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ELECTROPHORETIC MOBILITY OF MYCOBACTERIUM AVIUM COMPLEX ORGANISMS
Citation:
Lytle, D A., C Frietch, AND T C. Covert. ELECTROPHORETIC MOBILITY OF MYCOBACTERIUM AVIUM COMPLEX ORGANISMS. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 70(9):5667-5671, (2004).
Impact/Purpose:
1)Develop an improved method(s) for isolating and/or detecting nontuberculous mycobacteria from potable water. 2)Determine the best DNA fingerprinting method to use to determine genetic relatedness with clinical and environmental isolates.
Description:
The electrophoretic mobilities (EPMs) of thirty Mycobacterium avium Complex (MAC) organisms isolated from clinical and environmental sources were measured in 9.15 mM KH2PO4 buffered water. The EPMs of fifteen clinical isolates ranged from -1.9 to -5.0 µm cm V-1 s-1 with a mean of -3.1 µm cm V-1 s-1, and the EPMs of fifteen environmental isolates ranged from -1.9 to -4.6 µm cm V-1 s-1 with a mean of -3.3 µm cm V-1 s-1 at pH 7. The EPM of MAC organisms were significantly more negative than other waterborne pathogens (E. coli 0157H:57, Vibrio cholerae, Cryptosporidium parvum) measured under similar experimental conditions.