You are here:
PENTACHLOROPHENOL POTENTIATES BENZO[A]PYRENE DNA ADDUCT FORMATION IN ADULT BUT NOT INFANT B6C3F1 MALE MICE
Citation:
Bordelon, N., K. C. Donnelly, AND S E. George. PENTACHLOROPHENOL POTENTIATES BENZO[A]PYRENE DNA ADDUCT FORMATION IN ADULT BUT NOT INFANT B6C3F1 MALE MICE. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS 37(2):164-172, (2000).
Description:
Abstract
The objective of this study is to determine whether pentachlorophenol (PCP) alters benzo[a]pyrene (B[a]P) induced DNA adduct formation in infant and adult B6C3Fl mice. Mice were exposed to 55 ug B[a]P/g body weight (BW) alone and in combination with several doses of PCP in DMSO. The 32P-postlabeling assay was used to analyze for 7R, 8S, 9S-trihydroxy-1OR-(N2-2'-deoxygaunosyl)-7,8,9, 10-tetrahydro-B[a]P (BPDE-N2G) adducts formed in liver and lung DNA. Hepatic DNA also was analyzed for 8-hydroxy-2'-deoxy- guanosine (8-OHdG) base damage in mice exposed to PCP. 8-OHdG was not detected at any dose of PCP in infant or adult mice. PCP exhibited an antagonistic effect on BPDE-N2G accumulation in infant mice exposed to B[a]P in combination with 50 ug PCP/g BW at both 12 and 24 h. Comparatively, BPDE-N2G adducts were increased in adult mice exposed to binary mixtures at 24 h in both hepatic and lung DNA (p < 0.05). Multiple comparison analysis between infant and adult mice revealed that adduct levels in infants exposed to B[a]P alone or in combination with PCP were not different from those observed in adult mice exposed to B[a]P. However, a significant increase in adducts was observed in adult mice exposed to a combination of B[a]P and PCP compared to all other treatment groups (p < 0.05). These results suggest that PCP alters the metabolism of B[a]P in both infant and adult mice through different mechanisms, and that infants are not susceptible to the potentiative effects of PCP observed in adult mice.
Key words: DNA adducts, age dependent interactions, benzo[a]pyrene,
This abstract does not necessarily reflect US EPA policy.