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CHARACTERIZATION OF THE HEMOLYSIN, STACHYLYSIN, FROM STACHYBOTRYS CHARTARUM
Citation:
Vesper, S J., D. G. Dearborn, M L. Magnuson, I. Yike, AND R A. Haugland. CHARACTERIZATION OF THE HEMOLYSIN, STACHYLYSIN, FROM STACHYBOTRYS CHARTARUM. INFECTION AND IMMUNITY 69(2):912-916, (2001).
Impact/Purpose:
To understand children's risks from exposure to molds in their environment and to explore risk management options for mitigating those risks.
Description:
Stachybotrys chartarum is a toxigenic fungus that has been associated with human health concerns, including pulmonary hemorrhage/hemosiderosis. This fungus produces a hemolysin, stachylysin, which in its monomeric form, has a molecular wieght of 11,920 daltons as determined by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-MS). Heat treatment at 60oC for 30 min inactivated the protein. Stachylysin is composed of about 40% hydrophobic amino acids and contains 2 cysteine residues. Purified stachylysin requires more than 6 h to begin lysing sheep's red blood cells, but by 48 h the treated sheep's red blood cells show pores on the surface.