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RAPID ANALYSIS OF CYNANURIC ACID IN SWIMMING POOL WATERS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY USING POROUS GRAPHITIC CARBON COLUMN
Citation:
Cantu, R, O M. Evans, AND M L. Magnuson. RAPID ANALYSIS OF CYNANURIC ACID IN SWIMMING POOL WATERS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY USING POROUS GRAPHITIC CARBON COLUMN. 2001, CHROMATOGRAPHIA 53(7/8):454-456, (2001).
Impact/Purpose:
Develop a method to determine the volume of recreational water ingested by relating the quantity imbibed to the level of a chemical biomarker measured in urine.
Description:
An innovative approach is presented for reducing analysis times of cyanuric acid in swimming pool waters by high performance liquid chromatography (HPLC). The HPLC method exploits the unique selectivity of porous graphitic carbon (PGC) to fully resolve cyanuric acid from other pool interferences within 10 minutes. By carefully timing the injections, multiple injections can be made before the end of the initial chromatographic run, more than doubling sample throughout. The method utilizes 95% of a 50 mM phosphate buffer solution (pH 9.1) and 5% methanol (%, v/v) with UV detection at 213 nm. This approach yielded run times rivaling those of the fastest methods using silica columns, and with the benefits of increased sensitivity.