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SEPARATION AND CHARACTERIZATION OF TETROL METABOLITES OF BENZO[A]PYRENE-DNA ADDUCTS USING HPLC AND SOLID-MATRIX ROOM TEMPERATURE LUMINESCENCE. (R824100)
Citation:
Tjioe, S. W. AND R. J. Hurtubise. SEPARATION AND CHARACTERIZATION OF TETROL METABOLITES OF BENZO[A]PYRENE-DNA ADDUCTS USING HPLC AND SOLID-MATRIX ROOM TEMPERATURE LUMINESCENCE. (R824100). TALANTA. Elsevier Science Ltd, New York, NY, 42(1):59-64, (1995).
Description:
Abstract
Four tetrols of benzo[a]pyrene-DNA adducts were separated using reversed-phase high performance liquid chromatography. Chromatographic fractions containing a given tetrol were readily characterized with solid-matrix room temperature luminescence techniques. Solid-matrix fluorescence and phosphorescence spectra at picogram amounts of a tetrol were easily obtained. Tetrol fractions collected based on retention times, but with no response from the ultraviolet detector, were characterized by solid-matrix luminescence spectra at very small quantities.