Citation:

Morrison, C. N., J Starr, AND E M. Ulrich. ANALYSIS OF SELECTED PYRETHROID PESTICIDES USING REVERSE PHASE HIGH PRESSURE LIQUID CHROMATOGRAPHY/UV. Presented at Society of Environmental Toxicology & Chemistry, Kennesaw, GA, March 25-27, 2004.

Impact/Purpose:

The goal of this task is to contribute to a better understanding of human exposure to pesticides, especially for small children by developing methods to characterize sources and pathways in and around the residential environment. We will support the science behind FQPA and assist the Office of Pesticide Programs (OPP) in the development of guidelines for the assessment of residential exposure to pesticides. Specific research objectives include: (i) to evaluate and develop methods for measuring pesticides in air using passive/diffusive samplers. Assess and refine devices for the collection of surface transferable pesticide residues and to establish transfer efficiencies; (ii) to develop and apply analytical methods for new and emerging pesticides using both gas and liquid chromatographic methods in support of the National Exposure Research Laboratory's (NERL) Human Exposure Measurement Project; and, (iii) to conduct pilot studies investigating chiral chromatographic methods.

Description:

This research was conducted in cooperation with EPA Region 4 in Athens, GA to develop a method to analyze selected pyrethroid pesticides using Reverse Phase-High Pressure Liquid Chromatography (HPLC). This HPLC method will aid researchers in separating and identifying these pyrethroids. The method was developed for Type I pyrethroids without a cyano group (allethrin, permethrin, resmethrin) and Type II pyrethroids with a cyano group (cypermethrin and fenvalerate). The pyrethroids were separated on a Hewlett Packard model 1090 using a Phenomenex C-18 100 x 2.0 mm SYNERGI 4u Hydro-RP 80A column. The flow rate was set at 0.5 mL/min. at 35 degrees C using two mobile phases consisting of acetonitrile-distilled water (1:1, v/v) and methanol-acetonitrile-distilled water (0.5:0.5:1, v/v) with 10 uL injections. The ratios of aqueous to organic mobile phase evaluated ranged from 10:90 to 60:40. Mobile phase conditions of 20% water and 80% acetonitrile separated allethrin (2.207 minutes), resmethrin isomers (7.997, 8.414 minutes), and permethrin isomers (9.631, 11.326 minutes), respectively. Analysis of the pyrethroids separately allows us to hypothesize that, in an unknown sample, complete separation occurs.

Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy. Mention of trade names of commercial products does not constitute endorsement or recommendation for use.

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