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SUSPENSION CULTURE AND PLANT REGENERATION OF TYPHA LATIFOLIA
Citation:
Estime**, L, M L. O'Shea*, M Borst*, J. Gerrity, AND J. Xing. SUSPENSION CULTURE AND PLANT REGENERATION OF TYPHA LATIFOLIA. Robbins, L. M. (ed.), HORTSCIENCE. American Society for Horticultural Science, 37(2):406-408, (2002).
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Description:
This study is the first reported attempt to generate a growth curve from Typha latifolia L. (broadleaf cattail) callus cells in suspension culture. Several media and hormone combinations were tested for their capacity to induce callus cell formation from T. latifolia leaf sections and both male and female inflorescence spikes. A. T. latifolia callus cell line was successfully established from immature female inflorescence spikes. Callus growth on Gamborgs B5 medium supplemented with 5 mg/L(-1) dicamba and 1 mg/L(-1) BA was superior to other media examined. A growth curve in suspension culture was generated on the most favorable culture medium for callus growth. The mass of the cells increased by 150% by the end of the growth curve. Researchers have reported that this species can remove pollutants from stormwater wetlands very effectively (Breed, 1993: Kadlec and Knight, 1996). T latifolia grows in freshwater marshes, wet swales, streams, ponds, and along lake margins; it is found in all fifty states and is among the most common aquatic plants. It can dominate large areas, especially where water levels fluctuate. In the early spring, it rapidly forms dense colonies that slow down stormwater-associated flows and allow particles to settle into the sediment. It is also known to uptake such nutrients and heavy metals from stormwater wetlands as P, N, Cu, Ni, Zn, and Mg which are then stored in all parts of the plant, including the flower. This makes it an excellent candidate for employment in stormwater constructed wetlands.