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RATE AND CAPACITY OF HEPATIC MICROSOMAL RING HYDROXYLATION OF PHENOL TO HYDROQUINONE AND CATECHOL IN RAINBOW TROUT
Citation:
Kolanczyk, R C. AND P. K. Schmieder. RATE AND CAPACITY OF HEPATIC MICROSOMAL RING HYDROXYLATION OF PHENOL TO HYDROQUINONE AND CATECHOL IN RAINBOW TROUT. AQUATIC TOXICOLOGY 176/1-2:77-90, (2002).
Description:
Rainbow trout (Oncorhynchus mykiss) liver microsomes were used to study the rate of ring-hydroxylation of phenol PH) by directly measuring the production of hydroquinone (HQ), the primary metabolite, and catechol (CAT), a secondary metabolite. An HPLC method with integrated ultraviolet (UV) and electrochemical detection (ECD) was used for metabolite identification and quantification at low concentrations...These experiments provide a means to directly quantify metabolic bioactivation potential in fish species. This work initiates a series of studies to compare activation rate and capacity across aquatic species, providing a basis for the prediction of metabolic pathways and rates.