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IDENTIFICATION OF STEREOCHEMICAL CONFIGURATIONS OF CYCLOPENTA[CD]PYRENE-DNA ADDUCTS IN STRAIN A/J MOUSE LUNG AND C3H10T1/2CL8 CELLS
Citation:
Nelson, G B., C. M. Prusiewicz, J A. Ross, R. Sangaiah, L. Ball, AND A. Gold. IDENTIFICATION OF STEREOCHEMICAL CONFIGURATIONS OF CYCLOPENTA[CD]PYRENE-DNA ADDUCTS IN STRAIN A/J MOUSE LUNG AND C3H10T1/2CL8 CELLS. POLYCYCLIC AROMATIC COMPOUNDS 22(3-4):923-931, (2001).
Description:
Identification of Sterochemical Configurations of Cyclopent A[cd]Pyrene DNA Adducts in Strain A/J Mouse Lung and C3H10T1/2CL8 Cells.
Four major and several minor DNA adducts were resolved by 32P-postlabeling analysis of DNA from strain A/J mouse lung and C3H10T1/2CL8 (C3H10T1/2) mouse embryo fibroblasts treated with cyclopenta[cd]pyrene (CPP). The identical pattern of adducts was seen in vivo and in vitro. Co-chromatography of synthetic resolved diastereomers of cis and trans-N2-CPP-
deoxyguanosine-3'-phosphates with the in vivo adducts obtained from strain A/J mouse lung and in vitro adducts obtained from C3H10T1/2 cells allowed identification of the predominant DNA adduct as cis-(3R,4S)-N2-CPP-deoxyguanosine. The second most abundant adduct formed in vivo and in vitro was identified as trans-(3S,4S)-N2-CPP-deoxyguanosine.