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USING STRUCTURAL EFFECTS ON THE ORGANIZATION OF THE CYTOSKELETON OF RAINBOW TROUT HEPATOCYTES TO SORT PATHWAYS OF REACTIVE TOXICITY
Citation:
Flynn, K. M., P. K. Schmieder, AND R. D. Johnson. USING STRUCTURAL EFFECTS ON THE ORGANIZATION OF THE CYTOSKELETON OF RAINBOW TROUT HEPATOCYTES TO SORT PATHWAYS OF REACTIVE TOXICITY. Presented at Society of Toxicology, Baltimore, MD, March 21-25, 2004.
Description:
Quinones have been shown to be more acutely toxic to aquatic organisms than chemicals that are not capable of either direct interaction with cellular nucleophiles or potentially metabolized free radicals. For the development of accurate QSAR models, in vitro toxicity assays are needed that can readily distinguish more toxic arylating chemicals from those that primarily redox cycle. The cytoskeleton has multiple components providing an array of potential targets for direct arylation or oxidative stress induced by redox cycling. Four quinones served as model chemicals used to determine if alteration in the organization of the cytoskeleton of rainbow trout hepatocytes could adequately distinguish toxic pathways. - - - Redox cycling quinones alter intermediate filaments more than other components, and only after much longer exposures. Electrophilic chemicals differentially affect the various components of the cytoskeleton, and a cytoskeletal endpoint assay shows promise in its ability to distinguish reactive pathways.