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ENHANCED ENZYMATIC REMOVAL OF CHLOROPHENOLS IN THE PRESENCE OF CO-SUBSTRATES. (R823847)
Citation:
Roper, J. C., J. M. Sarkar, J. Dec, AND J. Bollag. ENHANCED ENZYMATIC REMOVAL OF CHLOROPHENOLS IN THE PRESENCE OF CO-SUBSTRATES. (R823847). WATER RESEARCH. Elsevier Science Ltd, New York, NY, 29(12):2720-2724, (1995).
Description:
The effect of reactive co-substrates such as guaiacol and 2,6-dimethoxyphenol on the removal of chlorinated phenols by horseradish peroxidase (HRP) and a
laccase from the fungus Trametes versicolor was investigated. Addition of 50 mM guaiacol enhanced the precipitation of 4-chlorophenol, 2,4-dichlorophenol, and
2,4,5-trichlorophenol with peroxidase by 12, 32 and 65%, respectively, and with laccase by 20, 32 and 80%, respectively. Addition of 10 mM
2,6-dimethoxyphenol enhanced the precipitation of 2,4,5-trichlorophenol by 90% with peroxidase and by 98% with laccase. Products from the reaction of
2,6-dimethoxyphenol and peroxidase were filtered to exclude compounds of a molecular weight greater than 500. Incubation of the resulting enzyme-free filtrate with
a solution of unreacted 2,4,5-trichlorophenol caused precipitation and a 72% removal of the 2,4,5-trichlorophenol. Chlorophenol precipitation in the presence of
co-substrates may be a useful strategy for improving the efficiency of enzymatic decontamination methods, particularly in the case of heterogeneous pollution.
Author Keywords: chlorophenols; peroxidase; laccase; co-substrate; copolymerization; enzymatic decontamination; coupling reactions; 2,4-dichlorophenol;
2,4,5-trichlorophenol; decontamination method