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THE HISTOLOGY OF FITC AND FITC-17B ESTRADIOL IN CUNNER, TAUTOGOLABRUS ADSPERSUS, AFTER SUBCUTANEOUS IMPLANTATION IN A CONTROLLED RELEASE MATRIX
Citation:
Horowitz, D B., G Zaroogian, R E. GutjahrGobell, AND L J. Mills. THE HISTOLOGY OF FITC AND FITC-17B ESTRADIOL IN CUNNER, TAUTOGOLABRUS ADSPERSUS, AFTER SUBCUTANEOUS IMPLANTATION IN A CONTROLLED RELEASE MATRIX. Presented at Society of Environmental Toxicology & Chemistry Annual Meeting, Nashville, TN, November 12-16, 2000.
Description:
To mimic environmental exposures in studying the effects of estrogenic chemicals on fish reproduction, a slow-release mechanism is needed to deliver a continuous dosing of chemicals over the course of a long-term study. The effects of slow-release chemical exposures via a subcutaneously implanted matrix were evaluated in cunner, Tautogolabrus adspersus. Two fluorescent dyes, fluorescein isothiocyanate (FIT C) and FITC conjugated with 17 -estradiol (FITC-E2), were bound to ethylcellulose in ethoxylated caster oil and implanted dorsally via subcutaneous injection. Fish were sampled at 1,3,7, 14,23, and 43 days post implantation. Strong fluorescence was observed grossly at the site of implantation throughout the study. Liver, gonad, and heart tissues were processed for histology through a rapid paraffin embedding regime and kept in the dark to preserve fluorescence for microscopic evaluation and analysis. Tissue fluorescence was observed using a Zeiss microscope equipped with epifluorescence. Special histochemical stains (Masson's Trichrome Stain, Verhoeff's Elastic Stain with Van Gieson's Counter Stain, and Gomori's Stain for Reticular Fibers) were used to characterize the tissues affected by the FITC and FITC-E2. Observations of fluorescence in the tissue sections showed accumulations through day 43 post implantation surrounding the gonads, around blood vessels in the liver, and within the heart. These findings demonstrate a slow-release of chemicals from the dorsal implantation site to these organs.