You are here:
DEVELOPMENT OF CODOMINANT MARKERS FOR IDENTIFYING SPECIES HYBRIDS
Citation:
Tranah, G. J., M J. Bagley, J. J. Agresti, AND B. May. DEVELOPMENT OF CODOMINANT MARKERS FOR IDENTIFYING SPECIES HYBRIDS. CONSERVATION GENETICS 4(4):537-541, (2003).
Impact/Purpose:
The objective of this task is to develop molecular indicators to evaluate the integrity and sustainability of aquatic fish, invertebrate, and plant communities (GPRA goal 4.5.2). Specifically, this subtask aims to evaluate methods for the measurement of:
fish and invertebrate community composition, especially for morphologically indistinct (cryptic) species
population genetic structure of aquatic indicator species and its relationship to landscape determinants of population structure (to aid in defining natural assessment units and to allow correlation of population substructure with regional stressor coverages)
genetic diversity within populations of aquatic indicator species, as an indicator of vulnerability to further exposure and as an indicator of cumulative exposure
patterns of temporal change in genetic diversity of aquatic indicator species, as a monitoring tool for establishing long-term population trends.
Description:
Herein we describe a simple method for developing species-diagnostic markers that would permit the rapid identification of hybrid individuals. Our method relies on amplified length polymorphism (AFLP) and single strand conformation polymorphism (SSCP) technologies, both of which can be performed in any molecular biology facility using standard laboratory equipment. We demonstrate the utility of the AFLP-SSCP method by developing three taxon-specific markers that will be suitable for monitoring introgression in endangered Klamath basin suckers.