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USING BASE-SPECIFIC SALMONELLA TESTER STRAINS TO CHARACTERIZE THE TYPES OF MUTATION INDUCED BY BENZIDINE AND BENZIDINE CONGENERS AFTER REDUCTIVE METABOLISM
Citation:
Claxton, L D., T. J. Hughes, AND K. T. Chung. USING BASE-SPECIFIC SALMONELLA TESTER STRAINS TO CHARACTERIZE THE TYPES OF MUTATION INDUCED BY BENZIDINE AND BENZIDINE CONGENERS AFTER REDUCTIVE METABOLISM. FOOD AND CHEMICAL TOXICOLOGY 39(12):1253-1261, (2000).
Description:
Abstract
Benzidine, 4-aminobiphenyl, 3,3'-dichlorobenzidine HCl, 3,3'-dimethylbenzidine, 3,3'- dimethoxybenzidine and benzidine congener-based dye trypan blue were mutagenic in Salmonella typhimurium TAl 00 only with metabolic activation. It was found that a hamster liver 89 preparation with glucose-6-phosphate dehydrogenase, FMN, NADH and 4 times glucose 6- phosphate (Prival activation system) gave a stronger mutagenic response than the conventional plate incorporation with rat 89 activation mixture for all the compounds tested. Compounds were tested further in Xenometrix strains of S. typhimurium:TA7001, TA7002, TA7003, TA7004, TA7005 and TA7006 with the Prival metabolic activation system. Only benzidine was weakly mutagenic in TA7005 .4-Aminobiphenyl was mutagenic in TA7002, TA7004, TA7005, and TA7006. 3,3'-Dichlorobenzidine was weakly mutagenic in TA7004. 3,3'-Dimethylbenzidine, 3,3'-dimethoxybenzidine and trypan blue were not mutagenic in any of the Xenometrix strains. It was concluded that benzidine induced some CG-> AT transversions in addition to frame shift mutations. 4- Amino biphenyl induced T A-> A T , CG-> A T, and CG->GC transversions as well as GC->AT transitions. 3,3'-DicWorobenzidine induced only GC->AT transitions. The mutagenic specificities of 3,3'-dimethylbenzidine, 3,3'-dimethoxybenzidine and trypan blue were not determined in this base-substitution mutagen detection system because their activity was mainly due to frameshift mechanisms.
Keywords: Salmonella typhimurium, benzidine congeners, mutagenic spectra, Prival modification, mutagenicity tests, carcinogens, dyes, histidine operon mutations, benzidine (92-87-5); 4-aminobiphenyl (92-67-1); 3,3'-dichlorobenzidine (91-94-1); 3,3'-dimethylbenzidine (119-93- 7); dimethoxybenzidine (119-90-4); trypan blue (72-57-1)
This abstract does not necessarily reflect the US EPA policy.