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REMOVAL OF AMMONIA TOXCITY IN MARINE SEDIMENT TIES: A COMPARISON OF ULVA LACTUCA, ZEOLITE AND AREATION METHODS
Citation:
Burgess, R M., M Pelletier, K T. Ho, J R. Serbst, S Ryba, A Kuhn, M. M. Perron, P. Raczelowski, AND M Cantwell. REMOVAL OF AMMONIA TOXCITY IN MARINE SEDIMENT TIES: A COMPARISON OF ULVA LACTUCA, ZEOLITE AND AREATION METHODS. MARINE POLLUTION BULLETIN 46:607-618, (2003).
Description:
Ammonia is suspected of causing some of the toxicity observed in marine sediment toxicity tests because it is sometimes found at elevated concentrations in marine interstitial waters. In marine waters, ammonia exists as un-ionized ammonia (NH3) and ammonium (NH4+) which combine to equal total ammonia (NHx). Toxicity Identification Evaluations (TIEs) can be used to determine the specific toxicant(s), including ammonia, causing toxicity observed in marine sediments. Two primary TIE manipulations are available for characterizing and identifying ammonia in marine sediments: Ulva lactuca addition and zeolite addition. In this study, we compared the efficacy of these methods to (1) remove NHx and NH3 from overlying and interstitial waters and (2) reduce toxicity to the amphipod Ampelisca abdita and mysid Americamysis bahia using both spiked and environmentally contaminated sediments. The utility of aeration for removing NHx and NH3 during a marine sediment TIE was also evaluated preliminarily. The U. lactuca and zeolite addition methods performed similarly well at removing spiked NHx and NH3 from overlying and interstitial waters compared to an unmanipulated sediment. Toxicity to the amphipod was reduced approximately the same by both methods. However, toxicity to the mysid was most effectively reduced by the U. lactuca addition indicating this method functions best with epibenthic species exposed to ammonia in the water column. LC50s based on measured concentrations of NHx and NH3 were consistent by species regardless of overlying water or interstitial water exposure and were, with one exception, within the range of LC50s reported in the literature. Both methods also operated equally well reducing overlying water concentrations of NHx and NH3 from environmentally contaminated sediment with a mean differences between the manipulations of only 8%. Aeration removed NHx and NH3 from seawater when the pH was adjusted to 10; however, very little ammonia was removed at ambient pHs (~8.0). Due to this and the technical difficulties associated with maintaining elevated pHs in a toxicity test this manipulation was deemed unsuitable for sediment TIEs. This comparison demonstrates both U. lactuca and zeolite addition methods are effective TIE tools for reducing the concentrations and toxicity of ammonia in whole sediment toxicity tests.