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THE EFFECTS OF A HIGH ANIMAL FAT DIET ON THE INDUCTION OF ABERRANT CRYPT FOCI IN THE COLONS OF MALE F344/N RATS EXPOSED TO TRIHALOMETHANES IN THE DRINKING WATER
Citation:
Geter, D. R., M H. George, S. R. Kilburn, G. D. HugginsClark, AND A B. DeAngelo. THE EFFECTS OF A HIGH ANIMAL FAT DIET ON THE INDUCTION OF ABERRANT CRYPT FOCI IN THE COLONS OF MALE F344/N RATS EXPOSED TO TRIHALOMETHANES IN THE DRINKING WATER. TOXICOLOGY LETTERS 147(3):245-252, (2004).
Description:
The Effects of a High Animal Fat Diet on the Induction of Aberrant Crypt Foci in the Colons of Male F344/N Rats Exposed to Trihalomethanes in the Drinking Water
Abstract
Aberrant crypt foci (ACF), identified as the putative precursor lesion in the development of colon cancer, were induced by brominated trihalomethanes (THMs) administered in the drinking water of rats. To investigate whether ACF induced by THMs could be promoted by a diet high in saturated animal fat (HF), male F44/N rats were exposed to 0.5, 0.7, 0.9 or 1.1 g/L of trichloromethane (TCM), bromodichloromethane (BDCM), dibromochloromethane (DBCM) and tribromomethane (TBM), respectively, in drinking water. Animals given a single 15 mg/kg dose of azoxymethane (AOM) served as the positive control. Half of the animals in each treatment group were fed Purina 5001 diet supplemented with 19% animal fat (Western or high fat diet) or a normal (4.5% fat) diet. After 26 weeks of treatment, the colons were excised and examined for ACF. A statistically significant increase in the number of ACF was noted with increasing bromination. No difference in ACF was noted between animals fed a normal or high fat diet and exposed to TCM, BDCM or DBCM. However, animals exposed to TBM and fed a high fat diet showed a significant and near 2-fold increase in ACF when compared to TBM exposed animals fed a normal diet.