Citation:

Girard, J. P., C. Ferrua, AND D. Pesando. EFFECTS OF TRIBUTYLTIN ON CA2+ HOMEOSTASIS AND MECHANISMS CONTROLLING CELL CYCLING IN SEA URCHIN EGGS (R823881). AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, 38(4):225-239, (1997).

Description:

Abstract

Tributyltin (TBT) is one of the widespread organotins in the marine environment: we have investigated its cellular targets in the eggs of the marine invertebrate sea urchin Paracentrotus lividus. TBT was used at concentrations ranging from 10^-9 to 10^-4 M. The lipophilic character of TBT makes that the magnitude of the effects varied according to the concentration of egg used. Concentrations of TBT being without effect on egg fertilisation inhibited first and second cleavage in a dose-dependent manner. Concentrations of TBT which block egg cleavage did not alter egg membrane permeabilities to Ca²⁺ and Na⁺. TBT inhibited intracellular ATP-driven sequestration of Ca²⁺ into reticular compartment but did not provoke Ca²⁺ leakage from these compartment. Blockade of eggs cleavage by TBT is accompanied by an inhibition of protein synthesis (³⁵S-methionine incorporation) and DNA synthesis (³H-thymidine incorporation). Protein phosphorylation (³²P incorporation) and histone H1 kinase activity were inhibited when cell cycling was arrested by organotin. Results are discussed in terms of concentration of TBT per egg protein in order to compare the particular sensitivity of the numerous cellular process affected by TBT.

Author Keywords: Organotin; TBT; Sea urchin eggs; Calcium homeostasis; Cell cycle

Record Details:

Record Type:DOCUMENT( JOURNAL/ PEER REVIEWED JOURNAL)

Product Published Date:06/01/1997

Record Last Revised:12/22/2005

Record ID: 66827

Show Additional Record Data

Document IDs:

EPA Number :R823881