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L166P MUTANT DJ-1, CAUSATIVE FOR RECESSIVE PARKINSON'S DISEASE IS DEGRADED THROUGH THE UBIQUITIN-PROTEASOME SYSTEM
Citation:
Miller, D. W., R. Ahmad, S. Hague, M. J. Baptista, R. CanetAviles, C. McLendon, D. M. Carter, P. P. Zhu, J. Stadler, J. Chandran, G R. Klinefelter, C. Blackstone, AND M. R. Cookson. L166P MUTANT DJ-1, CAUSATIVE FOR RECESSIVE PARKINSON'S DISEASE IS DEGRADED THROUGH THE UBIQUITIN-PROTEASOME SYSTEM. JOURNAL OF BIOLOGICAL CHEMISTRY 278(38):36588-36595, (2003).
Description:
Abstract
Mutations in a gene on chromosome 1, DJ-1, have been reported recently to be associated with recessive, early-onset Parkinson's disease. Whilst one mutation is a large deletion that is predicted to produce an effective knockout of the gene, the second is a point mutation, L166P, whose precise effects on protein function are unclear. In the present study , we show that L166P destabilizes DJ-1 protein and promotes its degradation through the ubiquitin-proteasome system. A double mutant (K130R, L166P) was more stable than L166P, suggesting that this lysine residue contributes to stability of the protein. Subcellular localization was broadly similar for both wild type and L166P forms of the protein, indicating that the effect of the mutation is predominantly on protein stability. These observations are reminiscent of other recessive gene mutations that produce an effective loss of function. The L166P mutation has athe simple effect of promoting DJ-1 degradation, thereby reducing net DJ-1 protein within the cell.