You are here:
MYSID TWO-GENERATION TEST GUIDELINE
Citation:
McKenney Jr., C L. MYSID TWO-GENERATION TEST GUIDELINE. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-13/011, 2006.
Impact/Purpose:
The draft proposed new mysid two-generation test guideline (OECD 2004a) describes the recommended protocol to be used. T
Description:
McKenney, Charles L., Jr. In press. Mysid Two-Generation Test Guideline. OECD Expert Group on Invertebrate Testing for Endocrine Disruptors, Organisation for Economic Co-operation and Development, Paris, France. 17 p. (ERL,GB 1215).
This guideline describes a two-generation test with an estuarine mysid that considers reproductive fitness in parents and offspring as an integrated measure of toxicant exposure. It also enables measurement of a suite of other endpoints that allow for diagnostic and definitive evaluation of endocrine disrupting chemicals (EDCs) or other types of reproductive toxicants (Verslycke et al 2004). This guideline is intended to be applicable to the mysid (Americamysis bahia), but could be adapted to other mysid species. The mysid two-generation test is a relatively long-term (normally 60-d or longer) assay that assesses early development, growth, and reproduction in two generations (F0 and F1). The two-generation test described in this guideline is an extension of existing standard practice for conducting a mysid life-cycle test (ASTM 1997, McKenney 1986, 1998 and Nimmo et al 1977, 1978).