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DIFFERENTIAL GENE EXPRESSION INDUCED BY RESPIRATORY SYNCTIAL VIRUS IN HUMAN BRONCHIAL EPITHELIAL CELLS
Citation:
Soukup, J M., M. C. Yen, C. H. Liang, J. Yuen, AND Huang, YuhChin T. DIFFERENTIAL GENE EXPRESSION INDUCED BY RESPIRATORY SYNCTIAL VIRUS IN HUMAN BRONCHIAL EPITHELIAL CELLS. Presented at American Thoracic Society Annual Conference, San Diego, CA, March 20 - 25, 2005.
Description:
Respiratory syncytial virus (RSV), a negative-stranded RNA virus, is a common viral pathogen for respiratory infection in both children and immunocompromised adults. Early host defense may play a critical role in determining the severity of the infection. To gain further insight into host defense during early RSV infection, we compared gene expression patterns in primary human bronchial epithelial cells among 0, 4 hours and 24 hours post-RSV infection (multiplicity of infection = 1) (n = 4 each). RNAs were extracted for microarray analysis and hybridized to the Affymetrix Human 133A microarrays. Differentially expressed genes were identified using the ANOVA (p<0.01) (MEV v3.0, The Institute of Genomic Research). The genes were uploaded to the Database for Annotation, Visualization and Integrated Discovery (http://appsl.niaid,nih,gov/david) to identify specific biological processes. A total of 495 genes were identified by ANOVA. Besides genes related to transcription, many genes were associated with membrane transport, cell adhesion and actin and microtubule cytoskeleton. The differentially expressed genes at 24 hours were also identified by the unpaired t-test (P < 0.01) and a ratio cut-off of > 1.5 or < 0.5, and compared to results from additional experiments with Agilent human 1A(v1) microarrays (n = 2). This latter analysis identified 466 upregulated and 330 downregulated genes. 24 upregulated genes were involved in the defense response and included genes such as granulocyte macrophage stimulating factor (CSF2), interleukin-8 (IL-8) and RANTES (CCL5), whose translated proteins were increased by RSV. These results provided a platform for generating hypothesis for the pathogenesis of RSV infection, and indicated that expression of genes related to membrane transport, cell adhesion and cytoskeleton may play an important role (This is a proposed presentation and does not necessarily reflect EPA policy).