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PALATAL DYSMORPHOGENESIS: QUANTITATIVE RT-PCR
Citation:
Held, G A. AND B D. Abbott. PALATAL DYSMORPHOGENESIS: QUANTITATIVE RT-PCR. Chapter 22, Humana Press Incorporated, Totowa, NJ, 136:203-217, (2000).
Description:
ABSTRACT
Palatal Dysmorphogenesis : Quantitative RT-PCR
Gary A. Held and Barbara D. Abbott
Reverse transcription PCR (RT-PCR) is a very sensitive method for detecting mRNA in tissue samples. However, as it is usually performed it is does not yield quantitative results. A modified RT-PCR technique, competitive RT-PCR is capable of giving quantitative results. Competitive RT-PCR makes use of a synthetic RNA molecule which contains the same primers as the naturally occurring mRNA of interest. This synthetic molecule is used as an internal standard (IS) in each RT-PCR reaction and competes with the naturally occurring mRNA, allowing the amount of mRNA present to be estimated. This chapter presents a detailed protocol for performing competitive RT-PCR in the context of studying gene expression in the developing palate.