You are here:
LIQUID CHROMATOGRAPHY DETERMINATION OF ANTI-ANDROGEN VINCLOZOLIN AND ITS METABOLITES IN RAT SERUM
Citation:
SierraSantoyo, A., H A. Barton, AND M F. Hughes. LIQUID CHROMATOGRAPHY DETERMINATION OF ANTI-ANDROGEN VINCLOZOLIN AND ITS METABOLITES IN RAT SERUM. JOURNAL OF CHROMATOGRAPHY: B BIOMEDICAL SCIENCES AND APPLICATIONS. Elsevier Science BV, Amsterdam, Netherlands, 809:105-110, (2004).
Impact/Purpose:
The objective of this study was to develop a chromatographic method for the analysis of the anti-androgen vinclozolin (V) and its butenoic acid (M1) and enanilide (M2) metabolites in rat serum.
Description:
The objective of this study was to develop a chromatographic method for the analysis of the anti-androgen vinclozolin (V) and its butenoic acid (M1) and enanilide (M2) metabolites in rat serum. V, M1, M2 and M3 were resolved using an HPLC gradient program with a mobile phase consisting of 60-75% methanol:acetonitrile (70:30) and 0.05 M phosphate buffer (PB) pH 3.3 at 1 ml/min, a C18 column, and a wavelength of 212 nm. V and its metabolites were extracted with acetonitrile from spiked incubates of PB pH 7.4 and rat serum, after diluting samples (1:4) with PB pH 3.3, to limit methodological hydrolysis of analytes. Recoveries of analytes ranged from 85-105%. V was hydrolyzed to M1 and M2 after incubation in PB pH 7.4 and rat serum. M1 was the predominant metabolite detected in both conditions. This method may be used for the analysis of V and its metabolites in pharmacokinetic studies, which could lead to the development of a physiologically-based pharamcokinetic model for the anti-androgen V.