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FACTORS INFLUENCING IN VITRO KILLING OF BACTERIA BY HEMOCYTES OF THE EASTERN OYSTER (CRASSOSTREA VIRGINICA)
Citation:
Genthner, F J., A. K. Volety, L M. Oliver, AND W S. Fisher. FACTORS INFLUENCING IN VITRO KILLING OF BACTERIA BY HEMOCYTES OF THE EASTERN OYSTER (CRASSOSTREA VIRGINICA). Presented at National Shellfisheries Association Annual Meeting, Halifax, Nova Scotia, 18-22 April 1999.
Description:
Vibrio parahaemolyticus strains altered in motility or colonial morphology (opaque versus translucent), Listeria monocytogenes mutants lacking catalase, superoxide dismutase, hemolysin, or phospholipase activities, and Vibrio vulnificus strains, possessing and lacking capsules were exposed to oyster hemocytes. Tetrazolizum dye reduction was used to quantify bacterial killing by the hemocytes. Higher killing by hemocytes was observed in summer than winter. Listeria monocytogenes was more resistant to the bactericidal activity of hemocytes than V. parahaemolyticus or V. vulnificus. No differences in hemocyte killing were observed between the different L. monocytogenes mutants. Translucent strains of V. parahaemolyticus showed higher susceptibility to killing by hemocytes than the parental opaque strain. No significant differences in killing by hemocytes were observed between encapsulated and nonencapsulated pairs of V. vulnificus. No seasonal differences (winter versus summer) were observed in activities of 19 hydrolytic enzymes associated with hemocytes.