You are here:
MULTIPLE ENZYME RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS FOR HIGH RESOLUTION DISTINCTION OF PSEUDOMONAS (SENSU STRICTO) 16S RRNA GENES
Citation:
Porteous, L A., F. Widmer, AND R J. Seidler. MULTIPLE ENZYME RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS FOR HIGH RESOLUTION DISTINCTION OF PSEUDOMONAS (SENSU STRICTO) 16S RRNA GENES. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science BV, Amsterdam, Netherlands, 51:337-348, (2002).
Description:
Pseudomonas specific 16S rDNA PCR amplification and multiple enzyme restriction fragment length polymorphism (MERFLP) analysis using a single digestion mixture of Alu I, Hinf I, Rsa I, and Tru 9I distinguished 150 published sequences and reference strains of authentic Pseudomonas genotypes representing 28 species. Five distinct similarity clusters, containing 65% of the control sequences and strains, were identified. The clusters represented the species P.putida, P. syringae, P. aeruginosa, P. stutzeri, and P. fluorescens but did not solely include these genotypes. MERFLPs from 100% (33/33) control cultures of five Pseudomonas (sensu strictu) species, P.putida, P. syringae, P. aeruginosa, P. stutzeri, and P. fluorescens, were correctly placed into the appropriate phylogenetic clusters. The PCR-MERFLP method was used to genotype 28 Pseudomonas environmental clones and isolates, 67% were included in the five clusters, and cluster analysis identified their relatedness to reference strains.