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COMPARISON OF TISSUE CULTURE AND ANIMAL MODELS FOR ASSESSMENT OF CRYPTOSPRIDIUM PARVUM INFECTION
Citation:
Slifko, T. R., D. Huffman, B. Dussert, J H. Owens*, W Jakubowski, J. B. Rose, AND C. N. Haas. COMPARISON OF TISSUE CULTURE AND ANIMAL MODELS FOR ASSESSMENT OF CRYPTOSPRIDIUM PARVUM INFECTION. Hajduk, S.L. (ed.), EXPERIMENTAL PARASITOLOGY. Academic Press, New York, NY, 101(2-3):97-106, (2002).
Description:
Data from three different disinfection studies using both cell culture and mouse infectivity to assess Cryptosporidium parvum inactivation were evaluated in a total of 35 comparison including process controls and treated samples. C. parvum infectivity in the in vitro FDM-MPN assay was used to determine the dose needed to infect 50% of the cell cultures (ID50) and was compared to ID50 in the in vivo mouse model. Average ID50's developed using the logit dose response method for cell culture and mice were 8 and 107, respectively. Correlation (r) between cell culture and mouse infectivity was 0.9185 (95% CI=0.8434 to 0.9584, p>0.0001). While assays showed similar infectivity, cell culture appeared more sensitive, attributing to the lower ID50s. T test comparison of the inactivation predicted by cell culture and mice determined no significant difference between the two assays (P=0.4481; t+0.7718, 95% CI=-0.1625 to 0.3558; n=24). These results demonstrate that the cell culture FDM-MPN assay is a reliable alternative to using mice for the study of the zoonotic Cryptosporidium parvum genotype.