Citation:

Schwartz, E., S. V. Trinh, AND K. M. Scow. MEASURING GROWTH OF A PHENANTHRENE DEGRADING BACTERIAL INOCULUM IN SOIL WITH A QUANTITATIVE COMPETITIVE POLYMERASE CHAIN REACTION METHOD. (R825433). FEMS Microbiology Ecology 34(1):1-7, (2000).

Description:

We measured growth of a phenanthrene-degrading bacterium, Arthrobacter, strain RP17, in Forbes soil, amended with 500 g g⁻¹ phenanthrene using a quantitative competitive polymerase chain reaction method. The inoculum, which was not indigenous to Forbes soil, grew from 5.55×10⁵ colony forming units (cfu) g⁻¹ to 1.97×10⁷ cfu g⁻¹ within 100 h after the cells were added to the soil. Maximum population density was reached before the highest degradation rate was observed 150 h after the cells were added to soil. Population density remained stable even after 56% of the phenanthrene had mineralized. This study is one of the few documented examples of growth by a non-indigenous bacterium in a non-sterile soil amended with a pollutant.

Author Keywords: Quantitative polymerase chain reaction; Phenanthrene degradation; Bioaugmentation; Bioremediation

Record Details:

Record Type:DOCUMENT( JOURNAL/ PEER REVIEWED JOURNAL)

Product Published Date:01/01/2000

Record Last Revised:12/22/2005

Record ID: 84823

Show Additional Record Data

Document IDs:

EPA Number :R825433