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SPECIATION OF ARSENIC ANIMAL FEED ADDITIVES BY MICROBORE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY
Citation:
Pergantis, S. A., E. Heithmar, AND T. Hinners. SPECIATION OF ARSENIC ANIMAL FEED ADDITIVES BY MICROBORE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY. Analyst 122(10):1063-1068, (1997).
Description:
Phenylarsonic compounds have been used as poultry and swine feed additives for the purpose of growth promotion and disease prevention. Owing to the lack of suitable analytical methods, however, knowledge of their metabolism, environmental fate and impact remains incomplete. In order to compensate for this, analytical procedures were developed that allow the speciation of arsenic animal feed additives by using microbore high-performance liquid chromatography (uHPLC) coupled on-line with ICP-MS. More specifically, reversed-phase (RP) chromatographic methods were optimised to achieve the separation of various phenylarsonic acids from each other and from the more toxic inorganic arsenic compounds. This mode of chromatography eliminates such shortcomings by minimising the co-elution of arsenic species. In general, the uHPLC-ICP-MS methods developed in this study provide high selectivity, extremely good sensitivity, low limits of detection (low-ppb or sub-pg amounts of As), require small sample volumes (<1 ul), minimise waste and operate most efficiently under low mobile-phase flow rates (15-40 ul min -1), which aare compatible for use with other types of mass spectrometers, e.g., electrospray. Reference materials containing naturally occurring arsenic compounds were spiked with phenylarsonic compounds and then analysed by using the procedures developed in this study.