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CHARACTERIZATION OF THE GLYCOSYLATED ECDYSTEROIDS IN THE HEMOLYMPH OF BACULOVIRUS-INFECTED GYPSY MOTH LARVAE AND CELLS IN CULTURE
Citation:
Kelly, T. J., J. E. Park, C. Masser, AND J. P. Burand. CHARACTERIZATION OF THE GLYCOSYLATED ECDYSTEROIDS IN THE HEMOLYMPH OF BACULOVIRUS-INFECTED GYPSY MOTH LARVAE AND CELLS IN CULTURE. ANNUAL REVIEW OF ENTOMOLOGY 92:51-61, (1995).
Description:
Fourth-instar gypsy moth (Lymantria dispar; Lepidoptera: Lymantriidae) larvae, infected with the gypsy moth baculovirus (LdNPV), show an elevated and prolonged extension of the hemolymph ecdysteroid titer peak associated with molting. The ecdysteroid immunoreactivity associated with this peak elutes as two peaks following HPLC on a C18 reverse-phase column. Both peaks elute in a region more polar than 20-hydroxyecdysone, but less polar than the highly polar ecdysteroid immunoreactivity associated with the apolysis peak of control animals. Glycosylated ecdysteroid standards, produced by in vitro incubation of UDP-glucose with ecdysone or 20-hydroxyecdysone and culture medium from LdNPV-infected gypsy moth cells, show elution times identical to the two immunoreactive peaks. Enzymatic hydrolysis studies verified this identity. The data suggest that the hemolymph of LdNPV-infected L. dispar larvae contains both glucose-conjugated ecdysone and glucose-conjugated 20-hydroxyecdysone which, by analogy with the literature, are presumably 22-O-b-D-glucopyranoside and 20-hydroxyecdysone 22-O-b-D-glucopyranoside