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DISTRIBUTION OF PCB 84 ENANTIOMERS IN C57BL/6 MICE
Citation:
Lehmler, H. J., D. J. Price, A W. Garrison, W. J. Birge, AND L. W. Robertson. DISTRIBUTION OF PCB 84 ENANTIOMERS IN C57BL/6 MICE. FRESENIUS ENVIRONMENTAL BULLETIN 12(2):254-260, (2003).
Impact/Purpose:
Extend existing model technologies to accommodate the full range of transport, fate and food chain contamination pathways, and their biogeographical variants, present in agricultural landscapes and watersheds. Assemble the range of datasets needed to execute risk assessments with appropriate geographic specificity in support of pesticide safety evaluations. Develop software integration technologies, user interfaces, and reporting capabilities for direct application to the EPA risk assessment paradigm in a statistical and probabilistic decision framework.
Description:
Nineteen of the 209 possible PCB congeners exist as pairs of stable rotational isomers that are enantiomeric to each other. A racemic mixture of PCB atropisomers is present in technical PCB mixtures, thus raising concerns about enantioselective distribution, metabolism, and disposition of these congeners. We investigated the distribution and enantiomeric fractions (EF) of PCB 84 in untreated female C57B1/6 mice. PCB 84 was injected intraperitoneally with 600 mumol/kg body weight, and the EFs were determined by chiral gas chromatography in the liver, brain, lung, heart, spleen and kidney after three or six days. The EFs in brain, liver, lung and heart were significantly different from the racemic PCB 84 standard at days three and six, with an enrichment of (+)-PCB 84 in all four tissues. A significant enrichment of (+)-PCB 84 in the kidney was observed for day six. No significant difference was observed for the spleen on both time points. Tissue EFs for the brain showed the highest EF, whereas the EFs in the spleen were almost identical with the PCB 84 standard. The EFs did not change significantly between day three and six, suggesting that the differential distribution of (+)-PCB 84 may primarily occur during the initial distribution phase.