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DETERMINING EOSIN AS A GROUNDWATER MIGRATION TRACER BY CAPILLARY ELECTROPHORESIS/LASER-INDUCED FLUORESCENCE USING A MULTIWAVELENGTH LASER
Citation:
Brumley, W C. AND J. W. Farley. DETERMINING EOSIN AS A GROUNDWATER MIGRATION TRACER BY CAPILLARY ELECTROPHORESIS/LASER-INDUCED FLUORESCENCE USING A MULTIWAVELENGTH LASER. ELECTROPHORESIS 24(14):2335-2339, (2003).
Impact/Purpose:
Provide state-of-the-science sampling, analysis, separation, and detection methods to allow rapid, accurate field and laboratory analyses of contaminated soils, sediments, biota, and groundwater to support Superfund clean-up decisions. Apply state-of-the-science methods in chemical analysis and data interpretation (e.g., mass spectral interpretation) to actual problems of OSWER, the Regions, and the States, in cooperation with the Las Vegas Technical Support Center as well as by direct contacts with Regional and State employees. Provide technical advice and guidance to OSWER using the environmental chemistry expertise (e.g., mass spectrometry, analytical methods development, clean-up methodology, inorganics, organometallics, volatile organics, non-volatile organics, semi-volatile organics, separation technologies, etc.) found within the branch.
Technical research support for various projects initiated either by Regions/Program Offices or ECB scientists. While these efforts will support the Regions and Program Offices, they cannot be predicted or planned in advance, and may serve multiple duty (e.g., solve real-world problems, serve to ground-truth analytical approaches that ECB is developing, transfer new technology). Many of the activities in this task support requests involving enforcement decisions and therefore are categorized as "environmental forensics".
Description:
Groundwater migration remains an important contributor in determining the distribution and fate of environmental pollutants originating from various waste sites or in understanding fate and transport .[ 1- 3] .Groundwater tracers are often used to determine the flow of groundwater. The tracers can be fluorescent dyes, in which case the use of synchronous scanning spectrofluorimetry is the technique of choice to determine the tracers in water or other samples. The U.S. EPA National Exposure Research Laboratory (Las Vegas) is interested in developing and applying 8 analytical tools that can strengthen the regulatory application of analysis. Among those tools under investigation is capillary electrophoresis/laser-induced fluorescence detection (CE/LIF), which is particularly well-suited to the analysisofwater-soluble and fluorogenic compounds. Fluorescent dyes are a convenient choice for tracers because of the ease of sensitive detection. Spectrofluorimetry , high performance liquid chromatography (HPLC)/UV or fluorescence detection, and capillary LC/fluorescence detection have also been used [2-6]. For HPLC or capillary LC, the retention of dye analytes is enhanced using ion-pairing techniques. Logically, applications of capillary electrophoresis/laser-induced fluorescence (CE/LIF) detection should be ideal for the determination of anionic ( or cationic) dyes. In free-zone CE, the dyes are separated very simply on the basis of their mobilities in aqueous buffers. Among several reports on CE/LIF detection techniques are two early papers based on high-sensitivity detection of fluorescent dyes [7,8] and more recent work applied directly to groundwater migration studies [9-12] .General reviews of environmental applications of CE and sample handling were published [13-14], and more recently updated reviews appear regularly [15] Additional environmental