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A METHOD TO DETECT VIABLE HELICOBACTER PYLORI BACTERIA IN GROUNDWATER
Citation:
Flanigan, D J. AND M R. Rodgers. A METHOD TO DETECT VIABLE HELICOBACTER PYLORI BACTERIA IN GROUNDWATER. ACTA HYDROCHIM 31(1):45-48, (2003).
Impact/Purpose:
Investigate the occurrence of Aeromonas bacteria in potable water derived from both surface and ground water sources. Develop improved method(s) for the detection of culturable H. pylori in environmental samples and investigate the occurrence of H. pylori in potable waters.
Description:
The inability to detect the presence of viable Helicobacter pylori bacteria in environmental waters has hindered the public health community in assessing the role water may playin the transmission of this pathogen. This work describes a cultural enrichment method coupled with an H. pylori-specific PCR to identify these bacteria in water. While far from perfected at the present time, this represents an exciting new approach to studying the significance of water as a transmission mechanism for H. pylori. Evidence is presented that indicates culturable H. pylori bacteria were found using this enrichment/PCR method in a local groundwater source.