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COMPUTER-ASSISTED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT WITH APPLICATIONS TO THE ISOLATION AND ANALYSIS OF PHYTOPLANKTON PIGMENTS. (R826944)
Citation:
Van, H. E. AND C. S. Thomas. COMPUTER-ASSISTED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT WITH APPLICATIONS TO THE ISOLATION AND ANALYSIS OF PHYTOPLANKTON PIGMENTS. (R826944). JOURNAL OF CHROMATOGRAPHY A. American Chemical Society, Washington, DC, 910(1):31-49, (2001).
Description:
We used chromatography modeling software to assist in HPLC method development, with the goal
of enhancing separations through the exclusive use of gradient time and column temperature. We
surveyed nine stationary phases for their utility in pigment purification and natural sample analysis. For
purification, a complex algal matrix was separated on an efficient monomeric column, from which
partially purified fractions were collected and purified on polymeric columns that exaggerated
resolution between pigments of interest. Additionally, we feature an HPLC method that is simple,
fast, demonstrates excellent transferability and is ideal for quantitative analysis of pigments in dilute
natural water samples.