Final)
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  • Journal Article Goltz MN, Williamson KJ. Transfer and commercialisation of contaminated groundwater remediation technologies. International Journal of Technology Transfer and Commercialisation 2002;1(4):329-346. R828772 (2002)
    R828772 (Final)
  • Abstract: InderScience Publishers Abstract
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  • Journal Article Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Trichloroethylene degradation by butane-oxidizing bacteria causes a spectrum of toxic effects. Applied Microbiology and Biotechnology 2005;68(6):794-801. R828772 (2003)
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    R828772C010 (2005)
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  • Journal Article Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Site-directed amino acid substitutions in the hydroxylase α subunit of butane monooxygenase from Pseudomonas butanovora: implications for substrates knocking at the gate. Journal of Bacteriology 2006;188(13):4962-4969. R828772 (Final)
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  • Journal Article Halsey KH, Doughty DM, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Evidence for modified mechanisms of chloroethene oxidation in Pseudomonas butanovora mutants containing single amino acid substitutions in the hydroxylase α-subunit of butane monooxygenase. Journal of Bacteriology 2007;189(14):5068-5074. R828772 (Final)
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  • Journal Article Johnson HA, Pelletier DA, Spormann AM. Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme. Journal of Bacteriology 2001;183(15):4536-4542. R828772 (2002)
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    R825689C092 (Final)
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  • Journal Article Jones BD, Ingle Jr JD. Evaluation of immobilized redox indicators as reversible, in situ redox sensors for determining Fe(III)-reducing conditions in environmental samples. Talanta 2001;55(4):699-714. R828772 (2002)
    R828772 (Final)
  • Full-text: Science Direct Full Text
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  • Journal Article Jones BD, Ingle Jr JD. Evaluation of redox indicators for determining sulfate-reducing and dechlorinating conditions. Water Research 2005;39(18):4343-4354. R828772 (Final)
    R828772C011 (2005)
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  • Journal Article Kim Y, Arp DJ, Semprini L. Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;80(5):498-508. R828772 (2002)
    R828772 (Final)
    R828772C003 (2002)
  • Abstract from PubMed
  • Full-text: Oregon State University-PDF
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  • Journal Article Kim Y, Arp DJ, Semprini L. A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1-trichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;77(5):564-576. R828772 (2002)
    R828772 (Final)
    R828772C003 (2002)
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  • Journal Article Kim Y, Semprini L. Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane-grown mixed culture. Water Science & Technology 2005;52(8):125-131. R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Lee IS, Bae JH, Yang Y, McCarty PL. Simulated and experimental evaluation of factors affecting the rate and extent of reductive dehalogenation of chloroethenes with glucose. Journal of Contaminant Hydrology 2004;74(1-4):313-331. R828772 (2004)
    R828772 (Final)
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  • Journal Article Luo J, Kitanidis PK. Fluid residence times within a recirculation zone created by an extraction-injection well pair. Journal of Hydrology 2004;295(1-4):149-162. R828772 (2003)
    R828772 (2004)
    R828772 (Final)
    R828772C013 (2005)
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  • Journal Article Luo J, Wu W, Fienen MN, Jardine PM, Mehlhorn TL, Watson DB, Cirpka OA, Criddle CS, Kitanidis PK. A nested-cell approach for in situ remediation. Ground Water 2006;44(2):266-274. R828772 (Final)
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  • Journal Article Luo J, Cirpka OA, Kitanidis PK. Temporal-moment matching for truncated breakthrough curves for step or step-pulse injection. Advances in Water Resources 2006;29(9):1306-1313. R828772 (Final)
    R828772C013 (2005)
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  • Journal Article McMurdie PJ, Behrens SF, Holmes S, Spormann AM. Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species. Applied and Environmental Microbiology 2007;73(8):2744-2747. R828772 (Final)
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  • Journal Article Muller JA, Rosner BM, von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM. Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Applied and Environmental Microbiology 2004;70(8):4880-4888. R828772 (2004)
    R828772 (Final)
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  • Journal Article Munakata N, Reinhard M. Palladium-catalyzed aqueous hydrodehalogenation in column reactors:modeling of deactivation kinetics with sulfide and comparison of regenerants. Applied Catalysis B:Environmental 2007;75(1-2):1-10. R828772 (Final)
  • Abstract: Science Direct
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  • Journal Article Niemet MR, Semprini L. Column studies of anaerobic carbon tetrachloride biotransformation with Hanford aquifer material. Ground Water Monitoring & Remediation 2005;25(3):82-92. R828772 (2005)
    R828772 (Final)
  • Full-text: Oregon State University PDF
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  • Abstract: Wiley
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  • Journal Article Pon G, Hyman MR, Semprini L. Acetylene inhibition of trichloroethene and vinyl chloride reductive dechlorination. Environmental Science & Technology 2003;37(14):3181-3188. R828772 (2003)
    R828772 (Final)
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  • Journal Article Pon G, Semprini L. Anaerobic reductive dechlorination of 1-chloro-1-fluoroethene to track the transformation of vinyl chloride. Environmental Science & Technology 2004;38(24):6803-6808. R828772 (2003)
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    R828772 (Final)
    R828772C012 (2005)
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  • Full-text: ACS Publications HTML
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  • Journal Article Ruiz-Haas P, Ingle Jr JD. Monitoring redox conditions with flow-based and fiber optic sensors based on redox indicators: application to reductive dehalogenation in a bioaugmented soil column. Geomicrobiology Journal 2007;24(3-4):365-378. R828772 (Final)
  • Abstract: Informa World Abstract
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  • Journal Article Sayavedra-Soto LA, Doughty DM, Kurth EG, Bottomley PJ, Arp DJ. Product and product-independent induction of butane oxidation in Pseudomonas butanovora. FEMS Microbiology Letters 2005;250(1):111-116. R828772 (Final)
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  • Journal Article Schroth MH, Istok JD. Approximate solution for solute transport during spherical-flow push-pull tests. Ground Water 2005;43(2):280-284. R828772 (2005)
    R828772 (Final)
  • Abstract from PubMed
  • Full-text: Oregon State University PDF
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  • Journal Article Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Laboratory, field, and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. Advances in Water Resources 2007;30(6-7):1528-1546. R828772 (Final)
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  • Journal Article Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. European Journal of Soil Biology 2007;43(5-6):322-327. R828772 (Final)
  • Abstract: Science Direct Abstract
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  • Journal Article Skinner KM, Martinez-Prado A, Hyman MR, Williamson KJ, Ciuffetti LM. Pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, Graphium sp. Applied Microbiology and Biotechnology 2008;77(6):1359-1365. R828772 (Final)
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  • Journal Article Sriwatanapongse W, Reinhard M, Klug CA. Reductive hydrodechlorination of trichloroethylene by palladium-on-alumina catalyst:13C solid-state NMR study of surface reaction precursors. Langmuir 2006;22(9):4158-4164. R828772 (Final)
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  • Journal Article Taylor AE, Dolan ME, Bottomley PJ, Semprini L. Utilization of fluoroethene as a surrogate for aerobic vinyl chloride transformation. Environmental Science & Technology 2007;41(18):6378-6383. R828772 (Final)
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  • Journal Article Yang Y, McCarty PL. Comparison between donor substrates for biologically enhanced tetrachloroethene DNAPL dissolution. Environmental Science & Technology 2002;36(15):3400-3404. R828772 (2002)
    R828772 (Final)
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  • Journal Article Yeager CM, Bottomley PJ, Arp DJ. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Applied and Environmental Microbiology 2001;67(12):5384-5391. R828772 (2002)
    R828772 (Final)
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  • Journal Article Yeager CM, Bottomley PJ, Arp DJ. Cytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4. Applied and Environmental Microbiology 2001;67(5):2107-2115. R828772 (2002)
    R828772 (Final)
    R825689C027 (Final)
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  • Journal Article Yeager CM, Arthur KM, Bottomley PJ, Arp DJ. Trichloroethylene degradation by toluene-oxidizing bacteria grown on non-aromatic substrates. Biodegradation 2004;15(1):19-28. R828772 (2003)
    R828772 (2004)
    R828772 (Final)
    R828772C010 (2005)
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  • Journal Article Yu S, Semprini L. Comparison of trichloroethylene reductive dehalogenation by microbial communities stimulated on silicon-based organic compounds as slow-release anaerobic substrates. Water Research 2002;36(20):4985-4996. R828772 (2002)
    R828772 (Final)
    R828772C001 (2001)
  • Abstract from PubMed
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  • Journal Article Yu S, Semprini L. Kinetics and modeling of reductive dechlorination at high PCE and TCE concentrations. Biotechnology and Bioengineering 2004;88(4):451-464. R828772 (2004)
    R828772 (Final)
  • Abstract from PubMed
  • Full-text: Oregon State PDF
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  • Journal Article Yu S, Dolan ME, Semprini L. Kinetics and inhibition of reductive dechlorination of chlorinated ethylenes by two different mixed cultures. Environmental Science & Technology 2005;39(1):195-205. R828772 (2003)
    R828772 (Final)
    R828772C012 (2005)
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  • Supplemental Keywords:

    groundwater, soil, in situ, remediation, volatile organic compounds, VOCs, characterization, waste water, brownfields, TOSC, TAB, contaminated sediments, ecology, ecosystems, environmental engineering, hazardous, hazardous waste, health risk assessment, air toxics, non-aqueous phase liquid, NAPL, DNAPL, perchloroethylene, PCE, trichloroethylene, TCE, vinyl chloride, VC, advanced treatment technologies, air emissions, air pollutants, ambient air, aquifer remediation, atmospheric aerosols, bioremediation, chemical contaminants, chemical wastes, chlorinated solvents, contaminated aquifers, contaminated groundwater, contaminated soil, emissions, environmental hazards, groundwater, remediation, in situ treatment of chlorinated solvents, palladium catalysis, sediment treatment, technology transfer., RFA, Scientific Discipline, Air, Waste, Water, POLLUTANTS/TOXICS, air toxics, Contaminated Sediments, Environmental Chemistry, Chemicals, Brownfields, Hazardous Waste, Ecology and Ecosystems, Groundwater remediation, Hazardous, Environmental Engineering, brownfield sites, environmental hazards, sediment treatment, in situ remediation, advanced treatment technologies, NAPL, chemical wastes, air pollutants, contaminant dynamics, in situ treatment, VOCs, contaminated sediment, ambient air, palladium catalysis, contaminated soil, emissions, chemical contaminants, in-situ treatment of chlorinated solvents, treatment, remediation, atmospheric aerosols, chlorinated VOCs, contaminated groundwater, air emissions, technology transfer, VOC remediation, contaminated aquifers, NAPLs, Volatile Organic Compounds (VOCs), bioremediation, chlorinated solvents, groundwater, TCE

    Relevant Websites:

    http://wrhsrc.oregonstate.edu/ Exit

    Progress and Final Reports:

    Original Abstract
  • 2002 Progress Report
  • 2004 Progress Report
  • 2005 Progress Report
  • 2006
  • Final Report
  • Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
    R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bio- remediation of Trichloroethylene at NAPL Source Zone Concentrations
    R828772C002 Strategies for Cost-Effective In-situ Mixing of Contaminants and Additives in Bioremediation
    R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
    R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions
    R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers
    R828772C006 Development of the Push-Pull Test to Monitor Bioaugmentation with Dehalogenating Cultures
    R828772C007 Development and Evaluation of Field Sensors for Monitoring Bioaugmentation with Anaerobic Dehalogenating Cultures for In-Situ Treatment of TCE
    R828772C008 Training and Technology Transfer
    R828772C009 Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB) Programs
    R828772C010 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
    R828772C011 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation for In Situ Treatment of PCE and TCE
    R828772C012 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity
    R828772C013 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media
    R828772C014 The Role of Micropore Structure in Contaminant Sorption and Desorption