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LANTHANIDE ENHANCE LUMINESCENCE (LEL) WITH ONE AND TWO PHOTON EXCITATION OF QUANTUM DYES LANTHANIDE (III) - MACROCYCLES
Citation:
Leif, R. C., M. C. Becker, A. Bromm, N. Chen, A. Cowan, L. M. Vallarino, AND S. Yang. LANTHANIDE ENHANCE LUMINESCENCE (LEL) WITH ONE AND TWO PHOTON EXCITATION OF QUANTUM DYES LANTHANIDE (III) - MACROCYCLES. Presented at SPIE Meeting 2004: The International Society for Optical Engineering, San Jose, CA, January 24-29, 2004.
Description:
Title: Lanthanide Enhance Luminescence (LEL) with one and two photon excitation of Quantum Dyes? Lanthanide(III)-Macrocycles
Principal Author:
Robert C. Leif, Newport Instruments
Secondary Authors:
Margie C. Becker, Phoenix Flow Systems
Al Bromm, Virginia Commonwealth University
Nanguang Chen , University of Connecticut
Ann Cowan, University of Connecticut
Lidia. M. Vallarino, Virginia Commonwealth University
Sean Yang, Newport Instruments
Robert M. Zucker, Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711
This abstract of a proposed presentation does not necessarily reflect EPA policy
Abstract:
Improvements in the Lanthanide Enhance Luminescence (LEL) protocol have facilitated the use of the recently synthesized Eu(III)-macrocycle-mono-isothiocyanate, Quantum Dye?, as a label for direct staining of cells and indirect staining as a Streptavidin conjugate to observe apoptotic and S phase cells. Preliminary studies indicate that europium Quantum Dye stained cells can be observed by both conventional UV laser excited and by infrared two photon confocal microscopy. An enhancer has been found that enables the observation of simultaneous emissions from both the europium and terbium ions.